Difference between revisions of "Team:Marburg/Part Collection"

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We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids. 36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk. The Marburg Collection contains 122 parts in total, including: inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.(Range:BBa_K2560001 - BBa_K2560305)
 
We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids. 36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk. The Marburg Collection contains 122 parts in total, including: inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.(Range:BBa_K2560001 - BBa_K2560305)
  
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         <img class="flexAssets" src="https://static.igem.org/mediawiki/parts/2/29/T--Marburg--Cloning_Overview.png">
 
         <img class="flexAssets" src="https://static.igem.org/mediawiki/parts/2/29/T--Marburg--Cloning_Overview.png">
  

Revision as of 14:42, 17 October 2018

Part Collection

Judgin Form Text

We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids. 36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk. The Marburg Collection contains 122 parts in total, including: inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.(Range:BBa_K2560001 - BBa_K2560305)
Figure 1: Hierarchical cloning is facilitated by subsequent Golden Gate reactions.
Basic building blocks like promoters or terminators are stored in level 0 plasmids. Parts from each category of our collection can be chosen to built level 1 plasmids harboring a single transcription unit. Up to five transcription units can be assembled into a level 2 plasmid.
Figure 1: Additional bases and fusion sites ensure correct spacing and allow tags.
Between some parts, additional base pairs were integrated to ensure correct spacing and to maintain the triplet code. We expanded our toolbox by providing N- and C- terminal tags by creating novel fusions and splitting the CDS and terminator part, respectivel

Partcollection

5'Connector
BioBrick Name Plasmid
K2560011 5'Connector Dummy BBa_K2560002 (pSB1C3 derivative)
K2560055 1-6 Connector BBa_K2560002 (pSB1C3 derivative)
K2560065 5'Con1 BBa_K2560002 (pSB1C3 derivative)
K2560066 5'Con2 BBa_K2560002 (pSB1C3 derivative)
K2560067 5'Con3 BBa_K2560002 (pSB1C3 derivative)
K2560066 5'Con4 BBa_K2560002 (pSB1C3 derivative)
K2560069 5'Con5 BBa_K2560002 (pSB1C3 derivative)
K2560075 5'Con1 Short Res BBa_K2560002 (pSB1C3 derivative)
K2560076 5'Con2 Short BBa_K2560002 (pSB1C3 derivative)
K2560077 5'Con3 Short BBa_K2560002 (pSB1C3 derivative)
K2560078 5'Con4 Short BBa_K2560002 (pSB1C3 derivative)
K2560079 5'Con5 Short BBa_K2560002 (pSB1C3 derivative)
K2560095 5'Con1 inv BBa_K2560002 (pSB1C3 derivative)
K2560096 5'Con2 inv BBa_K2560002 (pSB1C3 derivative)
K2560097 5'Con3 inv BBa_K2560002 (pSB1C3 derivative)
K2560098 5'Con4 inv BBa_K2560002 (pSB1C3 derivative)
K2560099 5'Con5 inv BBa_K2560002 (pSB1C3 derivative)
K2560105 5'Con5 inv Ori BBa_K2560002 (pSB1C3 derivative)
K2560107 5'Con1 Res BBa_K2560002 (pSB1C3 derivative)
Promoter
BioBrick Name Plasmid
K2560007 J23100 BBa_K2560002 (pSB1C3 derivative)
K2560009 J23104 BBa_K2560002 (pSB1C3 derivative)
K2560014 J23106 BBa_K2560002 (pSB1C3 derivative)
K2560015 J23115 BBa_K2560002 (pSB1C3 derivative)
K2560017 J23101 BBa_K2560002 (pSB1C3 derivative)
K2560018 J23102 BBa_K2560002 (pSB1C3 derivative)
K2560019 J23103 BBa_K2560002 (pSB1C3 derivative)
K2560020 J23105 BBa_K2560002 (pSB1C3 derivative)
K2560021 J23107 BBa_K2560002 (pSB1C3 derivative)
K2560022 J23108 BBa_K2560002 (pSB1C3 derivative)
K2560023 J23109 BBa_K2560002 (pSB1C3 derivative)
K2560024 J23110 BBa_K2560002 (pSB1C3 derivative)
K2560025 J23111 BBa_K2560002 (pSB1C3 derivative)
K2560026 J23113 BBa_K2560002 (pSB1C3 derivative)
K2560027 J23114 BBa_K2560002 (pSB1C3 derivative)
K2560028 J23116 BBa_K2560002 (pSB1C3 derivative)
K2560029 J23117 BBa_K2560002 (pSB1C3 derivative)
K2560029 J23117 BBa_K2560002 (pSB1C3 derivative)
K2560030 J23118 BBa_K2560002 (pSB1C3 derivative)
K2560031 J23119 BBa_K2560002 (pSB1C3 derivative)
K2560123 pTet BBa_K2560002 (pSB1C3 derivative)
K2560124 pTrc BBa_K2560002 (pSB1C3 derivative)
K2560131 Promoter Dummy BBa_K2560002 (pSB1C3 derivative)
RBS
BioBrick Name Plasmid
K2560008 B0034 BBa_K2560002 (pSB1C3 derivative)
K2560010 B0032 BBa_K2560002 (pSB1C3 derivative)
K2560013 B0031 BBa_K2560002 (pSB1C3 derivative)
K2560016 B0030 BBa_K2560002 (pSB1C3 derivative)
K2560084 RBS Dummy BBa_K2560002 (pSB1C3 derivative)
CDS
BioBrick Name Plasmid
K2560033 E1010 BBa_K2560002 (pSB1C3 derivative)
K2560041 E0030 BBa_K2560002 (pSB1C3 derivative)
K2560042 sfGFP BBa_K2560002 (pSB1C3 derivative)
K2560043 sfGFP Vn BBa_K2560002 (pSB1C3 derivative)
K2560047 Cas9 BBa_K2560002 (pSB1C3 derivative)
K2560051 Lux Operon BBa_K2560002 (pSB1C3 derivative)
K2560054 dCas9 BBa_K2560002 (pSB1C3 derivative)
K2560082 CDS Dummy BBa_K2560002 (pSB1C3 derivative)
K2560114 LacZ Alpha BBa_K2560002 (pSB1C3 derivative)
K2560128 K6600004 BBa_K2560002 (pSB1C3 derivative)
K2560135 SXT Beta BBa_K2560002 (pSB1C3 derivative)
K2560268 tfox Vc BBa_K2560002 (pSB1C3 derivative)
K2560269 tfox Vn BBa_K2560002 (pSB1C3 derivative)
K2560270 SXT BBa_K2560002 (pSB1C3 derivative)
K2560272 flp recombinase BBa_K2560002 (pSB1C3 derivative)
Terminator
BioBrick Name Plasmid
K2560034 B0010 BBa_K2560002 (pSB1C3 derivative)
K2560035 B0015 BBa_K2560002 (pSB1C3 derivative)
K2560081 Terminator Dummy BBa_K2560002 (pSB1C3 derivative)
K2560089 B1002 BBa_K2560002 (pSB1C3 derivative)
K2560091 B0003 BBa_K2560002 (pSB1C3 derivative)
K2560093 B0006 BBa_K2560002 (pSB1C3 derivative)
3'Connector
BioBrick Name Plasmid
K2560012 3'Connector Dummy BBa_K2560002 (pSB1C3 derivative)
K2560070 3'Con1 BBa_K2560002 (pSB1C3 derivative)
K2560071 3'Con2 BBa_K2560002 (pSB1C3 derivative)
K2560072 3'Con3 BBa_K2560002 (pSB1C3 derivative)
K2560073 3'Con4 BBa_K2560002 (pSB1C3 derivative)
K2560080 3'Con5 Ori BBa_K2560002 (pSB1C3 derivative)
K2560100 3'Con1 inv Short BBa_K2560002 (pSB1C3 derivative)
K2560101 3'Con2 inv Short BBa_K2560002 (pSB1C3 derivative)
K2560102 3'Con3 inv Short BBa_K2560002 (pSB1C3 derivative)
K2560103 3'Con4 inv Short BBa_K2560002 (pSB1C3 derivative)
K2560104 3'Con5 inv Short BBa_K2560002 (pSB1C3 derivative)
K2560106 3'Con1 inv Res BBa_K2560002 (pSB1C3 derivative)
K2560108 3'Con1 inv BBa_K2560002 (pSB1C3 derivative)
K2560109 3'Con1 inv Res BBa_K2560002 (pSB1C3 derivative)
K2560110 3'Con2 inv BBa_K2560002 (pSB1C3 derivative)
K2560111 3'Con3 inv BBa_K2560002 (pSB1C3 derivative)
K2560112 3'Con4 inv BBa_K2560002 (pSB1C3 derivative)
K2560113 3'Con5 inv BBa_K2560002 (pSB1C3 derivative)
ORI
BioBrick Name Plasmid
K2560036 ColE1 BBa_K2560002 (pSB1C3 derivative)
K2560037 pMB1 BBa_K2560002 (pSB1C3 derivative)
K2560046 p15A BBa_K2560002 (pSB1C3 derivative)
Antibiotic Resistance
BioBrick Name Plasmid
K2560048 Cam. Res. RFP BBa_K2560002 (pSB1C3 derivative)
K2560056 Kan. Res.(pSB3K3) RFP BBa_K2560002 (pSB1C3 derivative)
K2560057 Kan. Res. (pSB3K3) GFP BBa_K2560002 (pSB1C3 derivative)
K2560058 Tet. Res. (pSB3T5) RFP BBa_K2560002 (pSB1C3 derivative)
K2560059 Tet. Res. (pSB3T5) GFP BBa_K2560002 (pSB1C3 derivative)
K2560125 Carb. Res. RFP BBa_K2560002 (pSB1C3 derivative)
K2560126 Carb. Res. GFP BBa_K2560002 (pSB1C3 derivative)
K2560127 Carb. Res. into BBa_K2560002 BBa_K2560002 (pSB1C3 derivative)
K2560132 Cam. Res. into BBa_K2560002 BBa_K2560002 (pSB1C3 derivative)
K2560133 Kan. Res. into BBa_K2560002 BBa_K2560002 (pSB1C3 derivative)
K2560134 Tet. Res. into BBa_K2560002 BBa_K2560002 (pSB1C3 derivative)
N-Tag
BioBrick Name Plasmid
K2560060 E1010 4x BBa_K2560002 (pSB1C3 derivative)
K2560063 sfGFP 4x BBa_K2560002 (pSB1C3 derivative)
K2560085 His Tag 4x BBa_K2560002 (pSB1C3 derivative)
K2560087 FLAG Tag 4x BBa_K2560002 (pSB1C3 derivative)
K2560129 K660004 4x BBa_K2560002 (pSB1C3 derivative)
K2560257 Streptag 4x BBa_K2560002 (pSB1C3 derivative)
C-Tag
BioBrick Name Plasmid
K2560061 E1010 5a BBa_K2560002 (pSB1C3 derivative)
K2560062 B0015 5a BBa_K2560002 (pSB1C3 derivative)
K2560064 sfGFP 5a BBa_K2560002 (pSB1C3 derivative)
K2560086 His Tag 5a BBa_K2560002 (pSB1C3 derivative)
K2560088 FLAG Tag 5a BBa_K2560002 (pSB1C3 derivative)
K2560090 B1002 5b BBa_K2560002 (pSB1C3 derivative)
K2560092 B1003 5b BBa_K2560002 (pSB1C3 derivative)
K2560094 B1006 5b BBa_K2560002 (pSB1C3 derivative)
K2560117 I11012 5a BBa_K2560002 (pSB1C3 derivative)
K2560118 M0050 5a BBa_K2560002 (pSB1C3 derivative)
K2560119 M0051 5a BBa_K2560002 (pSB1C3 derivative)
K2560130 K660004 5a BBa_K2560002 (pSB1C3 derivative)
K2560258 Streptag 5a BBa_K2560002 (pSB1C3 derivative)
Entry Vector
BioBrick Name Plasmid
K2560001 Entry Vector with RFP Dropout BBa_K2560002 (pSB1C3 derivative)
K2560002 Entry Vector with GFP Dropout BBa_K2560002 (pSB1C3 derivative)
K2560005 Resistance Entry Vector with RFP Dropout Falsch
K2560006 Resistance Entry Vector with GFP Dropout Falsch
K2560305 gRNA Entry Vector with GFP Dropout BBa_K2560002 (pSB1C3 derivative)