S. No
Part Name
BioBrick
Description
Part Type
1
pLTL
Lac-Tet-Lac Hybrid Promoter
Promoter
2
sfGFP_ssrA
Reporter gene (codon-optimised for E. coli)
Coding Sequence
3
rrnB T1 Terminator + T7Te Terminator
Double Transcriptional Terminator
Terminator
4
mKate2
Reporter gene (codon-optimised for E. coli)
Coding Sequence
5
rrnB T1 Terminator
Transcriptional Terminator
Terminator
6
attP TP901-1
Attachment site for TP901-1 Integrase
DNA
7
BxbI Integrase + ssrA(LVA) deg tag
CDS for Integrase Bxb1
Coding Sequence
8
attB TP901-1
Attachment site for TP901-1 Integrase
DNA
9
complement of B0034, RBS on the antisense strand
Ribosome Binding Site
RBS
10
pLac(lambda) hybrid
Lac-Lambda Hybrid Promoter
Promoter
11
attP Bxb1
Attachment site for Bxb1 Integrase
DNA
12
attB Bxb1
Attachment site for Bxb1 Integrase
DNA
13
P7 Promoter
Constitutive P7 promoter
Promoter
14
BxbI-Xis + ssrA(LVA) deg tag
CDS for Recombinase Directionality Factor for Integrase Bxb1
Coding Sequence
15
attB BxbI (reverse orientation)
Attachment site for Bxb1 Integrase (Reverse)
DNA
16
CDS for Integrase TP901-1
Coding Sequence