Difference between revisions of "Team:Marburg/Draft"

Line 561: Line 561:
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Initially, we carried out foundational experiments to characterize the growth rate, pH, salt and antibiotic tolerance of <i>V. natriegens</i> and sequenced its genome.
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Initially, we carried out foundational experiments to characterize the growth rate, pH, salt and antibiotic tolerance of <i>V. natriegens</i> and sequenced its genome.
 
</td>
 
</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>We performed Cloning in One day: From transformation to isolated plasmids in less than 12 hours!</td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">We performed Cloning in One day: From transformation to isolated plasmids in less than 12 hours!</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Low amounts of DNA could be transformed reliably and we successfully demonstrated challenging clonings such as Gibson Assembly with 5 and Aquacloning with 3 fragments as well as 8 part golden-gate reactions</td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Low amounts of DNA could be transformed reliably and we successfully demonstrated challenging clonings such as Gibson Assembly with 5 and Aquacloning with 3 fragments as well as 8 part golden-gate reactions</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>We designed and constructed the Marburg Collection, a highly flexible golden-gate based toolbox consisting of 123 parts
+
<td class="scrollFade slideInRight" fadeTo="transformIn">We designed and constructed the Marburg Collection, a highly flexible golden-gate based toolbox consisting of 123 parts
 
</td>
 
</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Dozens of test constructs were built and tested to obtain characterization data for all part categories in our toolbox
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Dozens of test constructs were built and tested to obtain characterization data for all part categories in our toolbox
 
</td>
 
</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>This was only possible with our novel experimental and data analysis workflow using platereader measurements
+
<td class="scrollFade slideInRight" fadeTo="transformIn">This was only possible with our novel experimental and data analysis workflow using platereader measurements
 
</td>
 
</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>We successfully demonstrated a genome engineering workflow in <i>V. natriegens</i> to establish three new lab strains for diverse applications.
+
<td class="scrollFade slideInRight" fadeTo="transformIn">We successfully demonstrated a genome engineering workflow in <i>V. natriegens</i> to establish three new lab strains for diverse applications.
 
</td>
 
</td>
<td><div class="checkmark"></div></td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn"><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Subsequently, we were able to use Flp/FRT recombinase system for marker recycling thus allowing additional rounds of genomic modifications</td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Subsequently, we were able to use Flp/FRT recombinase system for marker recycling thus allowing additional rounds of genomic modifications</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>Chromosomal locations suitable for genomic integration were identified and characterized to detect possible fitness effects </td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Chromosomal locations suitable for genomic integration were identified and characterized to detect possible fitness effects </td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
<tr>
 
<tr>
<td>We accelerated metabolic engineering by developing a workflow for rapid pathway assembly and pathway optimization in <i>V. natriegens</i></td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">We accelerated metabolic engineering by developing a workflow for rapid pathway assembly and pathway optimization in <i>V. natriegens</i></td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
                                                 <tr>
 
                                                 <tr>
<td>Model-predicted parts were used to construct a pathway for maximal 3-hydroxypropionate production in <i>V. natriegens</i> and we demonstrated its functionality</td>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Model-predicted parts were used to construct a pathway for maximal 3-hydroxypropionate production in <i>V. natriegens</i> and we demonstrated its functionality</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>
 
</tr>
 
</tr>
 
                                                 <tr>
 
                                                 <tr>
<td>Via mass spectrometry, we detected our pathway product                          3-hydroxypropionic acid in <i>V. natriegens</i>
+
<td class="scrollFade slideInRight" fadeTo="transformIn">Via mass spectrometry, we detected our pathway product                          3-hydroxypropionic acid in <i>V. natriegens</i>
 
</td>
 
</td>
 
<td><div class="checkmark"></div></td>
 
<td><div class="checkmark"></div></td>

Revision as of 22:11, 17 October 2018

Strain Engineering
Part Collection
Metabolic Engineering
Interlab
Accessible Science
B. Marchal