Difference between revisions of "Team:UESTC-China/achievement"

We have registered for iGEM as UESTC-China on Feburary 8th, 2018 and been accepted on April 18th, 2018. We had a great summer and half of semester for our project. We have registered Giant Jamboree and planned for the travel.

We have made Team Wiki, poster and prepared a wonderful presentation.

We have created a page on the team wiki with clear Attribution of each aspect of the project. Click here for attributions.

We have created and documented Part Pages on the Registry for the parts we made. Click here for part.

We have successfully completed the Fifth International InterLab Measurement Study. Also we have uploaded relevant documents of experiment results. Click here for more details about our InterLab.

We have improved the characterization of two existing BioBrick Parts:BBa_K118022 and BBa_K118023 and documented the characterization of these parts on the Main Page of that Part's Registry entry. Click here for parts.

We have accomplished and submitted the Judging Form and Safety Form on time.

We have successfully validated three new BioBrick Parts (BBa_K2617000,BBa_K2617006 and BBa_K2617010 )worked as expected through experiment. And We have submitted 17 samples of new Parts to the Registry and Documented the experimental characterization of these parts on the Main Page of that Part's Registry entry. Click here for parts.

We have mentored 2 new registered team, the CDHSU-CHINA and UESTC-software, as well as receiving help from SCU-China and Jiangnan on InterLab and chassis organism for butanol fermentation. We also collaborated with NCKU-Tainan and came up with a great idea of combining our project and cooperated with BNU-China and NEFU_China to improve our work. Please read the details on the Collaboration.

We have thought carefully and creatively about whether our work is responsible and good for the world. We have documented on our team wiki how we have investigated these issues and engaged with our relevant communities, why we chose this approach, and what we have learned. Besides, our surveys followed scientifically valid methods which fulfilled this criteria. Click here for more details about our Human Practice.

We conducted a survey for both farmers and other citizens to get their views of the current treatment of straw and clean energy. We found that the phenomenon of burning straw still exists and utilization rate of it is particularly low. Besides, citizen's attitude toward clean energy is friendly but they are still not willing to use it. After understanding the basic situation of straw treatment and clean energy, we have communicated with professionals from Biogas Institute of Ministry of Agriculture and Rural Affairs to understand the current research progress in this field so as to improve our design. We got more information about obstacle in current research of reusing straw, as well as analysis of advantages and improvement in our project. After those above, we consulted biologist in our project to further optimize the pathway and experiment design in a more professional perspective. Click here for more details about our Human Practice.

We have constructed a BBa_K2617017 parts as the improvement of BBa_J23100 . Click here for more details about our improve part.

We designed the experiment scientifically, and obtained the output equation of the product by using the experimental data. The optimal fermentation conditions and maximum yield were obtained, and the validity of the optimal fermentation conditions was verified by experiments. Molecular dynamics models of butanol and hydrogen production systems were also established.

Molecular dynamics models of butanol and hydrogen production systems were also established. For the butanol production system, we find the reaction speed limiting step and the sensitive parameters. For the hydrogen production system, it is theoretically proved that the hydrogen production of the constructed Escherichia coli is relatively high. Click here for more details about our modeling.

We have proved that the straw can be degraded by using of our energy conversion system. Besides, butanol and hydrogen has also been detected successfully. Click here for more details about our demonstrate.

UESTC-China is focusing on where is energy coming from and where is it going to. Our new energy is born from straw, which is producing about one billion tons every year but is seldom used with high efficiency because of its complex structure. Here we use one synthesized multi-functional plasmid which can not only express bifunctional enzymes Xyn10D-Fae1A to destroy the straw structure instead of the acid pretreatment, but also can produce the cellulose degrading enzyme to finally transform the straw to the glucose. Following this step, the other two synthesized plasmids are separately used to transform glucose to butanol and hydrogen. These target products have been successfully detected using MS-GS methods. In summary, we have successfully designed an energy conversion system which can degrade straw into butanol and hydrogen. Click here for our demonstrate.

To establish a bond which connect synthetic biology and public, we devoted ourselves vigorously to public education. We hosted “Gene Go”, a giant public education event which drove the public participation in synthetic biology. We promote iGEM in a more innovative way, such as DIY classroom and stage play adapted from our project. We also open up “iGEM Classroom” for teenagers which contains experiment practice and iGEM classes both online and offline. It impressed them deeply and even embraved them forming their own iGEM team. Furthermore, we created a series of edutainment product such as Crazy Lab (a MOBA game), Gene Card Online, Plasmid Rubik and E.mocoli (online emoticon) to bring more fun in education. Above all, what we created was not only an edutainment product, but also a carrier of iGEM and synthetic biology which output influence no matter where it is.Read more about Education & Public Engagement.

Our team should be in consideration for this award for embodying the value of continuous interplay between theory and experiment.

1. In order to find the best fermentation conditions, we designed the experiment and used the experimental data to produce butanol function. The optimal fermentation conditions and maximum yield were obtained for the optimal solution of the function. Then we experimented it again under the best fermentation conditions, and the results showed that the yield was obviously increased. This reflects the guidance of the model to the experiment and experimental support to the model.

2. We established a molecular dynamics model for biochemical reactions. For the butanol production system, we find the reaction speed limiting step and the sensitive parameters. For the hydrogen production system, we have theoretically proved that the hydrogen production of the constructed Escherichia coli is relatively high. This theoretically demonstrates the feasibility of the project.Click here for more details about our Modeling.

We conduct a survey for both farmers and citizens to get a view of current treatment of straw and clean energy. We found that phenomenon of burning straw still exist and utilization rate of it is particularly low. Besides, citizen’s attitude toward clean energy is friendly but they are still not willing to use. After mastering the basic situation of straw treatment and clean energy, we have communicate with Biogas Institute of Ministry of Agriculture and Rural Affairs to understand the current research progress in this field so as to improve our design. We get more information about obstacle in current research of reusing straw, as well as analysis of advantages and improvement in our project. After those above, we consult biologist of our project for further optimization in pathway and experiment in a more professional perspective.Click here for more details about our Human Practice.

Gene Card Online is an online board game developed by Team UESTC-China, realizing the organic combination of synthetic biology and board game. The most basic elements of synthetic biology are clearly delivered to people under the condition of victory, which is the combination of restriction enzyme, DNA ligase, vector, target gene, bacteria and PCR, and they can understand what synthetic biology is in the process of enjoying the game. This fills the blank of Software Tool about promoting synthetic biology. Putting a board game online can further expand the impact of the our project to drive the development of the project itself. It can be transmitted any place at any time in the world that has access to Internet. It can not only help iGEMer around the world to promote iGEM activities, but also help the contributors of synthetic biology to popularize synthetic biology more vividly.Click here for our software.

Xyn10D-Fae1A is a new bifunctional enzyme, which has the activity of ferulic esterase and xylanase and could directly degrade straw without pretreatment. In detail, Feruloyl esterase can hydrolyze ferulic acid ester groups, which are responsible for attaching in complex cellular cell wall structures. Xylanase can hydrolyze plant cell wall component xylan. It can cut the β-1,4 glycosidic bond between the xylose residues in the xylan backbone.Click here for our New Basic Part.