Difference between revisions of "Team:Newcastle/Protocols"
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<td>Endophyte Development</td> | <td>Endophyte Development</td> | ||
<td><a href="https://static.igem.org/mediawiki/2018/d/d7/T--Newcastle--ReIsolate.docx" style="color:green">View</a></td> | <td><a href="https://static.igem.org/mediawiki/2018/d/d7/T--Newcastle--ReIsolate.docx" style="color:green">View</a></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Electroporation of <I>Pseudomonas</I> sp.</td> | ||
| + | <td>Frank Eardley</td> | ||
| + | <td>Endophyte Development</td> | ||
| + | <td><a href="https://static.igem.org/mediawiki/2018/3/30/T--Newcastle--ElectroporationProtocol.pdf" style="color:green">View</a></td> | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 21:23, 14 October 2018
Experiments
Protocols
EXPERIMENTS
PROTOCOLS
| Protocol | Author | Category | PDF File |
|---|---|---|---|
| Glycerol Stock Preserves | Connor Trotter | General | View |
| Bacterial Isolation from Freeze-Dried Pellet | Connor Trotter | General | View |
| Cell Counting | Connor Trotter | Chemotaxis | View |
| Minimal A Salt Agar Chemotaxis Assay | Connor Trotter | Chemotaxis | View |
| Planting Seeds in Agar-filled Microcentrifuge tubes | Lewis Tomlinson | Endophyte Development | View |
| Preparation and Staining of Microscopy samples | Lewis Tomlinson | Endophyte Development | View |
| Planting Seeds in Agar-filled pipette-tip racks | Lewis Tomlinson | Endophyte Development | View |
| Re-isolating endophytes | Lewis Tomlinson | Endophyte Development | View |
| Electroporation of Pseudomonas sp. | Frank Eardley | Endophyte Development | View |
| Amplification of gblocks and gradient PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Colony PCR of transformed DH5α cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| DPN1 digestion and purification | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gel extraction and purification of the gblocks | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Gibson Assembly of gblocks and pSB1C3 backbone | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Making chemically competent cells | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Miniprep of transformed colonies | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Amplification, Purification and Quantification of the pSB1C3 backbone by PCR | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Quantifying DNA concentration | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Resuspending gblocks and primers | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| SOC media preparation | Heather Bottomley | Naringenin Operon Biosynthesis | View |
| Transformation of the gblocks into pSB1C3 | Heather Bottomley | Naringenin Operon Biosynthesis | View |