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− | This year, we conducted a series of experiment for the two basic parts, BBa_K118022 and BBa_K118023. We validated BBa_K118022 and BBa_K118023 | + | This year, we conducted a series of experiment for the two basic parts, BBa_K118022 and BBa_K118023. We validated that BBa_K118022 and BBa_K118023 can work successfully in <i>E. coli</i>. In addition we used filter paper assay, CMC enzyme activity assay and Congo Red assay for the two enzymes activities. |
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Revision as of 06:34, 17 October 2018
BASIC PART
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Basic parts
Part number Name Type Function Length BBa_K2617000 Xyn10D-fae1A Coding Bifunctional xylanase/ferulic acid esterase 2181bp BBa_K2617001 Xyl3A Coding β-D-glucosidase 2586bp BBa_K2617004 FhlA Coding Enterobacter cloacae Fe-hydrogenas FhlA 2128bp BBa_K2617005 HydA Coding Hydrogen enzyme 444bp BBa_K2617006 GroEL Coding Chaperone protein 1681bp BBa_K2617007 GroES Coding Chaperone protein 337bp BBa_K2617008 Ter Coding Trans-2-enoyl-CoA reductase 1193bp BBa_K2617009 Fdh Coding Formate dehydrogenase 1095bp BBa_K2617010 Fre_adhE Translational units The fermentation control element of E. coli 460bp BBa_K2617011 Fre_ackA Translational units The fermentation control element of E. coli 300bp BBa_K2617012 AtoB Coding Acetyl-CoA acetyltransferase 1185bp BBa_K2617013 AdhE2 Coding Bifunctional acetaldehyde-CoA/alcohol dehydrogenase 2577bp BBa_K2617014 Crt Coding 3-hydroxybutyryl-CoA dehydratase Crt 786bp BBa_K2617015 Hbd Coding 3-hydroxybutyryl-CoA dehydrogenase 849bp -
Parts contributionThis year, we conducted a series of experiment for the two basic parts, BBa_K118022 and BBa_K118023. We validated that BBa_K118022 and BBa_K118023 can work successfully in E. coli. In addition we used filter paper assay, CMC enzyme activity assay and Congo Red assay for the two enzymes activities.
Part number Source BBa_K118023 iGEM08_Edinburgh BBa_K118022 iGEM08_Edinburgh
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