Difference between revisions of "Team:Marburg"
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| − | Establishing <i>Vibrio natriegens</i> as the new chassis organism for synthetic biology | + | Establishing <i>Vibrio natriegens</i> as the new chassis organism for synthetic biology. |
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| − | We created the Marburg Collection, a highly flexible golden-gate based cloning toolbox consisting of 123 individual parts. Our novel measurement workflow was applied to obtain highly reproducible data on the behavior of our parts in <i>V. natriegens</i> | + | We created the Marburg Collection, a highly flexible golden-gate based cloning toolbox consisting of 123 individual parts. Our novel measurement workflow was applied to obtain highly reproducible data on the behavior of our parts in <i>V. natriegens</i>. |
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<div id="m_a" class="medal_content"> | <div id="m_a" class="medal_content"> | ||
<h1>Bronze</h1> | <h1>Bronze</h1> | ||
| − | We fulfilled all bronze medal criteria | + | We fulfilled all bronze medal criteria. |
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| − | Our team registered for iGEM, we had a challenging but great iGEM season and we are really looking forward to attend the Giant | + | Our team registered for iGEM, we had a challenging but great iGEM season and we are really looking forward to attend the Giant Jamboree. |
<h2>Deliverables</h2> | <h2>Deliverables</h2> | ||
| − | We already finalized our Wiki and <a href="https://igem.org/2018_Judging_Form?id=2560">Judging Form</a> We will hold our presentation at the Giant Jamboree in Boston and we expect exciting discussions at our poster. | + | We already finalized our Wiki and <a href="https://igem.org/2018_Judging_Form?id=2560">Judging Form</a>. We will hold our presentation at the Giant Jamboree in Boston and we expect exciting discussions at our poster. |
<h2>Attributions</h2> | <h2>Attributions</h2> | ||
Our project was only possible with a great <a href="https://2018.igem.org/Team:Marburg/Team">team</a> and we also want to thank people, institutions and companies who helped with financial and infrastructural support or scientific advice, and we are grateful to honor them on our <a href="https://2018.igem.org/Team:Marburg/Attributions">attributions page</a>. | Our project was only possible with a great <a href="https://2018.igem.org/Team:Marburg/Team">team</a> and we also want to thank people, institutions and companies who helped with financial and infrastructural support or scientific advice, and we are grateful to honor them on our <a href="https://2018.igem.org/Team:Marburg/Attributions">attributions page</a>. | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">Low amounts of DNA could be transformed reliably and we successfully demonstrated challenging clonings such as Gibson Assembly with 5 and Aquacloning with 3 fragments as well as 8 part golden-gate reactions</td> | + | <td class="scrollFade slideInRight" fadeTo="transformIn">Low amounts of DNA could be transformed reliably and we successfully demonstrated challenging clonings such as Gibson Assembly with 5 and Aquacloning with 3 fragments as well as 8 part golden-gate reactions.</td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
</tr> | </tr> | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">We designed and constructed the Marburg Collection, a highly flexible golden-gate based toolbox consisting of 123 parts | + | <td class="scrollFade slideInRight" fadeTo="transformIn">We designed and constructed the Marburg Collection, a highly flexible golden-gate based toolbox consisting of 123 parts. |
</td> | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">Dozens of test constructs were built and tested to obtain characterization data for all part categories in our toolbox | + | <td class="scrollFade slideInRight" fadeTo="transformIn">Dozens of test constructs were built and tested to obtain characterization data for all part categories in our toolbox. |
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<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">This was only possible with our novel experimental and data analysis workflow using platereader measurements | + | <td class="scrollFade slideInRight" fadeTo="transformIn">This was only possible with our novel experimental and data analysis workflow using platereader measurements. |
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">Subsequently, we were able to use Flp/FRT recombinase system for marker recycling thus allowing additional rounds of genomic modifications</td> | + | <td class="scrollFade slideInRight" fadeTo="transformIn">Subsequently, we were able to use Flp/FRT recombinase system for marker recycling thus allowing additional rounds of genomic modifications.</td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">Chromosomal locations suitable for genomic integration were identified and characterized to detect possible fitness effects </td> | + | <td class="scrollFade slideInRight" fadeTo="transformIn">Chromosomal locations suitable for genomic integration were identified and characterized to detect possible fitness effects. </td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">We accelerated metabolic engineering by developing a workflow for rapid pathway assembly and pathway optimization in <i>V. natriegens</i></td> | + | <td class="scrollFade slideInRight" fadeTo="transformIn">We accelerated metabolic engineering by developing a workflow for rapid pathway assembly and pathway optimization in <i>V. natriegens</i>.</td> |
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">Model-predicted parts were used to construct a pathway for maximal 3-hydroxypropionate production in <i>V. natriegens</i> and we demonstrated its functionality</td> | + | <td class="scrollFade slideInRight" fadeTo="transformIn">Model-predicted parts were used to construct a pathway for maximal 3-hydroxypropionate production in <i>V. natriegens</i> and we demonstrated its functionality.</td> |
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
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| − | <td class="scrollFade slideInRight" fadeTo="transformIn">Via mass spectrometry, we detected our pathway product 3-hydroxypropionic acid in <i>V. natriegens</i> | + | <td class="scrollFade slideInRight" fadeTo="transformIn">Via mass spectrometry, we detected our pathway product 3-hydroxypropionic acid in <i>V. natriegens</i>. |
</td> | </td> | ||
<td><div class="checkmark"></div></td> | <td><div class="checkmark"></div></td> | ||
Revision as of 02:12, 18 October 2018
Strain Engineering
Marburg Collection
Metabolic Engineering
Interlab
Accessible Science
