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+ | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/common-style?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2018.igem.org/Template:BIT-China/css/achieve-common-style?action=raw&ctype=text/css"> | ||
+ | <style> | ||
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− | + | display: block; | |
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+ | } | ||
+ | </style> | ||
+ | <script src="https://2018.igem.org/Template:BIT-China/js/base-loading?action=raw&ctype=text/javascript"></script> | ||
− | + | </head> | |
− | + | ||
− | + | ||
− | + | ||
− | < | + | <body id="ibody" class="scoll_dis"> |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
+ | <ul id="left-nav"> | ||
+ | <li> | ||
+ | <a>PROJECT</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Background">Background</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Description">Description</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Design">Idea & Design</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | < | + | <li> |
+ | <a>EXPERIMENTS</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsRegulator">Regulator</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsInput">Input</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a>MODELING</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Model">Overview</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/FluorescentProbesModel">Fluorescent Probes Model </a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/H2O2DecompositionModel">H<sub>2</sub>O<sub>2</sub> | ||
+ | Decomposition Model</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/roGFP2-Orp1MichaelisEquationModel">roGFP2-Orp1 | ||
+ | Michaelis equations Model</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a>Human Practices</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Human_Practices">Integrated Human Practices</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Public_Engagement">Education & Public Engagement</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Collaborations">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a>NOTEBOOK</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Notebook">Lab Book</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Protocols">Methodology / Protocols</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Equipment">Material & Equipment</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/InterLab">Measurement / InterLab</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | < | + | <li> |
+ | <a>ACHIEVEMENTS</a> | ||
+ | <ul> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/JudgingForm">Judging Form</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Parts">Parts</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Improve">Improve</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Applied_Design">Applied Design</a></li> | ||
+ | <li><a href="https://2018.igem.org/Team:BIT-China/Demonstrate">Demonstrate</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
− | < | + | <li> |
− | < | + | <a>TEAM</a> |
− | <ul> | + | <ul> |
− | <li> | + | <li><a href="https://2018.igem.org/Team:BIT-China/Team">Members</a></li> |
− | < | + | <li><a href="https://2018.igem.org/Team:BIT-China/Attributions">Attributions</a></li> |
− | <li> | + | <li><a href="https://2018.igem.org/Team:BIT-China/Gallery">Gallery</a></li> |
− | < | + | </ul> |
− | <li> | + | </li> |
− | < | + | </ul> |
− | < | + | |
− | </ul> | + | |
− | < | + | <a href="https://2018.igem.org/Team:BIT-China"><img id="imgA" class="imgA-new-pos" src="https://static.igem.org/mediawiki/2018/4/46/T--BIT-China--iGEM2018-A_img.png" /></a> |
− | + | <!-- end --> | |
− | </div> | + | <div id="parts-content-all" class="content_container" style="margin-top: calc(25vh - 30px);"> |
+ | <div class="cd-section"> | ||
+ | <div class="PT-title-1"> | ||
+ | <a style="border-bottom: 3px solid #131313;">PARTS</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div id="PT0" class="cd-section" style="margin-top:15px;"> | ||
+ | <div class="PT-content-all"> | ||
+ | <div class="PT-content"> | ||
+ | <p class="PT-content-p"> | ||
+ | In 2018, BIT-China has submitted 16 parts. We divide our parts into 3 group, Regulator, | ||
+ | Feedback and Output. Each group contains some parts with similar function or close relation. | ||
+ | Different groups also connect with each other. | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
− | <div class=" | + | <div id="PT1" class="cd-section"> |
− | <div class=" | + | <div class="PT-title-2 PT-margin-Title2Up"> |
− | + | <a>Regulator</a> | |
+ | </div> | ||
− | <div class=" | + | <div class="PT-content-all"> |
− | < | + | <div class="PT-title-3 PT-margin-toTitle2"> |
+ | <a>Increase ROS Content </a> | ||
+ | </div> | ||
− | <p> | + | <div class="PT-content"> |
+ | <p class="PT-content-p PT-margin-toTitle3"> | ||
+ | BBa_K2765026 and BBa_K2765021 is a collection of <b>regulate components</b> including coding | ||
+ | protein and expression cassette, which can increase the accumulation of intracellular ROS | ||
+ | through overexpressing yon1 induced by galactose. | ||
+ | </p> | ||
+ | </div> | ||
− | </ | + | <div class="PT-title-3 PT-margin-Title3Up"> |
− | < | + | <a>Improve Tolerance</a> |
− | < | + | </div> |
− | </div> | + | |
+ | <div class="PT-content"> | ||
+ | <p class="PT-content-p PT-margin-toTitle3"> | ||
+ | BBa_K2765030 is 1000bp homologous arm of <i>yca1 </i>gene, which can knock out <i>yca1</i> gene | ||
+ | through | ||
+ | homologous recombination so that the yeast tolerance to H<sub>2</sub>O<sub>2</sub> could be | ||
+ | improved. | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="PT2" class="cd-section"> | ||
+ | <div class="PT-title-2 PT-margin-Title2Up"> | ||
+ | <a>Feedback</a> | ||
+ | </div> | ||
+ | |||
+ | <div class="PT-content-all"> | ||
+ | <div class="PT-title-3 PT-margin-Title3Up"> | ||
+ | <a>Control ROS Content</a> | ||
+ | </div> | ||
+ | <div class="PT-content"> | ||
+ | <p class="PT-content-p PT-margin-toTitle3"> | ||
+ | BBa_K2765000~BBa_K2765005 is a collection of <b>feedback components</b> that are downstream | ||
+ | gene | ||
+ | promoter of Yap1. We use them to turn on the expression of dCas9 which can inhibit the | ||
+ | expression of yno1 gene. Considering the application of our system in the future, to realize | ||
+ | different feedback effect, we verified different promoters, glr1, sod2, trr1, trx2 and gsh1, | ||
+ | with different transcription level. | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="PT3" class="cd-section"> | ||
+ | <div class="PT-title-2 PT-margin-Title2Up"> | ||
+ | <a>Output</a> | ||
+ | </div> | ||
+ | <div class="PT-content-all"> | ||
+ | <div class="PT-title-3 PT-margin-Title3Up"> | ||
+ | <a>Detect ROS content</a> | ||
+ | </div> | ||
+ | |||
+ | <div class="PT-content"> | ||
+ | <p class="PT-content-p PT-margin-toTitle3"> | ||
+ | BBa_K2765040~BBa_K2765053 is a collection of <b>output components</b> including promoters, | ||
+ | coding | ||
+ | protein and expression cassette, which can express redox-sensitive green fluorescent protein | ||
+ | roGFP2-Orp1 and display ROS content in cells by its fluorescence ratio. | ||
+ | </p> | ||
+ | |||
+ | <p class="PT-content-p PT-margin-ContentPUp"> | ||
+ | According to this measurement principle, the concentration of whole roGFP2-Orp1 can influence | ||
+ | the final signal output strength, even detect the same sample, so we verified four promoters, | ||
+ | FBA1, TEF2, TEF1 and ENO2, with different transcription level to solve this interfere. | ||
+ | Considering the application of our system in the future, we made this collection and wished | ||
+ | these parts can be used in different antioxidant detection. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="PT-title-3 PT-margin-Title3Up"> | ||
+ | <a>Improve a previous part</a> | ||
+ | </div> | ||
+ | |||
+ | <div class="PT-content"> | ||
+ | <p class="PT-content-p PT-margin-toTitle3"> | ||
+ | We codon optimized Connell team’s previous part BBa_K2296006[8] and obtained our core part | ||
+ | BBa_K2296006, which code for roGFP2-Orp1 protein. Also we got As a result, it is more suitable | ||
+ | to our chassis organisms---yeast and has better expression in yeast. <a style="text-decoration: none;color:#131313;" | ||
+ | href="https://2018.igem.org/Team:BIT-China/Improve">Check | ||
+ | here to know further | ||
+ | information.</a> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="PT4" class="cd-section" style="margin-top: 20px;text-align: center;font-family: 'helveticaregular';color: #131313;"> | ||
+ | <div class="PT-title-2 PT-margin-Title2Up"> | ||
+ | <a>Part Form</a> | ||
+ | </div> | ||
+ | |||
+ | <div class="PT-content-all PT-margin-toTitle2"> | ||
+ | <div class="PT-content"> | ||
+ | <div class="table-head"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width:50px;" /> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <thead> | ||
+ | <tr style="font-size: 20px;"> | ||
+ | <th>No.</th> | ||
+ | <th>Biobrick</th> | ||
+ | <th>Name</th> | ||
+ | <th>Description</th> | ||
+ | <th>Length</th> | ||
+ | </tr> | ||
+ | </thead> | ||
+ | </table> | ||
+ | </div> | ||
+ | <div class="table-body"> | ||
+ | <table> | ||
+ | <colgroup> | ||
+ | <col style="width:50px;" /> | ||
+ | <col style="width: 100px;" /> | ||
+ | <col style="width: 200px;" /> | ||
+ | <col /> | ||
+ | <col style="width: 80px;" /> | ||
+ | </colgroup> | ||
+ | <tbody style="font-size: 16px;"> | ||
+ | <tr> | ||
+ | <td colspan="5" style="font-family:'kg_second_chances_solidRg';">Regulator</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>1</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765021" target="_Blank">BBa_K2765021</a> | ||
+ | </td> | ||
+ | <td>yno1</td> | ||
+ | <td>ROS Content Increaser</td> | ||
+ | <td>1713 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>2</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765026" target="_Blank">BBa_K2765026</a> | ||
+ | </td> | ||
+ | <td>gal1p-yno1-CYC1T</td> | ||
+ | <td>Increase ROS Content </td> | ||
+ | <td>2651 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>3</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765030" target="_Blank">BBa_K2765030</a> | ||
+ | </td> | ||
+ | <td>homologous arm-ura(△yca1)</td> | ||
+ | <td>Knock out the yca1 gene</td> | ||
+ | <td>3038 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="5" style="font-family:'kg_second_chances_solidRg';">Feedback</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>4</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765000" target="_Blank">BBa_K2765000</a> | ||
+ | </td> | ||
+ | <td>gal1p</td> | ||
+ | <td>Turn on the expression of dCas9</td> | ||
+ | <td>332 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>5</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765001" target="_Blank">BBa_K2765001</a> | ||
+ | </td> | ||
+ | <td>sod2p</td> | ||
+ | <td>Turn on the expression of dCas9</td> | ||
+ | <td>526 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>6</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765002" target="_Blank">BBa_K2765002</a> | ||
+ | </td> | ||
+ | <td>trr1p</td> | ||
+ | <td>Turn on the expression of dCas9</td> | ||
+ | <td>960 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>7</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765003" target="_Blank">BBa_K2765003</a> | ||
+ | </td> | ||
+ | <td>trx2p</td> | ||
+ | <td>Turn on the expression of dCas9</td> | ||
+ | <td>283 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>8</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765005" target="_Blank">BBa_K2765005</a> | ||
+ | </td> | ||
+ | <td>gsh1p</td> | ||
+ | <td>Turn on the expression of dCas9</td> | ||
+ | <td>2027 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td colspan="5" style="font-family:'kg_second_chances_solidRg';">Output</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>9</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765040" target="_Blank">BBa_K2765040</a> | ||
+ | </td> | ||
+ | <td>FBA1p</td> | ||
+ | <td>Turn on the expression of roGFP2-Orp1</td> | ||
+ | <td>631 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>10</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765042" target="_Blank">BBa_K2765042</a> | ||
+ | </td> | ||
+ | <td>ENO2p</td> | ||
+ | <td>Turn on the expression of roGFP2-Orp1</td> | ||
+ | <td>512 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>11</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765043" target="_Blank">BBa_K2765043</a> | ||
+ | </td> | ||
+ | <td>roGFP2-Orp1</td> | ||
+ | <td>ROS Content Detector</td> | ||
+ | <td>1317 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>12</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765045" target="_Blank">BBa_K2765045</a> | ||
+ | </td> | ||
+ | <td>TEF1p</td> | ||
+ | <td>Turn on the expression of roGFP2-Orp1</td> | ||
+ | <td>402 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>13</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765050" target="_Blank">BBa_K2765050</a> | ||
+ | </td> | ||
+ | <td>TEF2p-roGFP2-Orp1-CYC1T</td> | ||
+ | <td>Detect ROS Content</td> | ||
+ | <td>1965 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>14</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765051" target="_Blank">BBa_K2765051</a> | ||
+ | </td> | ||
+ | <td>TEF1p-roGFP2-Orp1- CYC1T</td> | ||
+ | <td>Detect ROS Content</td> | ||
+ | <td>1964 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>15</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765052" target="_Blank">BBa_K2765052</a> | ||
+ | </td> | ||
+ | <td>ENO2p-roGFP2-Orp1- CYC1T</td> | ||
+ | <td>Detect ROS Content</td> | ||
+ | <td>2074 bp</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>16</td> | ||
+ | <td> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K2765051" target="_Blank">BBa_K2765051</a> | ||
+ | </td> | ||
+ | <td>FBA1p-roGFP2-Orp1- CYC1T</td> | ||
+ | <td>Detect ROS Content</td> | ||
+ | <td>2193 bp</td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | </div> | ||
+ | <!-- footer start --> | ||
+ | <div class="footer-all" style="margin-top:80px;"> | ||
+ | <div class="footer-left"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/c/ca/T--BIT-China--iGEM2018-sponsor-1.png"> | ||
+ | <img src="https://static.igem.org/mediawiki/2018/0/07/T--BIT-China--iGEM2018-sponsor-2.png"> | ||
+ | </div> | ||
+ | <div class="footer-right"> | ||
+ | <p style="font-family:'kg_second_chances_solidRg';font-size:12px;margin: 0;padding: 0 0 3px;margin-top: 8px;">Contact</p> | ||
+ | <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;">Institute of | ||
+ | Biotransformation and synthetic biosystem School of Chemistry and Chemical Engineering. | ||
+ | </p> | ||
+ | <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;"> | ||
+ | Beijing Institute of Technology | ||
+ | </p> | ||
+ | <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;"> | ||
+ | 100081, Beijing | ||
+ | </p> | ||
+ | <p style="font-size:15px;line-height: 1.5 !important;margin: 0;padding: 0;margin-top: 6px;"> | ||
+ | Email:lichun@bit.edu.cn | ||
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Revision as of 11:34, 16 October 2018
In 2018, BIT-China has submitted 16 parts. We divide our parts into 3 group, Regulator, Feedback and Output. Each group contains some parts with similar function or close relation. Different groups also connect with each other.
BBa_K2765026 and BBa_K2765021 is a collection of regulate components including coding protein and expression cassette, which can increase the accumulation of intracellular ROS through overexpressing yon1 induced by galactose.
BBa_K2765030 is 1000bp homologous arm of yca1 gene, which can knock out yca1 gene through homologous recombination so that the yeast tolerance to H2O2 could be improved.
BBa_K2765000~BBa_K2765005 is a collection of feedback components that are downstream gene promoter of Yap1. We use them to turn on the expression of dCas9 which can inhibit the expression of yno1 gene. Considering the application of our system in the future, to realize different feedback effect, we verified different promoters, glr1, sod2, trr1, trx2 and gsh1, with different transcription level.
BBa_K2765040~BBa_K2765053 is a collection of output components including promoters, coding protein and expression cassette, which can express redox-sensitive green fluorescent protein roGFP2-Orp1 and display ROS content in cells by its fluorescence ratio.
According to this measurement principle, the concentration of whole roGFP2-Orp1 can influence the final signal output strength, even detect the same sample, so we verified four promoters, FBA1, TEF2, TEF1 and ENO2, with different transcription level to solve this interfere. Considering the application of our system in the future, we made this collection and wished these parts can be used in different antioxidant detection.
We codon optimized Connell team’s previous part BBa_K2296006[8] and obtained our core part BBa_K2296006, which code for roGFP2-Orp1 protein. Also we got As a result, it is more suitable to our chassis organisms---yeast and has better expression in yeast. Check here to know further information.
No. | Biobrick | Name | Description | Length |
---|
Regulator | ||||
1 | BBa_K2765021 | yno1 | ROS Content Increaser | 1713 bp |
2 | BBa_K2765026 | gal1p-yno1-CYC1T | Increase ROS Content | 2651 bp |
3 | BBa_K2765030 | homologous arm-ura(△yca1) | Knock out the yca1 gene | 3038 bp |
Feedback | ||||
4 | BBa_K2765000 | gal1p | Turn on the expression of dCas9 | 332 bp |
5 | BBa_K2765001 | sod2p | Turn on the expression of dCas9 | 526 bp |
6 | BBa_K2765002 | trr1p | Turn on the expression of dCas9 | 960 bp |
7 | BBa_K2765003 | trx2p | Turn on the expression of dCas9 | 283 bp |
8 | BBa_K2765005 | gsh1p | Turn on the expression of dCas9 | 2027 bp |
Output | ||||
9 | BBa_K2765040 | FBA1p | Turn on the expression of roGFP2-Orp1 | 631 bp |
10 | BBa_K2765042 | ENO2p | Turn on the expression of roGFP2-Orp1 | 512 bp |
11 | BBa_K2765043 | roGFP2-Orp1 | ROS Content Detector | 1317 bp |
12 | BBa_K2765045 | TEF1p | Turn on the expression of roGFP2-Orp1 | 402 bp |
13 | BBa_K2765050 | TEF2p-roGFP2-Orp1-CYC1T | Detect ROS Content | 1965 bp |
14 | BBa_K2765051 | TEF1p-roGFP2-Orp1- CYC1T | Detect ROS Content | 1964 bp |
15 | BBa_K2765052 | ENO2p-roGFP2-Orp1- CYC1T | Detect ROS Content | 2074 bp |
16 | BBa_K2765051 | FBA1p-roGFP2-Orp1- CYC1T | Detect ROS Content | 2193 bp |