Beyond the lab program, iGEM (International Genetically Engineered Machine) is a synthetic biology competition dedicated to spread all around the world creativity and engineering possibilities that biology can offer. Education and public engagement embodies this policy and encourages us to share knowledge amongst people. To achieve this goal, scientific education and participation are the best ways to raise people's awareness and make the public approach to sciences. That is what we tried to accomplish through different science popularization activities and contributions.

Laboratory Open Day: Bacterial Painting

School of Life Science and Technology, Xi'an Jiaotong University

For our project we launched a science camp on synthetic biology. One of the most interesting activities was Bacterial Painting. We used solid-state medium as the "paper" to paint on, using heat-activated, IPTG-activated expression of RFP, GFP, and BFP as luminescent proteins. The school professors were invited to lecture on aseptic handling of microbes before the event began. In this way, we have shown the infinite possibilities and fun of IGEM competition and synthetic biology to the students in the lower grades. It allowed them to communicate and ask questions in biology and jobs. We also initiated remote interactions with TUST's IGEMers to facilitate communications between teams.

Teaching in Mawu Primary School

Mawu Primary School, Chang'an District, Xi'an

To make children familiar with the field of synthetic biology, we volunteered to go to Mawu primary school for a simple tutorial. Bacteria model were drawn to let them understand that small bacteria cells are perfect micro factories for a variety of tasks. We did successfully achieve our goal by involving students actively to participate in our tutorial. We taught them some biological basics and our mind is expanded by bringing in new ideas from the students. The road in IGEM will definitely go wider and wider.

We collected a total of 1121 questionnaires. Diabetes is becoming quite commonly in young people, they are more adaptable to online questionnaires, so most of the people we surveyed are young people. Older people's knowledge of diabetes and its prevention are mostly derived from related young people. The survey of mostly young people will thus not affect the accuracy of our findings.

we found from the survey that people shop subjectively without choosing sweeteners, and sugary food with limited energy is very popular. Although D-psicose is a kind of natural sugar, there were less people knowing it. D-psicose is a natural sugar with a sweetness of 70%-80% to that of sucrose but very low in energy, which is good to diabetes. So, whether people will try D-psicose when adding it to the food, more than half of the interviewees chose to give it a try. We believe that the outlook for D-psicose in food will be very promising.

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1. 1. Here are some sweeteners, which one are you familiar with?

   

2. 2. Will you notice the sweetener marked on the package, when you buy food?

   

3. 3. Do you think the number of sweeteners is higher in cheaper food than that in the same kind?

   

4. 4. Here are some common foods, which one do you think have a lot of sweetener?

   

5. 5. Do you know any alternatives to white sugar?

   

6. 6. Would you like to buy some sugar with low energy and good taste to replace white sugar even though it is more expensive?

   

7. 7. Would you mind adding sweeteners to the food?

   

8. 8. Do you think sweeteners are harmful to us?

   

9. 9. Do you know the difference between real sugar and sugar substitute?

   

10. 10. Do you know whether sugar substitute can be added into food separately?

    

11. 11. Here are several possible reasons which cause sweetener harmful to us. Which are plausible?

    

12. 12. Here are some sugar and sweeteners, Which one can be eaten by diabetic patients?

    

13. 13. D-psicose is a nature sugar with low energy and good taste. Would you like to add it into your food or drink?

    

Our project is based on a directed evolution in D-psicose synthesis pathway. Compared to traditional chemical and biosynthesis methods, our method has the advantages of high yield, low energy consumption and low carbon emissions. By introducing the synthetic ideas of our projects, we fully demonstrated the superiority of our projects and the art of synthetic biology. . Eventually, we got the Excellence Award in the competition of energy conservation and emission reduction.

Public participation is very important given that the iGEM competition is a competition that was born to solve practical problems. We actively joined various communities to involve the public into our project. Social media like Wechat public account, Twitter, and public mailbox are constantly used to communicate with the public and other teams. We also conducted questionnaires for online and offline surveys. In addition, social practice of IGEM competition is including offering lectures on synthetic biology, visiting nursing homes, and handing out health manuals.