Difference between revisions of "Team:Ecuador"

(Undo revision 193599 by Tefiducky8 (talk))
Line 112: Line 112:
 
       box-shadow: inset -50px -42px 114px -30px rgba(0,0,0,0.75);
 
       box-shadow: inset -50px -42px 114px -30px rgba(0,0,0,0.75);
 
       z-index: 10;
 
       z-index: 10;
 +
 
     }
 
     }
  
Line 242: Line 243:
 
     text-indent: 20px !important;
 
     text-indent: 20px !important;
 
     display: flex !important;
 
     display: flex !important;
−
     background-color: #000 !important;
+
     background-color: #FFF !important;
 
     margin: auto auto !important;
 
     margin: auto auto !important;
 
     align-items: center !important;
 
     align-items: center !important;
Line 333: Line 334:
  
 
     .ecuador_follow_us_icons_container {
 
     .ecuador_follow_us_icons_container {
−
       width: 50%;
+
       width: 43%;
 
       height: 60px;
 
       height: 60px;
 
       position: relative;
 
       position: relative;
Line 339: Line 340:
 
       margin: auto;
 
       margin: auto;
 
       display: flex;
 
       display: flex;
 +
 
     }
 
     }
 +
.ecuador_information_data_container{
 +
width: 50%;
 +
      height: 60px;
 +
      position: relative;
 +
      top: 55px;
 +
      margin: auto;
 +
      display: flex;
 +
}
  
 
     .ecuador_follow_us_icon {
 
     .ecuador_follow_us_icon {
−
       width: 60px;
+
       width: 70px;
 
       height: 60px;
 
       height: 60px;
 
       position: relative;
 
       position: relative;
Line 636: Line 646:
 
       }
 
       }
 
     }
 
     }
 +
 +
.ecuador_sponsors_icons_container {
 +
      width: 90%;
 +
      height: 60px;
 +
      position: relative;
 +
      top: 40px;
 +
      margin: auto;
 +
      display: flex;
 +
    }
 +
.ecuador_sponsors_icon {
 +
      width: 60px;
 +
      height: 60px;
 +
      position: relative;
 +
      margin: auto;
 +
    }
 +
#ing_bio{
 +
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/1/17/T--Ecuador--Logo--BIO.png/902px-T--Ecuador--Logo--BIO.png);
 +
      background-repeat: no-repeat;
 +
      background-position: center;
 +
      background-size: 90%;
 +
      transition: 0.4s;
 +
      border-radius: 5px;
 +
    }
 +
#ing_bio:hover {
 +
      background-size: 93%;
 +
      cursor: pointer;
 +
    }
 +
#espe{
 +
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/d/d2/T--Ecuador--Logo--ESPE.png/1066px-T--Ecuador--Logo--ESPE.png);
 +
      background-repeat: no-repeat;
 +
      background-position: center;
 +
      background-size: 120%;
 +
      transition: 0.4s;
 +
      border-radius: 5px;
 +
    }
 +
#espe:hover {
 +
      background-size: 125%;
 +
      cursor: pointer;
 +
    }
 +
#idgen{
 +
      background-image: url(https://static.igem.org/mediawiki/2018/e/e2/T--Ecuador--Logo--Idgen.png);
 +
      background-repeat: no-repeat;
 +
      background-position: center;
 +
      background-size: 90%;
 +
      transition: 0.4s;
 +
      border-radius: 5px;
 +
    }
 +
#idgen:hover {
 +
      background-size: 93%;
 +
      cursor: pointer;
 +
    }
 +
#tukii{
 +
      background-image: url(https://static.igem.org/mediawiki/2018/thumb/2/26/T--Ecuador--Logo--Tukii.png/1024px-T--Ecuador--Logo--Tukii.png);
 +
      background-repeat: no-repeat;
 +
      background-position: center;
 +
      background-size: 90%;
 +
      transition: 0.4s;
 +
      border-radius: 5px;
 +
    }
 +
#tukii:hover {
 +
      background-size: 93%;
 +
      cursor: pointer;
 +
    }
 +
  
 
   </style>
 
   </style>
Line 853: Line 927:
 
           <div class="ecuador_footer_information_title_container">
 
           <div class="ecuador_footer_information_title_container">
 
             SPONSORS
 
             SPONSORS
 +
<div class="ecuador_sponsors_icons_container">
 +
<div id="espe" class="ecuador_sponsors_icon"></div>
 +
<div id="ing_bio" class="ecuador_sponsors_icon"></div>
 +
              <div id="idgen" class="ecuador_sponsors_icon"></div>
 +
              <div id="tukii" class="ecuador_sponsors_icon"></div>
 +
            </div>
 
           </div>
 
           </div>
 
         </div>
 
         </div>

Revision as of 10:39, 3 October 2018

iGEM ECUADOR
iGEM ECUADOR
What is C-lastin?
Know our idea
About our project
In this project a new biomaterial based on the cross-linking of bacterial cellulose (bacterial celluose, BC) and elastin-like polypeptides (Elastin like polypeptides, ELP) will be developed, for use in biomedical applications. The cross-linking of both materials will be carried out with synthetic biology techniques using the Escherichia coli bacterium as the expression system of the new biomaterial, and as a mediator of the cross-linking to two protein modules of the carbohydrate binding domain (carbohydrate binding domain, CBD) specific for cellulose and one module of bone morphogenic protein 2 (BMP-2).

Bacterial cellulose will be used as a bandage matrix, due its biocompatibility in vivo with the body, moreover its ability to provide an optimal three-dimensional substrate for cell attachment and a microfibrillar structure that provides flexibility, and its high retention capacity of water and gas exchange, which allow it to obtain rapid rates of epithelization and tissue regeneration. CBDs are modules of proteins found in carbohydrate enzymes whose function is to bind to cellulose carbohydrate, allowing the ELP protein to give greater flexibility to the bandage, while the BMP2 protein, which is an inducer of cell differentiation in osteoblasts, will be responsible for reducing the recovery time of the bones.

To achieve the objective, the expression of the cellulose and the fusion protein in two bacteria will be carried out separately. For the synthesis of bacterial cellulose, an E. coli expression system will be used; introducing two plasmids: psb1C3 responsible for cellulose synthesis and psb1A3 responsible for the synthesis of the export system and overproduction. For the production of the fusion protein, plasmid psb1C3 will be used, which will contain the genes for the proteins CBD, ELP and BMP2
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