Difference between revisions of "Team:SHSBNU China/Parts"

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<img style="width:5vw;height:5vw" src="https://static.igem.org/mediawiki/2018/e/e2/T--SHSBNU_China--Back_To_Top.png"/>
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<p style="font-family: Serif;font-size: 3.5vw; font-weight: bold;text-align: center;margin: 0.5vw 0vw 0.5vw 0vw">ATTRIBUTIONS</p>
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<p style="font-family: Serif;font-size: 3.5vw; font-weight: bold;text-align: center;margin: 0.5vw 0vw 0.5vw 0vw">Parts</p>
 
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<a class="snd_class" href="https://2018.igem.org/Team:SHSBNU_China/Attributions#Overview">OVERVIEW</a>
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<a class="snd_class" href="https://2018.igem.org/Team:SHSBNU_China/Parts#O">Parts Overview</a>
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<a class="snd_class" href="https://2018.igem.org/Team:SHSBNU_China/Attributions#AD">ATTRIBUTION DETAILS</a>
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<a class="trd_class" href="https://2018.igem.org/Team:SHSBNU_China/Attributions#Q&A">QUESTIONS AND ANSWERS</a>
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<a class="trd_class" href="https://2018.igem.org/Team:SHSBNU_China/Parts#IPP">Improve Previous Parts</a>
 
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<a class="snd_class" href="https://2018.igem.org/Team:SHSBNU_China/Collaborations">SEE COLLABORATIONS</a>
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<h1>Attributions</h1>
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<h1>Parts</h1>
 
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<h2 id="O">I. Overview</h2>
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<h2 id="Overview">I. Overview</h2>
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<p>We have finished constructing all the parts(<a href="http://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2018&group=SHSBNU_China">BBa_K2684000- BBa_K2684006</a>) which have all been sequenced and submitted to the iGEM parts registry</p>
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All of us participated in the experiment and wiki writing parts, and our team members discussed the progress of the experiment and mutually monitored it. All of us have our main works.  
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<td color="#FFFFF0">Name</td>
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<td color="#FFFFF0">Description</td>
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<td color="#FFFFF0">Function</td>
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<td>BBa_K52684000</td>
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<td>CotA laccase of <em>B. subtilis</em></td>
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<td>CotA of <em>B. subtilis</em> is a copper-dependet laccase, which can catalyze the oxidation of board range of synthetic dyes. </td>
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<h2 id="AD">II. Attribution Details</h2>
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<td>BBa_K52684001</td>
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<td>PelB-CotA</td>
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<strong>Zhiran Xie, Chenyu Li, Xuze Shao, Haocong Li, Yijia Yuan, and Yanjun Zhou</strong> participated in Human Hractices. <strong>Haocong Li</strong> photographed, while others kept on asking questions related to our project.
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<td>PelB signal peptide linking with CotA to transfer CotA out of the bacteria. </td>
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                        </p>
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                        <strong>Zhiran Xie, Tieqiong Zhang, Yuanhong Zeng, and Chen Zhang,</strong> all of whom are good at drawing models in computers, collected data and made models
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                        For team wikis, <strong>Chen Zhang</strong> was responsible for editing and coding.
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<td>BBa_K52684002</td>
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                        </p>
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<td>PhoA-CotA</td>
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                        <strong>Wenrui Hu, Fangning Luo, and Chenyu Li, </strong> the art designers of the team, cooperated to make PPT.  
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<td>PhoA signal peptide linking with CotA to transfer CotA out of the bacteria. </td>
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                        </p>
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                        <strong>Tieqiong Zhang and Yanjun Zhou</strong> cooperated to design our safety project and safe shipment.  
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                        For the chart “Photos or Art design”, <strong>Yuanhong Zeng and Xuze Shao</strong> worked for graphs, and <strong>Haocong Li,</strong> the best photographer in Team SHSBNU_China, provided most photos
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<td>BBa_K52684003</td>
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                        </p>
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<td>OmpA-CotA</td>
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<td>OmpA signal peptide linking with CotA to transfer CotA out of the bacteria.</td>
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<td>BBa_K52684004</td>
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<td>SpyCatcher-sfGFP</td>
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<td>SfGFP fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.This part is used to test our immobilization strategy of CotA.</td>
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<td>BBa_K52684005</td>
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<td>SpyCatcher-CotA</td>
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<td>CotA fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.</td>
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<td>BBa_K52684006</td>
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<td>CsgA-SpyTag</td>
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<td>CsgA is a major subunit of the biofilm of E. coli(1)(2). A SpyTag is fused to CsgA so that CotA laccase can be fixed on biofilm through SpyTag-SpyCatcher chemistry. </td>
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<h3 id="IPP">a. Improve Previous Parts</h3>
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<p>We improved part<a href="http://parts.igem.org/Part:BBa_K1583000">BBa_K1583000</a> by adding a sequence of SpyTag in order to fix CotA laccase onto the biofilm.
 
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<h3 id="Q&A">a. Questions and Answers</h3>
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<h2 id="R">II. References</h2>
 
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<strong>Does your institution teach an iGEM or synthetic biology course?</strong>
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(1). Wang, X, et al. “Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control.” <em>Advanced Materials (Deerfield Beach, Fla.).,</em> U.S. National Library of Medicine, Apr. 2018, www.ncbi.nlm.nih.gov/pubmed/29516606.
 
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<li class="content_list">Yes, it is an elective course only for students who have done the “accelerated biology course” of our school. When students have done the accelerated biology course, they will have a test to determine whether they can listen to synthetic biology course and iGEM course in order to broaden their biology knowledge and prepare for the iGEM competition.</li>
 
 
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    <strong>When did you start this course?</strong>
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(2). Chen, Allen Y., et al. “Synthesis and Patterning of Tunable Multiscale Materials with Engineered Cells .” <em>Nature Materials,</em> U.S. National Library of Medicine, May 2014, www.ncbi.nlm.nih.gov/pmc/articles/PMC4063449/.
 
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<li class="content_list">We start our course every year from September till the iGEM competition for next year. Thus, from September to October that year, our school have 2 parallel courses about synthetic biology and iGEM. </li>
 
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<strong>Are the syllabus and course materials freely available online?</strong>
 
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<li class="content_list">Yes, the syllabus and course materials are free to download in our school website. </li>
 
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<strong>When did you start your brainstorming?</strong>
 
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<li class="content_list">We started our brainstorming in the spring of 2018.</li>
 
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<strong>When did you start in the lab?</strong>
 
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    <li class="content_list">We started working in the lab in July 2018.</li>
 
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    <strong>When did you start working on your project?</strong>
 
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    <li class="content_list">We started our project in September 2018.</li>
 
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Revision as of 13:05, 17 October 2018

Parts