Difference between revisions of "Team:CPU CHINA/Experiments"
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| − | <center><img src="https://static.igem.org/mediawiki/2018/b/bb/T--CPU_CHINA--Experiment-2.png"></center> | + | <center><a href="javascript:void(0)" onclick(showExperiment(6))><img src="https://static.igem.org/mediawiki/2018/b/bb/T--CPU_CHINA--Experiment-2.png"></a></center> |
<center> | <center> | ||
<div> | <div> | ||
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"<h4>HepG2 cells were transfected with 1ug of pGL3-htert, pGL3-hulc and pGL3-control. HepG2 cells were cultured in DEME medium containing 10% heat inactivated bovine serum at 37 ℃ and 5% CO2 constant temperature incubator. HepG2 cells were seeded in 96-well plate according to 2* 10<sup>5</sup>/ well, and cultured in non-antibiotic culture medium for 24 hours, then transfected with liposome (Lipo fectamin3000), and transfected with 3 holes per plasmid. The transfection medium was replaced after 4 to 6 h, and the DEME culture medium containing 10% calf serum was used to continue the culture. After 48 h, the cells were treated with Promege's <a><u>steady-glo Luciferase Assay System kit </u></a>and the activity of <i>Luc</i> was determined.</h4><br>"; | "<h4>HepG2 cells were transfected with 1ug of pGL3-htert, pGL3-hulc and pGL3-control. HepG2 cells were cultured in DEME medium containing 10% heat inactivated bovine serum at 37 ℃ and 5% CO2 constant temperature incubator. HepG2 cells were seeded in 96-well plate according to 2* 10<sup>5</sup>/ well, and cultured in non-antibiotic culture medium for 24 hours, then transfected with liposome (Lipo fectamin3000), and transfected with 3 holes per plasmid. The transfection medium was replaced after 4 to 6 h, and the DEME culture medium containing 10% calf serum was used to continue the culture. After 48 h, the cells were treated with Promege's <a><u>steady-glo Luciferase Assay System kit </u></a>and the activity of <i>Luc</i> was determined.</h4><br>"; | ||
| + | var zhuanran = "<h2>Cell transfection and Screening</h2>"+ | ||
| + | "<h3>1.Cell transfection</h3>"+ | ||
| + | "<h4>HepG2 cells were cultured in DEME medium containing 10% heat inactivated bovine serum at 37 ℃ and 5% CO2 constant temperature incubator. HepG2 cells were seeded in 96-well plate according to 2*10<sup>5</sup>/ well, and cultured in non-antibiotic culture medium for 24 hours, then transfected with liposome (<a><u>Lipofectamine 3000</u></a>) , and transfected with 3 holes per plasmid. The transfection medium was replaced after 4 to 6 h, and the DEME culture medium containing 10% calf serum was used to continue the culture. </h4><br>"+ | ||
| + | |||
| + | "<h3>2.Screening</h3>"+ | ||
| + | "<h4>T--CPU_CHINA--hp-aiyou.webm</h4><br>"+ | ||
| + | |||
| + | "<h2>Luciferase reporter gene assay</h2>"+ | ||
| + | "<h4>pGL3-Basic was used as negative Control, and pGL3-Control was used as positive Control. HepG2 cells were transfected with 1ug of pGL3-htert, pGL3-hulc and pGL3-control(Refer Cell transfection and Screening-Part.1).<h4>"+ | ||
| + | "<h4>After 48 h, the cells were treated with Promege's <a><u>steady-glo Luciferase Assay System kit </u></a>and the activity of <i>Luc</i> was determined.</h4><br>"; | ||
| + | |||
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document.getElementById("showdivContent-txt").innerHTML=qidongzi; | document.getElementById("showdivContent-txt").innerHTML=qidongzi; | ||
document.getElementById("showdivTitle").innerHTML="Promoter"; | document.getElementById("showdivTitle").innerHTML="Promoter"; | ||
| + | }else if(id==6){ | ||
| + | document.getElementById("showdivContent-txt").innerHTML=zhuanran; | ||
| + | document.getElementById("showdivTitle").innerHTML="Transfection"; | ||
} | } | ||
Revision as of 13:37, 17 October 2018
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