Difference between revisions of "Team:Tongji China/P.A."

Now, we are concerning about the spices and strain of the bacterium involved in our project. We choose Pseudomonas aeruginosa in the strain of PAK-JΔ9 which is an attenuated strain wildly used in the oral method, for it has many advantages varies from efficiency and safety. To be specific, I will introduce the course of searching for this ideal strain step by step.

At the first time, we decided to use Type III secretion system (T3SS) to alert the patient’s immune system, because it seems to be a practical way. We already knew that T3SS is one of the features that belong to the Gram-negative bacteria, and there are significant differences between every species. As a result, some important speices which are wildly used in attenuated vaccine such as Listeria Monocytogenes are out of the range because of its classification. Then, referring to a large number of articles, we discovered that Pseudomonas aeruginosa has a distinctive ability of T3SS among common gram-negative spices (Because its T3SS shows little differences associated with the change of pH value a distinct feature showed in intestinal environment, while others’ items don’t.).

Pseudomonas aeruginosa is one of the ubiquitous gram-negative rods which we can see in many environments. It is also called hospital-acquired infections because it has multidrug-resistance which ensures its survival state. Also, P. aeruginosa is an opportunistic extracellular pathogen to human, because this bacterium is responsible for some diseases such as acute infections with burn wound and its primary hazard is the infections during surgery.

So, such a dangerous pathogen won’t do harm to patients in their oral way? Of course not. Description before this paragraph is for the wild-type P. aeruginosa, while the bacterium we use is modified to ensure its safety. This strain is called PAK-JΔ9, an advance type comparing to PAK-JΔ8, which add the feature that makes it a kind of auxotrophic bacterium. The auxotrophic bacterium, as known as, is not suitable for living in the natural environment nor our intestine. PAK-J Δ9 is coordinate to the fourth most common type of strain, which is the same as the vaccine for human body use.
To know more about the safety of the P.A., you go to the Safety-P.A.

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Go to the Background Neoantigen
Go to the Background T3SS