One of the applications for root-colonising Pseudomonas fluorescens (CT 364) as a chassis organism proposed was to produce a naturally occurring chemical – naringenin. The substance, as demonstrated in our laboratory (link), attracts free-living nitrogen fixing bacteria. Under the right conditions, this would benefit the plant’s nitrogen nourishment and possibly reduce synthetic nitrogen fertilizers usage. Although we already transformed Pseudomonas fluorescens with an operon with genes for naringenin biosynthesis, there is still a long way to test the system on plants. Plants need a lot of time to grow compared to microorganisms. Understanding how the root-colonising bacteria and the nitrogen fixers behave in the soil would be time intensive. To have an early insight and provide visualisations for the public, we developed the microbial community modelling to imitate what’s happening in the soil around the inoculated root.