Difference between revisions of "Team:UESTC-China/Parts"

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             This year, we conducted a series of experiment for the two basic parts, BBa_K118022 and BBa_K118023. We validated BBa_K118022 and BBa_K118023 was able to work in <i>E. coli</i>. In addition we used filter paper assay, CMC enzyme activity assay and Congo Red assay for the two enzymes activities.
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             This year, we conducted a series of experiment for the two basic parts, BBa_K118022 and BBa_K118023. We validated that BBa_K118022 and BBa_K118023 can work successfully in <i>E. coli</i>. In addition we used filter paper assay, CMC enzyme activity assay and Congo Red assay for the two enzymes activities.
 
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Revision as of 06:34, 17 October 2018

team

  • Basic parts
    Part number Name Type Function Length
    BBa_K2617000 Xyn10D-fae1A Coding Bifunctional xylanase/ferulic acid esterase 2181bp
    BBa_K2617001 Xyl3A Coding β-D-glucosidase 2586bp
    BBa_K2617004 FhlA Coding Enterobacter cloacae Fe-hydrogenas FhlA 2128bp
    BBa_K2617005 HydA Coding Hydrogen enzyme 444bp
    BBa_K2617006 GroEL Coding Chaperone protein 1681bp
    BBa_K2617007 GroES Coding Chaperone protein 337bp
    BBa_K2617008 Ter Coding Trans-2-enoyl-CoA reductase 1193bp
    BBa_K2617009 Fdh Coding Formate dehydrogenase 1095bp
    BBa_K2617010 Fre_adhE Translational units The fermentation control element of E. coli 460bp
    BBa_K2617011Fre_ackA Translational units The fermentation control element of E. coli 300bp
    BBa_K2617012 AtoB Coding Acetyl-CoA acetyltransferase 1185bp
    BBa_K2617013 AdhE2 Coding Bifunctional acetaldehyde-CoA/alcohol dehydrogenase 2577bp
    BBa_K2617014 Crt Coding 3-hydroxybutyryl-CoA dehydratase Crt 786bp
    BBa_K2617015 Hbd Coding 3-hydroxybutyryl-CoA dehydrogenase 849bp
  • Parts contribution
    This year, we conducted a series of experiment for the two basic parts, BBa_K118022 and BBa_K118023. We validated that BBa_K118022 and BBa_K118023 can work successfully in E. coli. In addition we used filter paper assay, CMC enzyme activity assay and Congo Red assay for the two enzymes activities.
    Part numberSource
    BBa_K118023iGEM08_Edinburgh
    BBa_K118022iGEM08_Edinburgh
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