Difference between revisions of "Team:HUST-China/Modeling overview"

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Modeling overview

1. Overview

This year, our job can be mainly divided into three parts: Comparison of Rhodopseudomonas and Cyanobacteria, sort of three genes pathway in Rhodopseudomonas, and intelligent device software.

2. Comparison between PSB

In order to produce Lactate， we have two methods, using Cyanobacteria or Rhodopseudomonas. Cyanobacteria is used widely in the laboratory and has many papers to support.，Because it is more efficient and easier to control. But Shewanella is heterotrophic facultative anaerobes, and produces electricity only in anaerobic environment (Justin et al.2009). So we think Rhodopseudomonas may be better to act as an producer so the cycle photophosphorylation does not produce oxygen. But Rhodopseudomonas is not as efficient as Cyanobacteria. So we use computer simulation to find which one is better.

3. Sort of three genes

In Rhodopseudomonas, the sequence of genes in plasmid influences them expression ability. (Shou-Chen.2012) We have three genes to transfer. So we should find out which gene is the most important, and we sort three genes by their importance.

3. Intelligent device software

Using a new multi-culture bacteria system means that we should use a new fermentation strategy. So we use algorithm to know the situation of the system and decide how to control the device, so that the system will work more efficiently and steadily. Reference

Reference

[1] Justin L. Burns, Thomas J. DiChristina. 2009. Anaerobic Respiration of Elemental Sulfur and Thiosulfate by Shewanella oneidensis MR-1 Requires psrA, a Homolog of the phsA Gene of Salmonella enterica Serovar Typhimurium LT2. American Society for Microbiology Journals

[2] 2.Shou-Chen Lo. 2012. Enhancement of Hydrogen Production and Carbon Fixation in Purple Nonsulfur Bacterium by Synthetic Biology. Ph.D. Dissertation

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                    <h3><strong>1. <span class="red-content">Overview </span></strong></h3>
                    <p style="font-size:16px; line-height: 25px;letter-spacing:1px; text-indent:0px;">
-This year, our job can mainly divided into three parts: Comparison of Rhodopseudomonas and Cyanobacteria, sort of three genes pathway in Rhodopseudomonas, and intelligent device software.
+This year, our job can be mainly divided into three parts: Comparison of Rhodopseudomonas and Cyanobacteria, sort of three genes pathway in Rhodopseudomonas, and intelligent device software.
                    </p>
@@ Line 178: / Line 178: @@
                    <h3><strong>2. <span class="red-content">Comparison between PSB</span></strong></h3>
                    <p style="font-size:16px; line-height: 25px;letter-spacing:1px; text-indent:0px;">
-In the past, people use Cyanobacteria to provide energy for electricity producing bacteria. Because it’s efficient and easier to control. But Shewanella species are heterotrophic facultative anaerobes, and produce electricity only in anaerobic environment (Justin et al.2009). So we think Rhodopseudomonas may be better because it’s cycle photophosphorylation doesn’t produce oxygen. But Rhodopseudomonas is not as efficient as Cyanobacteria. So we use computer simulation to find which is better.
+In order to produce Lactate， we have two methods, using Cyanobacteria or Rhodopseudomonas. Cyanobacteria is used widely in the laboratory and has many papers to support.，Because it is more efficient and easier to control. But Shewanella is heterotrophic facultative anaerobes, and produces electricity only in anaerobic environment (Justin et al.2009). So we think Rhodopseudomonas may be better to act as an producer so the cycle photophosphorylation does not produce oxygen. But Rhodopseudomonas is not as efficient as Cyanobacteria. So we use computer simulation to find which one is better.
 </p>
@@ Line 190: / Line 190: @@
                    <h3><strong>3. <span class="red-content">Sort of three genes</span></strong></h3>
                    <p style="font-size:16px; line-height: 25px;letter-spacing:1px; text-indent:0px;">
-In Rhodopseudomonas, the sequence of genes in plasmid influence them expression ability. (Shou-Chen.2012) We have three gene to transfer. So we should find out which gene is most important, and we sort three genes by their importance.
+In Rhodopseudomonas, the sequence of genes in plasmid influences them expression ability. (Shou-Chen.2012) We have three genes to transfer. So we should find out which gene is the most important, and we sort three genes by their importance.
                  </div>
              </div>
@@ Line 201: / Line 201: @@
                    <h3><strong>3. <span class="red-content">Intelligent device software</span></strong></h3>
                    <p style="font-size:16px; line-height: 25px;letter-spacing:1px; text-indent:0px;">
-Using a new multi-culture bacteria system means we should use a new fermentation strategy. So we use algorithm to know the system’s situation and decide how to control the device, letting system work more efficiently and steadily.
+Using a new multi-culture bacteria system means that we should use a new fermentation strategy. So we use algorithm to know the situation of the system and decide how to control the device, so that the system will work more efficiently and steadily.
+Reference
                  </div>
              </div>