Hpedamallu (Talk | contribs) |
Hpedamallu (Talk | contribs) |
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<p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | ||
<ul> | <ul> | ||
− | <li> | + | <li>Made ampicillin and chloramphenicol [Cam]</li> |
+ | <li>Made kanamycin [Elizabeth]</li> | ||
+ | <li>Made LB+Cam plates [Kyle]</li> | ||
+ | <li>Made overnight DH5α cultures [Diva]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Friday, July 27</u></strong></p> | <p><strong><u>Friday, July 27</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Diva, Elizabeth, Kyle</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Made glycerol stocks of <I>DH5α</I> cells [Diva]</li> |
+ | <li>Made competent <I>DH5α</I> cells [Diva, Elizabeth, Kyle]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Saturday, July 28</u></strong></p> | <p><strong><u>Saturday, July 28</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Elizabeth, Kyle</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Ran competent cell test kit [Elizabeth, Kyle]</li> |
+ | <li>Discussed human practices [Elizabeth, Kyle]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Sunday, July 29</u></strong></p> | <p><strong><u>Sunday, July 29</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Diva, Elizabeth, Kyle</em></p> |
− | + | ||
− | + | ||
<ul> | <ul> | ||
− | <li> | + | <li>Checked plates from competent cell kit [Elizabeth]</li> |
+ | <li>Worked on wiki content for global human practices [Diva, Kyle]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Monday, July 30</u></strong></p> | <p><strong><u>Monday, July 30</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Diva, Elizabeth, Kyle</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Grew an overnight culture of DH5α cells [Diva] |
+ | <li>Transformed NEB competent <I>DH5α</I> cells with pSB1C3 (pre-miniprep) [Elizabeth]</li> | ||
+ | <li>Tested for competent cells [Kyle]</li> | ||
+ | <li>Met with Dr. Brennan to discuss part assembly and competent cell preparation protocol [Diva, Elizabeth, Kyle]</li> | ||
+ | <li>Met with Jeff and other grad students to discuss preventing contamination and improving efficacy of interlab technique [Diva, Elizabeth, Kyle]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Tuesday, July 31</u></strong></p> | <p><strong><u>Tuesday, July 31</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Diva, Elizabeth, Kyle</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Made competent <I>DH5α</I> cells [Kyle, Elizabeth]</li> |
+ | <li>Tested TSS and SOC media pH with glass pH probe [Elizabeth, Diva]</li> | ||
</ul> | </ul> | ||
+ | <center> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/c/c4/T--WashU_StLouis--7311notebook.jpeg width= "30%" /> | ||
+ | <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Cells transformed with pSB1C3 from 7/30</p> | ||
+ | </center> | ||
+ | <center> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/5/55/T--WashU_StLouis--7312notebook.jpeg width= "30%" /> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/5/52/T--WashU_StLouis--7313notebook.jpeg width= "30%" /> | ||
+ | <p style="font-size:14px;font-family: 'Josefin Sans';text-align:center;">Results from competent cell test kit from 7/30</p> | ||
+ | </center> | ||
</div> | </div> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Wednesday, August 1</u></strong></p> | <p><strong><u>Wednesday, August 1</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Diva</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Made LB agar plates [Diva]</li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | ||
<ul> | <ul> | ||
− | <li> | + | <li>Made competent <I>DH5α</I> cells [Cam, Diva]</li> |
+ | <li>Transformed competent cells with RFP Part BBa_I13501 [Kyle, Elizabeth]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | ||
<ul> | <ul> | ||
− | <li> | + | <li>Transformed competent cells with Part BBa_K2663000 [Elizabeth, Kyle]</li> |
+ | <li>Made LB agar + CM plates [Diva, Cam]</li> | ||
+ | <li>Made overnight cultures of cells transformed with RFP Part BBa_I13501 [Cam]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Saturday, August 4</u></strong></p> | <p><strong><u>Saturday, August 4</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Elizabeth, Kyle</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Made LB [Elizabeth, Kyle]</li> |
+ | <li>Made glycerol stocks of overnight cultures [Elizabeth, Kyle]</li> | ||
+ | <li>Plated cells to check for contamination [Elizabeth, Kyle]</li> | ||
+ | <li>Ordered miniprep kit [Elizabeth, Kyle]</li> | ||
+ | <li>Checked plates to ensure lack of contamination [Elizabeth, Kyle]</li> | ||
+ | <li>Plated overnight cultures [Elizabeth, Kyle]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div style="height: 200px; width: 100%;"> | <div style="height: 200px; width: 100%;"> | ||
<p><strong><u>Sunday, August 5</u></strong></p> | <p><strong><u>Sunday, August 5</u></strong></p> | ||
− | <p><em>Present: | + | <p><em>Present: Elizabeth</em></p> |
<ul> | <ul> | ||
− | <li> | + | <li>Made glycerol stocks from overnight cultures [Elizabeth]</li> |
+ | <li>Miniprepped overnight cultures (RFP, ribitol component promoter, and RBS) [Elizabeth]</li> | ||
+ | <li>Autoclaved new tips and microcentrifuge tubes [Elizabeth]</li> | ||
+ | <li>Made a negative control to test for contamination in the culture tubes [Elizabeth]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | ||
<ul> | <ul> | ||
− | <li> | + | <li>Made ampicillin plates [Cam, Diva]</li> |
+ | <li>Measured DNA concentrations using nanodrop [Cam, Diva, Elizabeth, Kyle]</li> | ||
+ | <li>Made more ampicillin, chloramphenicol, and kanamycin [Cam, Diva]</li> | ||
+ | <li>Plated pRSII424 on ampicillin plate [Cam, Diva]</li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | <p><em>Present: Cam, Diva, Elizabeth, Kyle</em></p> | ||
<ul> | <ul> | ||
− | <li> | + | <li>Made an overnight culture of pRSII424 [Cam, Elizabeth, Kyle]</li> |
</ul> | </ul> | ||
</div> | </div> |
Revision as of 15:07, 8 August 2018
June 2018 | ||||||
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