(Created page with "<html> <head> <style> body { height: 100%; width: 100%; background-color: transparent !important; } #globalWr...") |
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height: 100%; | height: 100%; | ||
width: 100%; | width: 100%; | ||
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} | } | ||
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margin: 0; | margin: 0; | ||
padding: 0; | padding: 0; | ||
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} | } | ||
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padding: 0; | padding: 0; | ||
margin: -12px 0 0; | margin: -12px 0 0; | ||
− | |||
− | |||
} | } | ||
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} | } | ||
− | + | #sideMenu { | |
− | + | display: none; | |
+ | } | ||
+ | |||
+ | #content, | ||
+ | .vs-content, | ||
+ | .vs-wrapper section { | ||
+ | background-color: transparent !important; | ||
+ | } | ||
+ | |||
+ | .my-backTop:visited { | ||
+ | color: #F8A886 !important; | ||
+ | } | ||
+ | |||
+ | #HQ_page table { | ||
+ | margin: 0 !important; | ||
+ | } | ||
+ | |||
+ | #HQ_page th { | ||
+ | background-color: #BFBFBF !important; | ||
} | } | ||
</style> | </style> | ||
− | + | ||
<link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/common-style?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/common-style?action=raw&ctype=text/css"> | ||
− | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/css- | + | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/collapse?action=raw&ctype=text/css"> |
+ | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/notebook-ME-style?action=raw&ctype=text/css"> | ||
<style> | <style> | ||
− | + | #notebook-content { | |
position: absolute; | position: absolute; | ||
− | + | left: 440px; | |
− | left: | + | top: 110px; |
− | top: | + | width: 900px; |
− | width: | + | |
− | + | ||
− | + | ||
} | } | ||
− | . | + | .table-head { |
− | + | background-color: #BFBFBF; | |
− | + | color: #131313; | |
− | + | } | |
− | + | ||
− | width: | + | .table-body { |
− | + | width: 100%; | |
− | + | } | |
+ | |||
+ | .table-head table, | ||
+ | .table-body table { | ||
+ | width: 100%; | ||
+ | text-align: center; | ||
+ | } | ||
+ | |||
+ | .table-body table tr:nth-child(2n+1) { | ||
+ | background-color: #f2f2f2; | ||
+ | } | ||
+ | |||
+ | .table-body table tr:nth-child(2n) { | ||
+ | background-color: rgb(223, 223, 223); | ||
+ | } | ||
+ | |||
+ | .table-head td, | ||
+ | .table-body td { | ||
+ | height: 42px; | ||
+ | } | ||
+ | |||
+ | .nav-dot-box:hover .nav-dot-card { | ||
+ | display: block; | ||
+ | width: 250px; | ||
+ | right: 160px; | ||
+ | |||
+ | margin-right: 50px; | ||
+ | |||
+ | transition: all .3s ease-in-out .1s; | ||
+ | -webkit-transition: all .3s ease-in-out .1s; | ||
+ | -moz-transition: all .3s ease-in-out .1s; | ||
+ | -ms-transition: all .3s ease-in-out .1s; | ||
+ | -o-transition: all .3s ease-in-out .1s; | ||
} | } | ||
</style> | </style> | ||
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<body id="ibody" class="scoll_dis"> | <body id="ibody" class="scoll_dis"> | ||
− | + | ||
<ul id="left-nav"> | <ul id="left-nav"> | ||
<li> | <li> | ||
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<li> | <li> | ||
− | <a> | + | <a>Human Practices</a> |
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li> | ||
Line 117: | Line 161: | ||
<ul> | <ul> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li> | ||
− | <li><a href="https://2018.igem.org/Team:BIT-China/ | + | <li><a href="https://2018.igem.org/Team:BIT-China/Parts">Parts</a></li> |
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Improve">Improve</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Applied_Design">Applied Design</a></li> | ||
<li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li> | <li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li> | ||
</ul> | </ul> | ||
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<!-- end --> | <!-- end --> | ||
− | <div | + | <div class="Notbook-white-head"></div> |
− | + | ||
− | + | ||
− | <img | + | <div id="ME-content-all" class="ME-container" style="margin-top:calc(25vh - 30px);"> |
+ | <div class="MD-title"> | ||
+ | <div class="ME-title-content"> | ||
+ | <img class="ME-title-img" src="https://static.igem.org/mediawiki/2018/8/84/T--BIT-China--iGEM2018-top_mask.svg"> | ||
+ | <a>What is the point?</a> | ||
+ | <img class="ME-title-img" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-lower_mask.svg"> | ||
+ | </div> | ||
+ | <div class="ME-overview"> | ||
+ | <a class="ME-overview-p"><br> | ||
+ | —————— Just show what things we use in the experiments. | ||
+ | </a> | ||
+ | </div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | + | <div id="ME1" class="cd-section ME-margin-toTitle"> | |
− | + | <div class="ME-title-1"> | |
− | + | <a>Equipments</a> | |
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
+ | <div class="ME-content-all ME-margin-toTitle1 "> | ||
+ | <div id="E1" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-E1" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | Equipments | ||
+ | </h3> | ||
+ | <div id="collapse_body_E1" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Name</th> | ||
+ | <th>Type</th> | ||
+ | <th>Company</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
− | + | <tr> | |
− | + | <td>Transferpettor</td> | |
+ | <td>1000µl 100µl 10µl 2.5µl</td> | ||
+ | <td>Eppendorf</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>ELIASA</td> | |
− | + | <td>Elx808</td> | |
− | + | <td>America Bioteck company</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Electrophresis Apparatus</td> | |
− | + | <td>DYY-6C</td> | |
− | + | <td>Beijing LIUYI bio-technology company</td> | |
− | + | </tr> | |
− | + | ||
− | + | ||
− | + | <tr> | |
− | + | <td>OD Machine</td> | |
+ | <td>UV-2100</td> | ||
+ | <td>UNICO</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Incubator Shaker</td> | |
− | + | <td>IS-RDS3</td> | |
+ | <td>Beijing KECHUANGBAIFANG Technical Co., Ltd</td> | ||
+ | </tr> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | + | <tr> | |
− | + | <td>Electro-heating Standing-temperature Water Bath</td> | |
+ | <td>DK-8D</td> | ||
+ | <td>Shanghai YIHENG Technical Co., Ltd</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Gel-imaging System</td> | |
− | + | <td>GeneGenius</td> | |
+ | <td>SYNGENE</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Blue Rubber Cutting Machine</td> | |
− | + | <td>BD-BGCI</td> | |
− | + | <td>Biofriend</td> | |
− | + | </tr> | |
− | + | ||
− | + | <tr> | |
− | + | <td>Electro-heating Standing-temperature Cultivator</td> | |
+ | <td>SKP-02.420</td> | ||
+ | <td>America HENGFENG company</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>High Speed Freezing Centrifuge</td> | |
− | + | <td>D-37520</td> | |
+ | <td>Germany Thermo company</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Low temperature enzyme even instrument</td> | |
− | + | <td>L1</td> | |
− | + | <td>HEMA</td> | |
− | + | </tr> | |
− | + | ||
− | + | <tr> | |
− | </ | + | <td>Electronic Balance</td> |
+ | <td>FA2104SW</td> | ||
+ | <td>MINQIAO</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>High-Pressure Steam Sterilization Pot</td> | ||
+ | <td>MLS-3750</td> | ||
+ | <td>SANYO</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Laminar Flow Cabinet</td> | ||
+ | <td>SW-CJ-2FD</td> | ||
+ | <td>Taisite</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Microwave</td> | ||
+ | <td>/</td> | ||
+ | <td>Galanz</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Electro-heating Standing-temperature Drum Wind Drying Machine</td> | ||
+ | <td>DHG-9030A</td> | ||
+ | <td>Shanghai YIHENG Technical Co., Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>96 Well Thermal Cycler</td> | ||
+ | <td>/</td> | ||
+ | <td>Gene Company Limited</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>High Speed Micro-Centrifuge</td> | ||
+ | <td>D3024</td> | ||
+ | <td>SCILOGEX</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Refrigerator</td> | ||
+ | <td>BCD-610W</td> | ||
+ | <td>SIEMENS</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Heto Ultra Freeze</td> | ||
+ | <td>/</td> | ||
+ | <td>Thermo</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Ultrapure Water System</td> | ||
+ | <td>/</td> | ||
+ | <td>/</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Ice Machine</td> | ||
+ | <td>SPR-120</td> | ||
+ | <td>SIMAG</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Nano Drop</td> | ||
+ | <td>2000c</td> | ||
+ | <td>America Thermo Scientific company</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Microscope</td> | ||
+ | <td>/</td> | ||
+ | <td>Ruich Allway</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Noise Isolating Tamber</td> | ||
+ | <td>vcx750</td> | ||
+ | <td>Ningbo Scientz Biotechnology CO. , Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Noise Cleaner</td> | ||
+ | <td>KQ-100DE</td> | ||
+ | <td>Kunshang Noise Equipment CO. Ltd.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Real-time fluorescent quantitative PCR</td> | ||
+ | <td>LightCycler 96</td> | ||
+ | <td>Roche</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
− | |||
− | |||
</div> | </div> | ||
− | |||
− | + | <div id="ME2" class="cd-section"> | |
+ | <div class="ME-title-1"> | ||
+ | <a>Reagent & Material</a> | ||
+ | </div> | ||
+ | <div class="ME-content-all ME-margin-toTitle1 "> | ||
+ | <div id="M1" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M1" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 1. Antibiotic | ||
+ | </h3> | ||
+ | <div id="collapse_body_M1" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Antibiotic</th> | ||
+ | <th>Preparation method</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>Ampicillin</td> | ||
+ | <td>Dissolved in distilled water and the final concentration is 100mg/ml</td> | ||
− | + | </tr> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | <tr> | |
− | + | <td>Chloromycetin</td> | |
− | + | <td>Dissolved in absolute ethyl alcohol and final concentration is 25 mg/ml</td> | |
− | + | ||
− | + | </tr> | |
− | + | ||
− | + | ||
− | + | ||
− | + | </tbody> | |
− | + | </table> | |
− | + | </div> | |
− | |||
− | |||
− | |||
− | + | </div> | |
− | + | </div> | |
− | + | </div> | |
− | + | <div id="M2" class="collapseDiv"> | |
− | + | <span class="collapseSpan"></span> | |
− | + | <h3 id="a-nav-dot-box-M2" class="ME-title-content-a"> | |
+ | <span class="collapseCircle"></span> | ||
+ | 2. Maker | ||
+ | </h3> | ||
+ | <div id="collapse_body_M2" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Maker</th> | ||
+ | <th>From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>Trans 2K DNA Maker</td> | ||
+ | <td>Trans Gen Biotech</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Trans 2K plus DNA Maker</td> | |
− | + | <td>Trans Gen Biotech</td> | |
− | + | </tr> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | <tr> | |
− | + | <td>Trans 1KB DNA Maker</td> | |
+ | <td>Trans Gen Biotech</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Trans 2K plusⅡDNA Maker</td> | |
− | + | <td>Trans Gen Biotech</td> | |
− | + | </tr> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | </tbody> | |
− | + | </table> | |
− | + | </div> | |
− | + | ||
− | |||
− | |||
− | |||
− | |||
− | + | </div> | |
− | + | </div> | |
+ | </div> | ||
− | + | <div id="M3" class="collapseDiv"> | |
− | + | <span class="collapseSpan"></span> | |
− | + | <h3 id="a-nav-dot-box-M3" class="ME-title-content-a"> | |
− | + | <span class="collapseCircle"></span> | |
− | + | 3. Buffer | |
− | + | </h3> | |
+ | <div id="collapse_body_M3" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Buffer</th> | ||
+ | <th>From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>6×DNA Loading buffer</td> | ||
+ | <td>Trans Gen Biotech</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>2×PrimeSTARCG buffer</td> | |
− | + | <td>TaKaRa</td> | |
− | + | </tr> | |
− | + | ||
− | + | <tr> | |
− | + | <td>2×Taq PCR Master Mix</td> | |
− | + | <td>Biomed</td> | |
− | + | </tr> | |
− | + | ||
− | + | <tr> | |
− | + | <td>2×ES Taq Master Mix</td> | |
− | + | <td>CWBio</td> | |
− | + | </tr> | |
− | + | ||
− | + | <tr> | |
− | + | <td>2×SYBR Green qPCR Master Mix</td> | |
− | + | <td>TaKaPa</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>10×T4 DNA Ligase Buffer</td> | |
− | + | <td>TaKaRa</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>10×Fast Digest Green Buffer</td> | |
− | + | <td>Scientific</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>10×Fast Digest Buffer</td> | |
− | + | <td>Scientific</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>10×Pfu Buffer</td> | |
− | + | <td>Biomed</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>5×Trans Start FastPfu Buffer</td> | |
− | + | <td>Trans Gen Biotech</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>5×PrimeSTAR Buffer</td> | |
− | + | <td>TaKaRa</td> | |
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>5×FastPfu Fly Buffer</td> | |
− | + | <td>Trans Gen Biotech</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>5×Primrscript RTMaster Mix</td> | |
− | + | <td>TaKaRa</td> | |
− | + | </tr> | |
− | + | </tbody> | |
− | + | </table> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | + | </div> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | <div id="M4" class="collapseDiv"> | |
− | + | <span class="collapseSpan"></span> | |
− | + | <h3 id="a-nav-dot-box-M4" class="ME-title-content-a"> | |
− | + | <span class="collapseCircle"></span> | |
+ | 4. Enzyme | ||
+ | </h3> | ||
+ | <div id="collapse_body_M4" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Enzyme</th> | ||
+ | <th>From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>PrimeSTAR HS DNA polymerase</td> | ||
+ | <td>TaKaRa</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>2×SYBR Green qPCR Master Mix</td> | |
− | + | <td>TaKaPa</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>5×Primrscript RTMaster Mix</td> | |
− | + | <td>TaKaRa</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Pfu DNA polymerase</td> | |
− | + | <td>Biomed</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Trans Start FastPfu DNA polymerase</td> | |
− | + | <td>Trans Gen Biotech</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Trans Start FastPfu Fly DNA polymerase</td> | |
− | + | <td>Trans Gen Biotech</td> | |
− | + | </tr> | |
− | + | ||
− | + | ||
− | + | ||
− | + | <tr> | |
− | + | <td>T4 DNA Ligase</td> | |
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
− | + | <tr> | |
− | + | <td>Fast digest DpnⅠ</td> | |
− | + | <td>Thermo Scientific</td> | |
− | }) | + | </tr> |
+ | |||
+ | <tr> | ||
+ | <td>Fast digest KpnⅠ</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Fast digest NotⅠ</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Fast digest EcoRⅠ</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Fast digest PstⅠ</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Fast digest XbaⅠ</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Fast digest SpeⅠ</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="M5" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M5" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 5. Kit | ||
+ | </h3> | ||
+ | <div id="collapse_body_M5" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Kit</th> | ||
+ | <th>From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>GeneJET Gel Exteaction Kit</td> | ||
+ | <td>Thermo Scientific</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>HiPure Gel Pure DNA Micro Kit</td> | ||
+ | <td>Magen</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>TIAN prep Mini plasmid Kit</td> | ||
+ | <td>TIANGEN BIOTECH (BEIJING) CO.,LTD.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>TIANamp Yeast DNA Kit</td> | ||
+ | <td>TIANGEN BIOTECH (BEIJING) CO.,LTD.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Reactive oxygen species (ROS) test kit (Cat. No.: E004 | ||
+ | Chemiluminescence)</td> | ||
+ | <td>Nanjing Jiancheng Bioengineering Institute</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="M6" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M6" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 6. Agarose Gel Electrophoresis | ||
+ | </h3> | ||
+ | <div id="collapse_body_M6" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Reagent</th> | ||
+ | <th>From / Preparation method</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>Golden View (BIOWEST REGULAR AGAROSE G-10 )</td> | ||
+ | <td>Biomed</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>1×TAE</td> | ||
+ | <td>Diluted with 50×TAE</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>50×TAE</td> | ||
+ | <td>Tris 242g, Na2EDTA·2H2O 37.2g, 57.1ml glacial acetic acid, 1L water</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="M7" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M7" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 7. Culture medium | ||
+ | </h3> | ||
+ | <div id="collapse_body_M7" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Culture medium</th> | ||
+ | <th>Preparation method</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>LB(for E.coli)</td> | ||
+ | <td>10g Tryptone peptone, 5g Yeast extract, 5gNaCl, dissolve in 1.0L water | ||
+ | (add extra 20g agar powder for solid medium )</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>YPD(for yeast)</td> | ||
+ | <td>20g Tryptone peptone, 10g Yeast extract, dissolve in 950ml water. | ||
+ | Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid | ||
+ | medium )</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>SD-His(for yeast)</td> | ||
+ | <td>6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid | ||
+ | mixture, 100mg Leucine, 20mg Tryptophane, 20mg uracil, dissolve in 1.0L | ||
+ | water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for | ||
+ | solid medium )</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>SD-Leu(for yeast)</td> | ||
+ | <td>6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid | ||
+ | mixture, 20mg Histidine, 20mg Tryptophane, 20mg uracil, dissolve in | ||
+ | 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder | ||
+ | for solid medium )</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>SD-Ura(for yeast)</td> | ||
+ | <td>6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid | ||
+ | mixture, 20mg Histidine, 100mg Leucine, 20mg Tryptophane, dissolve in | ||
+ | 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder | ||
+ | for solid medium )</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>SD-Trp(for yeast)</td> | ||
+ | <td>6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid | ||
+ | mixture, 20mg Histidine, 100mg Leucine, 20mg uracil, dissolve in 1.0L | ||
+ | water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for | ||
+ | solid medium )</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="M8" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M8" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 8. Antioxidant | ||
+ | </h3> | ||
+ | <div id="collapse_body_M8" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Antioxidant</th> | ||
+ | <th>From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>L-Ascorbic acid</td> | ||
+ | <td>Tianjin Fuchen Chemical Reagent Factory</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Quercetin</td> | ||
+ | <td>Beijing Dongyan Biotech Co.,Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Catechol</td> | ||
+ | <td>Sinopharm Chemical Reagent Co.,Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Resveratrol</td> | ||
+ | <td>J&K Scientific Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>β-Carotene</td> | ||
+ | <td>J&K Scientific Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>trans-Ferulic acid</td> | ||
+ | <td>J&K Scientific Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Gallic acid</td> | ||
+ | <td>J&K Scientific Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Caffeic acid</td> | ||
+ | <td>J&K Scientific Ltd</td> | ||
+ | </tr> | ||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="M9" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M9" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 9. Bacteria | ||
+ | </h3> | ||
+ | <div id="collapse_body_M9" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Name</th> | ||
+ | <th>From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>TOP10</td> | ||
+ | <td>Biomed</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>DH5α</td> | ||
+ | <td>Biomed</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>CENPK2-1C</td> | ||
+ | <td>iBT SynBios</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="M10" class="collapseDiv"> | ||
+ | <span class="collapseSpan"></span> | ||
+ | <h3 id="a-nav-dot-box-M10" class="ME-title-content-a"> | ||
+ | <span class="collapseCircle"></span> | ||
+ | 10. Other reagents | ||
+ | </h3> | ||
+ | <div id="collapse_body_M10" class="collapse_body"> | ||
+ | <div class="collapse_content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>Name</th> | ||
+ | <th>Preparation method / From</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td>50% Glycerol</td> | ||
+ | <td>Use distilled water to dilute 50ml glycerol twice as much</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>dd H<sub>2</sub>O</td> | ||
+ | <td>/</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>Absolute Ethyl Alcohol</td> | ||
+ | <td>/</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>40% Glucose</td> | ||
+ | <td>20g glycerol dissolved in 90 ml of water.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>PEG (50%w/v)</td> | ||
+ | <td>/</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>1.0M LiAc</td> | ||
+ | <td>6.6g LiAC dissolved in 100 ml of water.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>0.1M LiAc</td> | ||
+ | <td>0.66g LiAC dissolved in 100 ml of water.</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>SS-DNA (10.0mg/ml)</td> | ||
+ | <td>Solarbio</td> | ||
+ | </tr> | ||
+ | |||
+ | <tr> | ||
+ | <td>30% H<sub>2</sub>O<sub>2</sub></td> | ||
+ | <td>Beijing Chemical Works</td> | ||
+ | </tr> | ||
+ | |||
+ | |||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <script src="https://2018.igem.org/Template:BIT-China/js/jquery-min?action=raw&ctype=text/javascript"></script> | ||
+ | <script src="https://2018.igem.org/Template:BIT-China/js/tendina?action=raw&ctype=text/javascript"></script> | ||
+ | <script> | ||
+ | $('#left-nav').tendina({ | ||
+ | animate: true, | ||
+ | speed: 400, | ||
+ | }) | ||
</script> | </script> | ||
+ | <script src="https://2018.igem.org/Template:BIT-China/js/collapse2?action=raw&ctype=text/javascript"></script> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 21:38, 15 October 2018
Equipments
Name | Type | Company |
---|
Transferpettor | 1000µl 100µl 10µl 2.5µl | Eppendorf |
ELIASA | Elx808 | America Bioteck company |
Electrophresis Apparatus | DYY-6C | Beijing LIUYI bio-technology company |
OD Machine | UV-2100 | UNICO |
Incubator Shaker | IS-RDS3 | Beijing KECHUANGBAIFANG Technical Co., Ltd |
Electro-heating Standing-temperature Water Bath | DK-8D | Shanghai YIHENG Technical Co., Ltd |
Gel-imaging System | GeneGenius | SYNGENE |
Blue Rubber Cutting Machine | BD-BGCI | Biofriend |
Electro-heating Standing-temperature Cultivator | SKP-02.420 | America HENGFENG company |
High Speed Freezing Centrifuge | D-37520 | Germany Thermo company |
Low temperature enzyme even instrument | L1 | HEMA |
Electronic Balance | FA2104SW | MINQIAO |
High-Pressure Steam Sterilization Pot | MLS-3750 | SANYO |
Laminar Flow Cabinet | SW-CJ-2FD | Taisite |
Microwave | / | Galanz |
Electro-heating Standing-temperature Drum Wind Drying Machine | DHG-9030A | Shanghai YIHENG Technical Co., Ltd |
96 Well Thermal Cycler | / | Gene Company Limited |
High Speed Micro-Centrifuge | D3024 | SCILOGEX |
Refrigerator | BCD-610W | SIEMENS |
Heto Ultra Freeze | / | Thermo |
Ultrapure Water System | / | / |
Ice Machine | SPR-120 | SIMAG |
Nano Drop | 2000c | America Thermo Scientific company |
Microscope | / | Ruich Allway |
Noise Isolating Tamber | vcx750 | Ningbo Scientz Biotechnology CO. , Ltd |
Noise Cleaner | KQ-100DE | Kunshang Noise Equipment CO. Ltd. |
Real-time fluorescent quantitative PCR | LightCycler 96 | Roche |
1. Antibiotic
Antibiotic | Preparation method |
---|
Ampicillin | Dissolved in distilled water and the final concentration is 100mg/ml |
Chloromycetin | Dissolved in absolute ethyl alcohol and final concentration is 25 mg/ml |
2. Maker
Maker | From |
---|
Trans 2K DNA Maker | Trans Gen Biotech |
Trans 2K plus DNA Maker | Trans Gen Biotech |
Trans 1KB DNA Maker | Trans Gen Biotech |
Trans 2K plusⅡDNA Maker | Trans Gen Biotech |
3. Buffer
Buffer | From |
---|
6×DNA Loading buffer | Trans Gen Biotech |
2×PrimeSTARCG buffer | TaKaRa |
2×Taq PCR Master Mix | Biomed |
2×ES Taq Master Mix | CWBio |
2×SYBR Green qPCR Master Mix | TaKaPa |
10×T4 DNA Ligase Buffer | TaKaRa |
10×Fast Digest Green Buffer | Scientific |
10×Fast Digest Buffer | Scientific |
10×Pfu Buffer | Biomed |
5×Trans Start FastPfu Buffer | Trans Gen Biotech |
5×PrimeSTAR Buffer | TaKaRa |
5×FastPfu Fly Buffer | Trans Gen Biotech |
5×Primrscript RTMaster Mix | TaKaRa |
4. Enzyme
Enzyme | From |
---|
PrimeSTAR HS DNA polymerase | TaKaRa |
2×SYBR Green qPCR Master Mix | TaKaPa |
5×Primrscript RTMaster Mix | TaKaRa |
Pfu DNA polymerase | Biomed |
Trans Start FastPfu DNA polymerase | Trans Gen Biotech |
Trans Start FastPfu Fly DNA polymerase | Trans Gen Biotech |
T4 DNA Ligase | Thermo Scientific |
Fast digest DpnⅠ | Thermo Scientific |
Fast digest KpnⅠ | Thermo Scientific |
Fast digest NotⅠ | Thermo Scientific |
Fast digest EcoRⅠ | Thermo Scientific |
Fast digest PstⅠ | Thermo Scientific |
Fast digest XbaⅠ | Thermo Scientific |
Fast digest SpeⅠ | Thermo Scientific |
5. Kit
Kit | From |
---|
GeneJET Gel Exteaction Kit | Thermo Scientific |
HiPure Gel Pure DNA Micro Kit | Magen |
TIAN prep Mini plasmid Kit | TIANGEN BIOTECH (BEIJING) CO.,LTD. |
TIANamp Yeast DNA Kit | TIANGEN BIOTECH (BEIJING) CO.,LTD. |
Reactive oxygen species (ROS) test kit (Cat. No.: E004 Chemiluminescence) | Nanjing Jiancheng Bioengineering Institute |
6. Agarose Gel Electrophoresis
Reagent | From / Preparation method |
---|
Golden View (BIOWEST REGULAR AGAROSE G-10 ) | Biomed |
1×TAE | Diluted with 50×TAE |
50×TAE | Tris 242g, Na2EDTA·2H2O 37.2g, 57.1ml glacial acetic acid, 1L water |
7. Culture medium
Culture medium | Preparation method |
---|
LB(for E.coli) | 10g Tryptone peptone, 5g Yeast extract, 5gNaCl, dissolve in 1.0L water (add extra 20g agar powder for solid medium ) |
YPD(for yeast) | 20g Tryptone peptone, 10g Yeast extract, dissolve in 950ml water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-His(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 100mg Leucine, 20mg Tryptophane, 20mg uracil, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-Leu(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 20mg Histidine, 20mg Tryptophane, 20mg uracil, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-Ura(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 20mg Histidine, 100mg Leucine, 20mg Tryptophane, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
SD-Trp(for yeast) | 6.7g Yeast Nitrogen Base Without Amino acids, 0.6g Drop-out amino acid mixture, 20mg Histidine, 100mg Leucine, 20mg uracil, dissolve in 1.0L water. Autoclave. Add 50ml 40%Glucose (add extra 20g agar powder for solid medium ) |
8. Antioxidant
Antioxidant | From |
---|
L-Ascorbic acid | Tianjin Fuchen Chemical Reagent Factory |
Quercetin | Beijing Dongyan Biotech Co.,Ltd |
Catechol | Sinopharm Chemical Reagent Co.,Ltd |
Resveratrol | J&K Scientific Ltd |
β-Carotene | J&K Scientific Ltd |
trans-Ferulic acid | J&K Scientific Ltd |
Gallic acid | J&K Scientific Ltd |
Caffeic acid | J&K Scientific Ltd |
9. Bacteria
Name | From |
---|
TOP10 | Biomed |
DH5α | Biomed |
CENPK2-1C | iBT SynBios |
10. Other reagents
Name | Preparation method / From |
---|
50% Glycerol | Use distilled water to dilute 50ml glycerol twice as much |
dd H2O | / |
Absolute Ethyl Alcohol | / |
40% Glucose | 20g glycerol dissolved in 90 ml of water. |
PEG (50%w/v) | / |
1.0M LiAc | 6.6g LiAC dissolved in 100 ml of water. |
0.1M LiAc | 0.66g LiAC dissolved in 100 ml of water. |
SS-DNA (10.0mg/ml) | Solarbio |
30% H2O2 | Beijing Chemical Works |