As Edward Coke already said in the 16 th Century: “Precaution is better than cure”. Safety is
an important factor in research due to the risks it involves. To ensure the individual and
societal safety, a foresighted and responsible work behavior is essential.
Before starting any laboratory work, an instruction including general lab safety, handling of
hazardous material and emergency actions was given. Also we were acquainted with the
regulations relevant for our work. Additionally every member received instructions concerning
the workplace-related specifics like building security and the proper operation of technical
devices.
For personal safety, every member was equipped with lab coats, protective googles and
disposable gloves. When working with harmful materials like UV-light or hazardous
chemicals, the appropriate protective measures have been taken.
In our project no humans or animals were harmed. We worked with S1 (German Biosafety
Level 1) approved organisms such as the E. coli strains DH5alpha and TOP10 as well as
V. natriegens.
Furthermore we investigated if the wildtype V. natriegens is able to survive outside its natural
habitat. This was achieved through exposing the wildtype to different autoclaved
environmental samples.
To ensure that the genetically modified bacteria, we were working with, were not released
into the natural environment nor came in contact with human beings, we strictly adhered to
the applicable S1 regulations S1 regulations and conducted our research in S1 approved laboratories.
All parts involved in our project are on the IGEMs whitelist
(https://2018.igem.org/Safety/White_List) or applications were submitted for these parts.
In order to not favor the spreading of resistance to important drugs, we were not using
antibiotics on the World Health Organization`s list of “Critically Important Antimicrobials".
In our project we fulfil all requirements of the iGEM competition concerning safety and we are confident, that none of the parts and
constructs of our project are posing a risk for the humanity or environment.