Difference between revisions of "Team:BIT-China/Demonstrate"

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         .EXP-Fig img {
 
         .EXP-Fig img {
             width: 500px;
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             width: 580px;
 
         }
 
         }
  
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                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsFeedback">Feedback</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
 
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsOutput">Output</a></li>
                 <li><a href="https://2018.igem.org/Team:BIT-China/ExperimentsInput">Input</a></li>
+
                 <li><a href="https://2018.igem.org/Team:BIT-China/Results">Results</a></li>
 
             </ul>
 
             </ul>
 
         </li>
 
         </li>
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             <div class="PT-content-all">
 
             <div class="PT-content-all">
 
                 <div class="PT-content">
 
                 <div class="PT-content">
                    <p class="PT-content-p">
 
                        Our engineered yeast should finally have the function of monitoring antioxidants in real time.
 
                        To demonstrate this, we integrated the two subsystems together into ∆<i>yca1</i> yeast and had
 
                        series of tests on it, in which the increasing of ROS in yeast can be effectively captured by
 
                        our roGFP2-Orp1 protein.
 
                    </p>
 
 
                    <p class="PT-content-p">
 
                        In order to regulate endogenous ROS, we replaced yeast endogenous YNO1 <b>promotor</b> with a
 
                        galactose-inducible promotor, GAL1-GAL10. We then constructed the plasmid
 
                        pESC-TEF1p-roGFP2-Orp1-CYC1t, a shuttle vector marked with <i>trp1</i>. Cellular redox status
 
                        were monitored by measuring the fluorescence ratio at 488 nm(reduced state) and 405 nm(oxidized
 
                        state). Cells were cultured in SD medium supplemented with 1% galactose at 30 ℃, during which
 
                        GAL1-GAL10 promotor was induced when galactose added. It suggests that overexpression of <i>yno1</i>yno1
 
                        gene will increase cells ROS level and decline the fluorescence ratio at 488 nm and 405 nm,
 
                        which means there are more ROS in our modified strain.
 
                    </p>
 
 
                    <p class="PT-content-p">
 
                        As the results showing, our output subsystem can reflect the accumulation of endogenous ROS
 
                        caused by regulator subsystem(figure). A simple decription.
 
                    </p>
 
 
                    <p class="PT-content-p">
 
                        Since we have proved the feasibility that the antioxidative ability of antioxidant can be
 
                        measured by fluorescence probes(6.3.4). We can easily set up the relationship between our
 
                        system and real antioxidative ability of antioxidant. However, due to time limitation, the
 
                        final results will only be shown during the Giant Jamboree. Our presentation will on Saturday
 
                        3:15 PM - 3:45 PM, Room 312 and our poster will at Zone 4 - #235 and welcome to us to see our
 
                        great work!
 
                    </p>
 
 
                     <figure class="EXP-Fig">
 
                     <figure class="EXP-Fig">
                         <img src="https://static.igem.org/mediawiki/2018/2/2f/T--BIT-China--iGEM2018-ach-demo.png">
+
                         <a href="https://2018.igem.org/Team:BIT-China/Parts" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/a/a1/T--BIT-China--Demonstrate1Parts.png"></a>
                         <figcaption></figcaption>
+
                    </figure>
 +
                    <figure class="EXP-Fig">
 +
                        <a href="https://2018.igem.org/Team:BIT-China/Design" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/c/c5/T--BIT-China--Demonstrate2GeneCircuit.png"></a>
 +
                    </figure>
 +
                    <figure class="EXP-Fig">
 +
                         <a href="https://2018.igem.org/Team:BIT-China/Results" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/0/07/T--BIT-China--Demonstrate3Result.png"></a>
 +
                    </figure>
 +
                    <figure class="EXP-Fig">
 +
                        <a href="https://2018.igem.org/Team:BIT-China/HPOverview" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/4/45/T--BIT-China--Demonstrate4HP.png"></a>
 +
                    </figure>
 +
                    <figure class="EXP-Fig">
 +
                        <a href="https://2018.igem.org/Team:BIT-China/Collaborations" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/d/d9/T--BIT-China--Demonstrate5Collaboration.png"></a>
 +
                    </figure>
 +
                    <figure class="EXP-Fig">
 +
                        <a href="https://2018.igem.org/Team:BIT-China/Model" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/c/c1/T--BIT-China--Demonstrate6Modeling.png"></a>
 +
                    </figure>
 +
                    <figure class="EXP-Fig">
 +
                        <a href="https://2018.igem.org/Team:BIT-China/Attributions" target="_Blank"> <img src="https://static.igem.org/mediawiki/2018/f/fe/T--BIT-China--Demonstrate7Attribution.png"></a>
 
                     </figure>
 
                     </figure>
 
                 </div>
 
                 </div>
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             <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;color:  rgb(161, 161, 161);">
 
             <p style="font-size:15px;margin: 0;padding: 0;margin-top: 6px;color:  rgb(161, 161, 161);">
                 Copyright © 2018 BIT-China
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                 Copyright © 2018 BIT-China
 
             </p>
 
             </p>
 
         </div>
 
         </div>

Revision as of 18:39, 17 October 2018