Difference between revisions of "Team:IIT Delhi/Demonstrate"
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<body> | <body> | ||
| − | + | <div id="globalWrapperdivi" style="background-color: white;margin-top: -6.5%;z-index: 1;"> | |
| − | <div class= "NameClass" style="background-color:white;"> | + | <div class="NameClass" style="background-color:white;"> |
<a style="height: 150px; width: 150px;margin-left: 34%;margin-top: 0px; padding-top: 35px;" href="https://2018.igem.org/Team:IIT_Delhi"> | <a style="height: 150px; width: 150px;margin-left: 34%;margin-top: 0px; padding-top: 35px;" href="https://2018.igem.org/Team:IIT_Delhi"> | ||
<img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px;display:inline; margin-top: 40px;"> </a> | <img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px;display:inline; margin-top: 40px;"> </a> | ||
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</div> | </div> | ||
| − | <div class="RespIcon" | + | <div class="RespIcon" style="background-color: white;"> |
<a href="https://2018.igem.org/Team:IIT_Delhi" style="height: 150px; width: 150px; margin-left: 5%;margin-top: 0px; padding-top: 14px;"> | <a href="https://2018.igem.org/Team:IIT_Delhi" style="height: 150px; width: 150px; margin-left: 5%;margin-top: 0px; padding-top: 14px;"> | ||
<img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px; display:inline; padding-top: 15px;"> </a> <a href="javascript:void(0);" style="Background-color: white;" class="icon" onclick="openNav()"> <i class="fa fa-bars" style="padding-top: 100px"></i> </a> | <img src="https://static.igem.org/mediawiki/2018/d/de/T--IIT_Delhi--whitelogo.png" style="height: 150px; width: 150px; display:inline; padding-top: 15px;"> </a> <a href="javascript:void(0);" style="Background-color: white;" class="icon" onclick="openNav()"> <i class="fa fa-bars" style="padding-top: 100px"></i> </a> | ||
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| − | <div class = "navaman"> | + | <div class="navaman"> |
<div class="dropdownsakks"> | <div class="dropdownsakks"> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="javascript:void(0)"> Notebook </a> | <a href="javascript:void(0)"> Notebook </a> | ||
</div> | </div> | ||
| − | <div class="dropdownsakks-content" | + | <div class="dropdownsakks-content" style="margin-top: 66px;"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Notebook">Lab Records</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Notebook">Lab Records</a> | ||
<a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi">Medal Criteria</a> | <a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi">Medal Criteria</a> | ||
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<div class="dropdownsakks"> | <div class="dropdownsakks"> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="javascript:void(0)"> Team </a> | <a href="javascript:void(0)"> Team </a> | ||
</div> | </div> | ||
| − | <div class="dropdownsakks-content" | + | <div class="dropdownsakks-content" style="margin-top: 66px;"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Team">Members</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Team">Members</a> | ||
<a href="https://2018.igem.org/Team:IIT_Delhi/Attributions">Attributions</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Attributions">Attributions</a> | ||
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</div> | </div> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations"> Collaboration</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations"> Collaboration</a> | ||
</div> | </div> | ||
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<div class="dropdownsakks"> | <div class="dropdownsakks"> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="javascript:void(0)"> Human Practices </a> | <a href="javascript:void(0)"> Human Practices </a> | ||
</div> | </div> | ||
| − | <div class="dropdownsakks-content" | + | <div class="dropdownsakks-content" style="margin-top: 66px;"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices">Human Practices</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices">Human Practices</a> | ||
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</div> | </div> | ||
</div> | </div> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Model">Modelling</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Model">Modelling</a> | ||
</div> | </div> | ||
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| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="javascript:void(0)"> Parts </a> | <a href="javascript:void(0)"> Parts </a> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="dropdownsakks"> | <div class="dropdownsakks"> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
| − | <a href="javascript:void(0)" >Results</a> | + | <a href="javascript:void(0)">Results</a> |
</div> | </div> | ||
| − | <div class="dropdownsakks-content" | + | <div class="dropdownsakks-content" style="margin-top: 66px;"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate">Demonstrate</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate">Demonstrate</a> | ||
<a href="https://2018.igem.org/Team:IIT_Delhi/InterLab">Interlab</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab">Interlab</a> | ||
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<div class="dropdownsakks"> | <div class="dropdownsakks"> | ||
| − | <div class= "borderYtoX"> | + | <div class="borderYtoX"> |
<a href="javascript:void(0)"> Project </a> | <a href="javascript:void(0)"> Project </a> | ||
</div> | </div> | ||
| − | <div class="dropdownsakks-content" | + | <div class="dropdownsakks-content" style="margin-top: 66px;"> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Description">Overview</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Description">Overview</a> | ||
<a href="https://2018.igem.org/Team:IIT_Delhi/Design">Design</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Design">Design</a> | ||
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</div> | </div> | ||
| − | <div class= "overlayved" id="myNav"> | + | <div class="overlayved" id="myNav"> |
| − | <!-- <a href="javascript:void(0)" class="closebtn" onclick="closeNav()">& | + | <!-- <a href="javascript:void(0)" class="closebtn" onclick="closeNav()">×</a> --> |
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<hr> | <hr> | ||
| − | <button class="overlayved-btn2" style="padding-bottom: 15px;padding-top: 15px;align-self: center;font-size: 30px;font-family: raleway;border-color: white;border-style: none; border-image: none;background-color: white;border-width: 0px;cursor: pointer; color: #818181;" | + | <button class="overlayved-btn2" style="padding-bottom: 15px;padding-top: 15px;align-self: center;font-size: 30px;font-family: raleway;border-color: white;border-style: none; border-image: none;background-color: white;border-width: 0px;cursor: pointer; color: #818181;" onclick="closeNav()">Close Navigation</button> |
<hr> | <hr> | ||
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<div style="height: 28px;"> </div> | <div style="height: 28px;"> </div> | ||
| − | <button class="overlayved-btn" style="padding-bottom: 15px;" | + | <button class="overlayved-btn" style="padding-bottom: 15px;" onclick="myFunction1()">Project |
<i class="fa fa-caret-right"></i> | <i class="fa fa-caret-right"></i> | ||
</button> | </button> | ||
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| − | <div class="dropdownsakks-container" id="mydropdownsakks1" style= "font-style: italic; "> | + | <div class="dropdownsakks-container" id="mydropdownsakks1" style="font-style: italic; "> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Description" style="font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;">Overview</a> | <a href="https://2018.igem.org/Team:IIT_Delhi/Description" style="font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;">Overview</a> | ||
<a href="https://2018.igem.org/Team:IIT_Delhi/Design" style="font-size: 15px;padding: 10px; padding-top: 5px; | <a href="https://2018.igem.org/Team:IIT_Delhi/Design" style="font-size: 15px;padding: 10px; padding-top: 5px; | ||
| − | padding-bottom: 0px;" >Design</a> | + | padding-bottom: 0px;">Design</a> |
</div> | </div> | ||
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</button> | </button> | ||
| − | <div class="dropdownsakks-container" id="mydropdownsakks2" style= "font-style: italic;"> | + | <div class="dropdownsakks-container" id="mydropdownsakks2" style="font-style: italic;"> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Basic Parts</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Basic_Part" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;">Basic Parts</a> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Composite_Part" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Composite Parts</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Composite_Part" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;">Composite Parts</a> |
</div> | </div> | ||
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</button> | </button> | ||
| − | <div class="dropdownsakks-container" id="mydropdownsakks6" style= "font-style: italic;"> | + | <div class="dropdownsakks-container" id="mydropdownsakks6" style="font-style: italic;"> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;" >Demonstrate</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Demonstrate" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 3px;">Demonstrate</a> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;" >Interlab</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/InterLab" style="font-size: 15px;font-size: 15px; padding: 10px; padding-bottom: 5px; padding-top: 5px;">Interlab</a> |
</div> | </div> | ||
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Model" style="padding-bottom: 15px;padding-top: 15px;" >Modelling</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Model" style="padding-bottom: 15px;padding-top: 15px;">Modelling</a> |
| − | <button class="overlayved-btn" onclick="myFunction3()" style="padding-bottom: 15px;" >Human Practices | + | <button class="overlayved-btn" onclick="myFunction3()" style="padding-bottom: 15px;">Human Practices |
<i class="fa fa-caret-right"></i> | <i class="fa fa-caret-right"></i> | ||
</button> | </button> | ||
| − | <div class="dropdownsakks-container" id="mydropdownsakks3" style= "font-style: italic;" | + | <div class="dropdownsakks-container" id="mydropdownsakks3" style="font-style: italic;"> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; " >Human Practices</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Human_Practices" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; ">Human Practices</a> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Safety" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Safety</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Safety" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;">Safety</a> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement" style="font-size: 15px;padding: 10px; padding-top: 5px; | <a href="https://2018.igem.org/Team:IIT_Delhi/Public_Engagement" style="font-size: 15px;padding: 10px; padding-top: 5px; | ||
| − | padding-bottom: 0px;" >Public Engagement</a> | + | padding-bottom: 0px;">Public Engagement</a> |
</div> | </div> | ||
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations" style="padding-bottom: 15px;padding-top: 15px;" >Collaboration</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Collaborations" style="padding-bottom: 15px;padding-top: 15px;">Collaboration</a> |
| − | <button class="overlayved-btn" onclick="myFunction4()" style="padding-bottom: 15px;padding-top: 15px;" | + | <button class="overlayved-btn" onclick="myFunction4()" style="padding-bottom: 15px;padding-top: 15px;">Team |
<i class="fa fa-caret-right"></i> | <i class="fa fa-caret-right"></i> | ||
</button> | </button> | ||
| − | <div class="dropdownsakks-container" id="mydropdownsakks4" style= "font-style: italic;"> | + | <div class="dropdownsakks-container" id="mydropdownsakks4" style="font-style: italic;"> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Team" style="font-size: 15px; font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;padding-bottom: 5px;" >Members</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Team" style="font-size: 15px; font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px;padding-bottom: 5px;">Members</a> |
<a href="https://2018.igem.org/Team:IIT_Delhi/Attributions" style="font-size: 15px;padding: 10px; padding-top: 5px; | <a href="https://2018.igem.org/Team:IIT_Delhi/Attributions" style="font-size: 15px;padding: 10px; padding-top: 5px; | ||
| − | padding-bottom: 5px;" >Attributions</a> | + | padding-bottom: 5px;">Attributions</a> |
</div> | </div> | ||
| − | <button class="overlayved-btn" onclick="myFunction5()" style="padding-bottom: 15px;" >Notebook | + | <button class="overlayved-btn" onclick="myFunction5()" style="padding-bottom: 15px;">Notebook |
<i class="fa fa-caret-right"></i> | <i class="fa fa-caret-right"></i> | ||
</button> | </button> | ||
| − | <div class="dropdownsakks-container" id="mydropdownsakks5" style= "font-style: italic;"> | + | <div class="dropdownsakks-container" id="mydropdownsakks5" style="font-style: italic;"> |
| − | <a href="https://2018.igem.org/Team:IIT_Delhi/Notebook" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; " >Lab Records</a> | + | <a href="https://2018.igem.org/Team:IIT_Delhi/Notebook" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 0px; ">Lab Records</a> |
| − | <a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;" >Medal Criteria</a> | + | <a href="https://igem.org/2018_Judging_Form?team=IIT_Delhi" style="font-size: 15px;font-size: 15px; padding-bottom: 5px; padding: 10px; padding-top: 5px;">Medal Criteria</a> |
</div> | </div> | ||
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| − | <div style="color: | + | <div style="color: black;background-color:white;text-align:center;padding:50px 80px;text-align: center;width: 75%;margin: auto;"> |
| − | <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;"> | + | <p style="font-size:20px;font-family: 'Lato', sans-serif;font-weight:400;text-align: center;"> |
| − | We extensively performed 3A assembly to develop these <a href" | + | We extensively performed 3A assembly to develop these <a href"http:="" 2018.igem.org="" team:iit_delhi="" composite_part"=""> composite parts.</a><br> |
These parts also include our second nodes and our third nodes for the incoherent feedforward loop design. Keeping in mind the expression of gene and compatibility of plasmids, the first node was placed in an expression vector containing ColE1 origin with chloramphenicol resistance, the second node in the expression vector containing p15A origin with Kanamycin resistance and the third node in the expression vector containing pSC101 origin with Ampicillin resistance. These compatible plasmids were cotransformed in E. coli DH5alpha Z1 strain to realize our project. <br> | These parts also include our second nodes and our third nodes for the incoherent feedforward loop design. Keeping in mind the expression of gene and compatibility of plasmids, the first node was placed in an expression vector containing ColE1 origin with chloramphenicol resistance, the second node in the expression vector containing p15A origin with Kanamycin resistance and the third node in the expression vector containing pSC101 origin with Ampicillin resistance. These compatible plasmids were cotransformed in E. coli DH5alpha Z1 strain to realize our project. <br> | ||
<br> | <br> | ||
Below we have provided the gel electrophoresis results for our final construction of the composite parts via 3A assembly of the various components step-by-step. The observed size of the DNA in the gel matches with the expected size of DNA, as proposed in the design.<br> | Below we have provided the gel electrophoresis results for our final construction of the composite parts via 3A assembly of the various components step-by-step. The observed size of the DNA in the gel matches with the expected size of DNA, as proposed in the design.<br> | ||
<br> | <br> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/b/ba/T--IIT_Delhi--Linear.jpg" style="border:3px solid #000000" width="580px" height="690px"> | + | <img src="https://static.igem.org/mediawiki/2018/b/ba/T--IIT_Delhi--Linear.jpg" style="border:3px solid #000000;height: 500px;width: 400px;" width="580px" height="690px"> |
| − | <img src="https://static.igem.org/mediawiki/2018/4/49/T--IIT_Delhi--DoubleDigest.jpg" style=" | + | <img src="https://static.igem.org/mediawiki/2018/4/49/T--IIT_Delhi--DoubleDigest.jpg" style="height: 500px;width: 400px;" width="520px"><br> |
</p> | </p> | ||
</div> | </div> | ||
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<div style="background-color: white"> | <div style="background-color: white"> | ||
| − | <div | + | <div style="background-color: #555;color:black;font-size: 35px;text-align: center;font-family: sans-serif;"><h4 style="color: white">Contact us</h4></div> |
<div style="text-align: center;margin-bottom: -136px;"><h4 style="color:black;font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br> | <div style="text-align: center;margin-bottom: -136px;"><h4 style="color:black;font-size: 50px;"><u class="fx" style="cursor: pointer;text-align: center">Address</u></h4><p style="text-align: center;margin-bottom: 16px">Undergraduate Laboratory<br> | ||
Department of Biotechnology and Biochemical Engineering, IIT Delhi</p> | Department of Biotechnology and Biochemical Engineering, IIT Delhi</p> | ||
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<div style="margin-left: 4.5%;margin-top: -120px;display: block;height: auto;max-height: inherit;width: auto;max-width: inherit;"><a href="iitd.igem@gmail.com" style="color:black;"><i class="fa fa-envelope fxm" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></a></div> | <div style="margin-left: 4.5%;margin-top: -120px;display: block;height: auto;max-height: inherit;width: auto;max-width: inherit;"><a href="iitd.igem@gmail.com" style="color:black;"><i class="fa fa-envelope fxm" style="font-size:90px;transition: 0.5s;transition-duration: 0.3s;transition-property: transform;margin-bottom: 30px;"></i></a></div> | ||
</div> | </div> | ||
| − | </div> | + | </div> <script> |
| − | + | ||
function openNav() { | function openNav() { | ||
Latest revision as of 02:27, 18 October 2018
We extensively performed 3A assembly to develop these composite parts.
These parts also include our second nodes and our third nodes for the incoherent feedforward loop design. Keeping in mind the expression of gene and compatibility of plasmids, the first node was placed in an expression vector containing ColE1 origin with chloramphenicol resistance, the second node in the expression vector containing p15A origin with Kanamycin resistance and the third node in the expression vector containing pSC101 origin with Ampicillin resistance. These compatible plasmids were cotransformed in E. coli DH5alpha Z1 strain to realize our project.
Below we have provided the gel electrophoresis results for our final construction of the composite parts via 3A assembly of the various components step-by-step. The observed size of the DNA in the gel matches with the expected size of DNA, as proposed in the design.
