Difference between revisions of "Team:Tongji China/Capsule"

Up to now, we’ve successfully obtained transformed P. aeruginosa expressing our antigenic peptides as T3SS effectors. To make our aim bacteria the form more suitable for therapeutic use, we decided to prepare them into freeze-dried powder as filler of capsules. One more thing we must take account of is that since we’re aiming at colorectal cancer (CRC), the drug must be released in the small intestine. So we have to find an enteric capsule shell, which is acid-resistant and merely soluble in the small intestine, to protect our therapeutic bacteria from being destroyed by gastric acid. After consulting Shanghai Roche Pharmaceuticals Ltd. and comprehensive web searching, we finally decided on one product of Suzhou Capsugel ® Ltd., DRcaps^TM. According to the instructions of DRcaps^TM, it bears at least the following merits that suit our project well:

We found that SJTU-BioX-Shanghai was also in search of enteric capsules for their own project, and that they knew well how to make freeze-dried powder. So we communicated to reach an agreement that we would provide them with reliable capsule shells and they help us prepare our bacterium freeze-dried powder in return. On 15th October, we trafficked empty DRcaps^TM capsules and aim bacteria stored in liquid medium to Shanghai Jiaotong University. There, we were glad to see our P. aeruginosa turn into dried, pinky powder. Later, we filled it into empty capsule shells carried with us in biosafety cabinet. We sincerely appreciate iGEMers and teachers of SJTU for their generous help.