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− | To develop a biosensor, we took two design approaches | + | To develop a biosensor, we took two <a href="https://2018.igem.org/Team:Queens_Canada/Design">design approaches.</a> At the core of both approaches is a modular Nuclear Receptor Ligand binding domain for binding to an analyte. Immediately flanking the ligand binding domain was two linkers of 3-11 amino acids in length, the selection of appropriate linkers was aided by extensive in-silico modelling, and root-mean-square deviation molecular dynamic modelling. On the ends of each linker was our signal transduction system which acts as our method of producing a signal once a ligand has bound the receptor. |
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<img style="width:70%;margin-left:15%" src="https://static.igem.org/mediawiki/2018/2/28/T--Queens_Canada--intein2D.png"/> | <img style="width:70%;margin-left:15%" src="https://static.igem.org/mediawiki/2018/2/28/T--Queens_Canada--intein2D.png"/> |
Revision as of 01:26, 13 October 2018
Laboratory
To develop a biosensor, we took two design approaches. At the core of both approaches is a modular Nuclear Receptor Ligand binding domain for binding to an analyte. Immediately flanking the ligand binding domain was two linkers of 3-11 amino acids in length, the selection of appropriate linkers was aided by extensive in-silico modelling, and root-mean-square deviation molecular dynamic modelling. On the ends of each linker was our signal transduction system which acts as our method of producing a signal once a ligand has bound the receptor.