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      <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"> We faced some problems during our InterLab characterization, our absorbance at 600nm for silica beads at high dilutions didn't follow the trend. Upon which, we discussed the problem on All India iGEM group, where the teams gave us multiple suggestions and shared their protocols by which we were able to obtain our results as deisred by iGEM. Hence, we would like to express our gratitude towards the Indian iGEM teams.
 
 
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Revision as of 14:23, 17 October 2018

iGEM IIT Delhi

 S. No
Part Name 
BioBrick 
Description 
Part Type 
1
pLTL 
Lac-Tet-Lac Hybrid Promoter
Promoter 
sfGFP_ssrA 
Reporter gene (codon-optimised for E. coli) 
Coding Sequence 
rrnB T1 Terminator + T7Te Terminator 
Double Transcriptional Terminator 
Terminator 
mKate2 
Reporter gene (codon-optimised for E. coli)  
Coding Sequence 
rrnB T1 Terminator 
Transcriptional Terminator
Terminator 
attP TP901-1 
Attachment site for TP901-1 Integrase 
DNA 
BxbI Integrase + ssrA(LVA) deg tag 
CDS for Integrase Bxb1  
Coding Sequence 
attB TP901-1 
Attachment site for TP901-1 Integrase 
DNA 
complement of B0034, RBS on the antisense strand 
Ribosome Binding Site 
RBS 
10 
pLac(lambda) hybrid 
Lac-Lambda Hybrid Promoter 
Promoter
11 
attP Bxb1 
Attachment site for Bxb1 Integrase 
DNA 
12 
attB Bxb1 
Attachment site for Bxb1 Integrase 
DNA 
13 
P7 Promoter 
Constitutive P7 promoter 
Promoter
14
BxbI-Xis  + ssrA(LVA) deg tag 
CDS for Recombinase Directionality Factor for Integrase Bxb1 
Coding Sequence 
15
attB BxbI (reverse orientation) 
Attachment site for Bxb1 Integrase (Reverse) 
DNA 
16

TP901-1 Integrase 

CDS for Integrase TP901-1
Coding Sequence

Contact us

Address

Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi