Difference between revisions of "Team:HSHL/Results"

m
Line 3: Line 3:
  
  
−
<div class="column full_size">
+
<div class="column two_thirds_size">
 +
<a name="top"></a>
 
<h1>Results</h1>
 
<h1>Results</h1>
−
<p>Here you can describe the results of your project and your future plans. </p>
+
<p>The synthesis of the HMA3 gene which was needed for this experiment was done by IDT. A gene with the entire sequence was synthesized as well as a gene that was divided into four fragments.
−
</div>
+
First, Gen and Vector were prepared for ligation by cutting both components using the enzymes EcoRI and PstI. The vector, called pSB1C3 was then dephosphorilised by Shrimp Alkaline Phosphatase and the insert, the gene HMA3, phosphorilised by T4 Polynucleotide Kinase. After that gel electrophoresis and the Wizard SV Gel and PCR Clean- Up System of the company Promega were used to purify both components. Below you can see the result of the gel electrophoresis purification of the four fragments. </p>
  
  
−
<div class="column third_size" >
+
<p>The IDT 2- log DNA ladder was used as a control. The following figure shows the Ladder schematically.</p>
  
−
<h3>What should this page contain?</h3>
 
−
<ul>
 
−
<li> Clearly and objectively describe the results of your work.</li>
 
−
<li> Future plans for the project. </li>
 
−
<li> Considerations for replicating the experiments. </li>
 
−
</ul>
 
−
</div>
 
  
 +
<p>By evaluating the gel electrophoresis results with the help of the ladder, the following quantities results are determined for the four fragments. </p>
  
 +
<table>
 +
<tr>
 +
  <th>Fragment No.</th>
 +
  <th>Length [bp]</th>
 +
</tr>
 +
<tr>
 +
  <td>1</td>
 +
  <td>614</td>
 +
</tr>
 +
<tr>
 +
  <td>2</td>
 +
  <td>593</td>
 +
</tr>
 +
<tr>
 +
  <td>3</td>
 +
  <td>556</td>
 +
</tr>
 +
<tr>
 +
  <td>4</td>
 +
  <td>885</td>
 +
</tr>
 +
</table>
  
  
−
<div class="column two_thirds_size" >
 
−
<h3>Describe what your results mean </h3>
 
−
<ul>
 
−
<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
 
−
<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
 
−
<li> Consider including an analysis summary section to discuss what your results mean. Judges like to read what you think your data means, beyond all the data you have acquired during your project. </li>
 
−
</ul>
 
 
</div>
 
</div>
  
  
−
<div class="clear extra_space"></div>
+
<div class="column third_size">
−
 
+
<center><img src="https://static.igem.org/mediawiki/2018/b/b9/T--HSHL--gelImg.png"> <br>
−
 
+
<p>Result of gel electrophoresis purification of the four fragments</p>
−
 
+
−
<div class="column two_thirds_size" >
+
−
<h3> Project Achievements </h3>
+
−
 
+
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
+
−
 
+
−
<ul>
+
−
<li>A list of linked bullet points of the successful results during your project</li>
+
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
+
−
</ul>
+
  
 +
<img src="https://static.igem.org/mediawiki/2018/7/73/T--HSHL--idt2-logLadder.jpg">
 +
<p>Representation of the IDT 2-log ladder and the length of its bands</p>
 
</div>
 
</div>
−
 
−
 
−
 
−
<div class="column third_size" >
 
−
<div class="highlight decoration_A_full">
 
−
<h3>Inspiration</h3>
 
−
<p>See how other teams presented their results.</p>
 
−
<ul>
 
−
<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
 
−
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
 
−
</ul>
 
−
</div>
 
−
</div>
 
−
 
 
<div class="clear extra_space"></div>
 
<div class="clear extra_space"></div>
 
<div class="line_divider"></div>
 
<div class="line_divider"></div>

Revision as of 20:13, 17 October 2018

HOME
ABOUT US
Team
University
Collaborations & Networking
PROJECT
Description
Design
Experiments
Results
Attributions
PARTS
SAFETY
HUMAN PRACTICES
AWARDS
Applied Design
Entrepreneurship
Hardware
Measurement
Model
Plant
Software
CONTACT & COPYRIGHT
JUDGING FORM ⇗

Results

The synthesis of the HMA3 gene which was needed for this experiment was done by IDT. A gene with the entire sequence was synthesized as well as a gene that was divided into four fragments. First, Gen and Vector were prepared for ligation by cutting both components using the enzymes EcoRI and PstI. The vector, called pSB1C3 was then dephosphorilised by Shrimp Alkaline Phosphatase and the insert, the gene HMA3, phosphorilised by T4 Polynucleotide Kinase. After that gel electrophoresis and the Wizard SV Gel and PCR Clean- Up System of the company Promega were used to purify both components. Below you can see the result of the gel electrophoresis purification of the four fragments.

The IDT 2- log DNA ladder was used as a control. The following figure shows the Ladder schematically.

By evaluating the gel electrophoresis results with the help of the ladder, the following quantities results are determined for the four fragments.

Fragment No. Length [bp]
1 614
2 593
3 556
4 885

Result of gel electrophoresis purification of the four fragments

Representation of the IDT 2-log ladder and the length of its bands

This wiki is designed by HSHL within the iGEM 2018 template.
Instagram | Twitter | University | Fundraiser | E-Mail