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Revision as of 16:14, 12 October 2018
Alternative Roots
Influence of Naringenin on Growth Rate of Bacteria in Liquid Media
Principle
Our selected flavonoid, naringenin, is known to possess antimicrobial properties in both gram-positive and gram-negative bacteria [1]. As such, we decided to characterise the effect of naringenin on the growth rate of our bacteria: Azospirillum brasilense, Azorhizobium caulinodans, Herbaspirillum seropedicae, and Escherichia coli. This was done so that we would understand which concentrations of naringenin to use in our chemotaxis assays.
Graphical Overview
INSERT MIC GRAPH HERE
Results
All species successfully grew in the presence of 0-150μM naringenin (Figure 1). However, it was noted that E. coli showed reduced growth rate even at lower concentration of naringenin. When concentration of naringenin exceeded 100μM, there exists greater flux in all species suggesting that naringenin begins to have a greater impact on some, but not all, bacteria. As such, naringenin concentrations of <100μM were used as part of subsequent chemotaxis assays as to not negatively impact bacterial growth.
REFERENCES & Attributions
1. Yang X-e, Wu X, Hao H-l, & He Z-l (2008) Mechanisms and Assessment of Water Eutrophication. Journal of Zhejiang University. Science. B 9(3):197-209.
2. Usman MN, MG; Musa, I (2015) Effect of Three Levels of NPK Fertilizer on Growth Parameters and Yield of Maize-Soybean Intercrop. International Journal of Scientific and Research Publications 5(9).
3. Liu W, et al. (2017) Azorhizobium caulinodans Transmembrane Chemoreceptor TlpA1 Involved in Host Colonization and Nodulation on Roots and Stems. Frontiers in Microbiology 8:1327..
4. Pedrosa FO, et al. (2011) Genome of Herbaspirillum seropedicae Strain SmR1, a Specialized Diazotrophic Endophyte of Tropical Grasses. PLoS Genetics 7(5):e1002064.
Attributions: Connor Trotter