|
|
| Line 5: |
Line 5: |
| | <h1>Design</h1> | | <h1>Design</h1> |
| | <p> | | <p> |
| − | The HMA3 Sequence is with 2619 bp quite long, so we decided to get it synthesized by IDT.
| |
| − | First option was to use the whole sequence (2631 bp). CAMV35S is used as promoter with 341 bp.
| |
| − | </p>
| |
| − | <p>Our idea is to cut with EcoRI at G/AATTC (above in red). Since this codon happened to be two times in our cDNA sequence, we needed to change this codon two times to prevent a second (and third) cut with EcoRI. You can see that above in blue/red. Then to cut with PstI at CTGCA/G to make sure the sequence. </p>
| |
| | | | |
| − | <p>Second option is to use 4 part fragments, you can see the fractionation in the table below. </p>
| |
| | </div> | | </div> |
| | | | |
| | <div class="column two_thirds_size"> | | <div class="column two_thirds_size"> |
| − | <img src="https://static.igem.org/mediawiki/2018/c/c9/T--HSHL--design-biobrick.png">
| |
| | </div> | | </div> |
| | | | |
