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<h1>Human Practices</h1>
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At iGEM we believe societal considerations should be upfront and integrated throughout the design and execution of synthetic biology projects. “Human Practices” refers to iGEM teams’ efforts to actively consider how the world affects their work and the work affects the world. Through your Human Practices activities, your team should demonstrate how you have thought carefully and creatively about whether your project is responsible and good for the world. We invite you to explore issues relating (but not limited) to the ethics, safety, security, and sustainability of your project, and to show how this exploration feeds back into your project purpose, design and execution.
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                  <p>For practical projects, especially involving synthetic biology, safety should be attached great importance to. This year, we designed an electricity generation device based on microbial co-culture. In terms of experimental design, experimental operation and so on, we fully considered their safety and implement safety at every step. </p>
  
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                  <h3><strong>1. <span class="red-content">Safe Project Design</span></strong></h3>
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                  <p>Safe strains and safe gene products were selected to complete our project design. The products of the genes transferred into the microorganisms are all determined and do not cause any known diseases to humans, animals and plants. Any microorganisms involved in our project is encapsulate in electricity generation device. So far, the devise can recharge the phone, and in the future, electricity generation based on microbial co-culture may become a new choice of electricity production.</p>
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                  <h4><strong>a. Rhodopseudomonas palustris</strong></h4>
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                  <p>Rhodopseudomonas palustris(DSM5859) is one of the protagonists in our project. It is responsible for converting optical energy into chemical energy with lactic acid producing and secreting lactic acid for the electricity production of Shewanella. Rhodopseudomonas palustris is commonly used in sewage treatment. It is a kind of microorganism being harmless to the human body and environmentally friendly. </p>
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                  <h4><strong>b.Cyanobacteria</strong></h4>
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                  <p>In our project, cyanobacteria are used as a control strain to compare the electricity production efficiency of Shewanella when cyanobacteria and Rhodopseudomonas palustris are respectively co-cultured with Shewanella. The cyanobacteria selected is Synechocystis sp. PCC6803, which is widely used in algae research as a model organism. Because of its long growth cycle, strict growth conditions, and special culture medium, we can control its growth in many ways to avoid pollution.</p>
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                  <h4><strong>c. Shewanella</strong></h4>
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                  <p>Shewanella is the key species for optical energy conversion, which can consume lactate and reduce metal to produce electricity. The Shewanella oneidensis MR-1 we used can be used for wastewater treatment [1]. For the time being, there are no reports of diseases with it. </p>
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                  <h3><strong>2. <span class="red-content">Safe Lab Work</span></strong></h3>
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                  <p>Our team also pay special attention to safety issues during the experimental operation. New members are required to undergo experimental training from Principle Investigators, graduate students and previous team members in our lab before conducting experiments. Experimental training includes standard experiment operations, safety regulations of equipment usage and good practice rules in the polluted area. In addition, undergraduates have to use secondary equipment with company of a graduate student. In our project, microbial co-culture used to generate electricity must be sterilized at 121 ℃ for 25 minutes before disposal. Based on previous experience, we highlight the following points in experimental safety:</p>
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                  <p>1.polluted area must be isolated from the nomal bench. Experimenters are required to wear lab coat and polyvinyl chloride plastic gloves before entering.</p>
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                  <p>2.Toxic reagents must be collected specially.</p>
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                  <p>3.Volatile chemicals involved experiment must be operated in the fuming cupboard.</p>
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                  <p>4.Laboratory appliance must be sterilized before and after use.</p>
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                  <p>5.Maintaing of complex equipment must be in the charge of staff.</p>
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<p>For more information, please see the <a href="https://2018.igem.org/Human_Practices">Human Practices Hub</a>. There you will find:</p>
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                  <h3><strong>3. <span class="red-content">Safe Shipment</span></strong></h3>
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<li> an <a href="https://2018.igem.org/Human_Practices/Introduction">introduction</a> to Human Practices at iGEM </li>
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                  <p>Safety is also important when sending parts to the registry. All materials sent must be frozen dried and the sealed 96-well plate must wrapped with enough bubble paper to prevent breaking. Sometimes the DNA delivery will be checked by customs, so we have prepared safety certification to ensure that the materials are harmless. Packaging and delivery are done in accordance with the requirements of the courier company and iGEM HQ.  
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<li>tips on <a href="https://2018.igem.org/Human_Practices/How_to_Succeed">how to succeed</a> including explanations of judging criteria and advice about how to conduct and document your Human Practices work</li>
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<li>descriptions of <a href="https://2018.igem.org/Human_Practices/Examples">exemplary work</a> to inspire you</li>
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<li>links to helpful <a href="https://2018.igem.org/Human_Practices/Resources">resources</a></li>
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<li>And more! </li>
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<p>On this page, your team should document all of your Human Practices work and activities. You should write about the Human Practices topics you considered in your project, document any activities you conducted to explore these topics (such as engaging with experts and stakeholders), describe why you took a particular approach (including referencing any work you built upon), and explain if and how you integrated takeaways from your Human Practices work back into your project purpose, design and/or execution. </p>
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<p>If your team has gone above and beyond in work related to safety, then you should document this work on your Safety wiki page and provide a description and link on this page. If your team has developed education and public engagement efforts that go beyond a focus on your particular project, and for which would like to nominate your team for the Best Education and Public Engagement Special Prize, you should document this work on your Education and Education wiki page and provide a description and link here. </p>
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<p>The iGEM judges will review this page to assess whether you have met the Silver and/or Gold medal requirements based on the Integrated Human Practices criteria listed below. If you nominate your team for the <a href="https://2018.igem.org/Judging/Awards">Best Integrated Human Practices Special Prize</a> by filling out the corresponding field in the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>, the judges will also review this page to consider your team for that prize.  
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            <h4><strong>Reference </strong></h4>
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          <p> <span>[1] Xiang Xiao, Xiao-Bo Ma, Hang Yuan. Peng-Cheng Liu, Yu-Bin Kei, Hui Xu, Dao-Lin Du, Jian-Fan Sun, Yu-Jie Feng. Photocatalytic properties of zinc sulfide nanocrystals biofabricated by metal-reducing bacterium Shewanella oneidensis MR-1. Journal of Hazardous Materials. 2015, 288: 134~139.</span></p>
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                College of Life Science & Technology<br>
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                Huazhong University of Science and Technology<br>
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                Add: 1037 Luoyu Road, Wuhan, Hubei, China; P.C: 430074<br>
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                Copyright &copy;2018 Huazhong University of Science & Technology. Produced By </span><a href="https://2018.igem.org/Team:HUST-China" target="_blank" style="color:white;">HUST-China</a>
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<h3>Silver Medal Criterion #3</h3>
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<p>Convince the judges you have thought carefully and creatively about whether your work is responsible and good for the world. Document how you have investigated these issues and engaged with your relevant communities, why you chose this approach, and what you have learned. Please note that surveys will not fulfill this criteria unless you follow scientifically valid methods. </p>
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<h3>Gold Medal Criterion #1</h3>
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<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the purpose, design and/or execution of your project. Document how your project has changed based upon your human practices work.
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<h3>Best Integrated Human Practices Special Prize</h3>
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<p>To compete for the Best Integrated Human Practices prize, please describe your work on this page and also fill out the description on the judging form. </p>
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<p>How does your project affect society and how does society influence the direction of your project? How might ethical considerations and stakeholder input guide your project purpose and design and the experiments you conduct in the lab? How does this feedback enter into the process of your work all through the iGEM competition? Document a thoughtful and creative approach to exploring these questions and how your project evolved in the process to compete for this award!</p>
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<p>You must also delete the message box on the top of this page to be eligible for this prize.</p>
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Revision as of 13:34, 13 October 2018

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Reference

[1] Xiang Xiao, Xiao-Bo Ma, Hang Yuan. Peng-Cheng Liu, Yu-Bin Kei, Hui Xu, Dao-Lin Du, Jian-Fan Sun, Yu-Jie Feng. Photocatalytic properties of zinc sulfide nanocrystals biofabricated by metal-reducing bacterium Shewanella oneidensis MR-1. Journal of Hazardous Materials. 2015, 288: 134~139.

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