| | <p>As Edward Coke already said in the 16 th Century: “Precaution is better than cure”. Safety is<br />an important factor in research due to the risks it involves. To ensure the individual and<br />societal safety, a foresighted and responsible work behavior is essential.<br />Before starting any laboratory work, an instruction including general lab safety, handling of<br />hazardous material and emergency actions was given. Also we were acquainted with the<br />regulations relevant for our work. Additionally every member received instructions concerning<br />the workplace-related specifics like building security and the proper operation of technical<br />devices.<br />For personal safety, every member was equipped with lab coats, protective googles and<br />disposable gloves. When working with harmful materials like UV-light or hazardous<br />chemicals, the appropriate protective measures have been taken.<br />In our project no humans or animals were harmed. We worked with S1 (German Biosafety<br />Level 1) approved organisms such as the <em>E. coli</em> strains DH5alpha and TOP10 as well as <br /><em>V. </em><em>natriegens.</em><br />Furthermore we investigated if the wildtype <em>V. natriegens</em> is able to survive outside its natural<br />habitat. This was achieved through exposing the wildtype to different autoclaved<br />environmental samples.<br />To ensure that the genetically modified bacteria, we were working with, were not released<br />into the natural environment nor came in contact with human beings, we strictly adhered to<br />the applicable S1 regulations <a href="https://www.gesetze-im-internet.de/gentsv/GenTSV.pdf">S1 regulations</a> and conducted our research in S1 approved laboratories.<br />All parts involved in our project are on the IGEMs whitelist<br />(https://2018.igem.org/Safety/White_List) or applications were submitted for these parts.<br />In order to not favor the spreading of resistance to important drugs, we were not using<br />antibiotics on the World Health Organization`s list of <abbr title="WHO list of critically important antimicrobials"><a href="http://apps.who.int/iris/bitstream/handle/10665/255027/9789241512220-eng.pdf;jsessionid=93FD852DF399468F729E535D5A4725E9?sequence=1">“Critically Important Antimicrobials"</a>.</abbr></p> | | <p>As Edward Coke already said in the 16 th Century: “Precaution is better than cure”. Safety is<br />an important factor in research due to the risks it involves. To ensure the individual and<br />societal safety, a foresighted and responsible work behavior is essential.<br />Before starting any laboratory work, an instruction including general lab safety, handling of<br />hazardous material and emergency actions was given. Also we were acquainted with the<br />regulations relevant for our work. Additionally every member received instructions concerning<br />the workplace-related specifics like building security and the proper operation of technical<br />devices.<br />For personal safety, every member was equipped with lab coats, protective googles and<br />disposable gloves. When working with harmful materials like UV-light or hazardous<br />chemicals, the appropriate protective measures have been taken.<br />In our project no humans or animals were harmed. We worked with S1 (German Biosafety<br />Level 1) approved organisms such as the <em>E. coli</em> strains DH5alpha and TOP10 as well as <br /><em>V. </em><em>natriegens.</em><br />Furthermore we investigated if the wildtype <em>V. natriegens</em> is able to survive outside its natural<br />habitat. This was achieved through exposing the wildtype to different autoclaved<br />environmental samples.<br />To ensure that the genetically modified bacteria, we were working with, were not released<br />into the natural environment nor came in contact with human beings, we strictly adhered to<br />the applicable S1 regulations <a href="https://www.gesetze-im-internet.de/gentsv/GenTSV.pdf">S1 regulations</a> and conducted our research in S1 approved laboratories.<br />All parts involved in our project are on the IGEMs whitelist<br />(https://2018.igem.org/Safety/White_List) or applications were submitted for these parts.<br />In order to not favor the spreading of resistance to important drugs, we were not using<br />antibiotics on the World Health Organization`s list of <abbr title="WHO list of critically important antimicrobials"><a href="http://apps.who.int/iris/bitstream/handle/10665/255027/9789241512220-eng.pdf;jsessionid=93FD852DF399468F729E535D5A4725E9?sequence=1">“Critically Important Antimicrobials"</a>.</abbr></p> |
