Difference between revisions of "Team:Ecuador/Parts"

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{{Ecuador}}
 
{{Ecuador}}
 
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<title>C-lastin, Modeling</title>
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<div class="ec--first--section">
 
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<div class="ec--main--title">
 
<div class="ec--main--title">
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<a name="MOD"  style="text-decoration: none;"><h1>Collaborations</h1></a>
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<a name="MOD"  style="text-decoration: none;"><h1>PARTS</h1></a>
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<div class="ec--modeling--items">
 
<div class="ec--modeling--items">
 
<h3>Index</h3>
 
<h3>Index</h3>
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<ul>
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<ul class="circle">
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<li><a href="#l1">Estonia</a></li>
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<a href="#INT" style="text-decoration: none;color: #000000"><li>Introduction</li></a>
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<li><a href="#l2">Spain</a></li>
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<a href="#BAC" style="text-decoration: none;color: #000000"><li>Bone and cartilage regeneration</li></a>
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<li><a href="#l3">Mexico</a></li>
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<a href="#ELA" style="text-decoration: none;color: #000000"><li>Elasticity</li></a>
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<li><a href="#l4">MEETUP Latin America</a></li>
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<a href="#CELL" style="text-decoration: none;color: #000000"><li>Cellulose production </li></a>
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<li><a href="#l5">Chile</a></li>
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<li><a href="#l6">Surveys, Supporting development in iGEM.</a></li>
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<a name="INT">
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<div class="ec--h2">
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INTRODUCTION
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</div>
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<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
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</a>
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lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
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                <div class="ec--p">
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mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
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The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed).
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</div>
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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<a name="BAC">
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lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
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<div class="ec--h2">
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mso-ansi-language:EN-US'>Collaborations have been one of the greatest parts of
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Fusion protein for bone and cartilage regeneration
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iGEM, it was amazing to meet lovers of the science from all over the world,
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</div>
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share whit them and make contacts. It was wonderful to hear all the ideas of
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</a>
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their projects and share our idea too. <o:p></o:p></span></p>
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<div class="ec--h3">Coding parts</div>
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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<div class="ec--BMP--container">
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lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
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<a href="http://parts.igem.org/Part:BBa_K2804000"><div class="ec--h4">BBa_K2804000   
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mso-ansi-language:EN-US'>As newbies, we asked many questions and all the teams
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were always willing to answer, thank you very much. We shared whit teams from
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      </div></a>
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Mexico, Estonia, <span class=SpellE>España</span>, Chile and we participated in
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<strong>CBD cipA with a C-terminal linker</strong>
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the <span class=SpellE>MeetUp</span> <span class=SpellE>Latinametica</span>.
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<div class="ec--BMP--text">
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Below are the different iGEM teams that have collaborated with us.<o:p></o:p></span></p>
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    <div class="ec--p">This part was design to try to improve the imperial college (2014) CBD cipA part  BBa_K1321014, because the CBD N-terminal is where the protein binds to the cellulose and the linker could interfer with this interaction.</div>
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  </div>
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<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
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  </div>
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<div class="ec--BMP--container">
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flat;mso-style-textoutline-outlinestyle-join:round;mso-style-textoutline-outlinestyle-pctmiterlimit:
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<a href="http://parts.igem.org/Part:BBa_K2804006"><div class="ec--h4">
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0%;mso-style-textoutline-outlinestyle-dash:solid;mso-style-textoutline-outlinestyle-align:
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BBa_K2804006 
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center;mso-style-textoutline-outlinestyle-compound:simple;mso-effects-shadow-color:
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</a>
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mso-effects-shadow-align:center;mso-effects-shadow-pctsx:100.0%;mso-effects-shadow-pctsy:
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<strong>Superfolder GFP with six-histidine tail</strong>
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<div class="ec--BMP--text">
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EN-US'><a name="l1" style="text-decoration: none"></a>ESTONIA </span><u><span lang=EN-US style='font-family:"Arial",sans-serif;
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    <div class="ec--p">The adition of an histidine tail to part BBa_K1365020 was done because it allows the characterization of fusion proteins binding sites through atomic force microscopy, thanks to its affinity to the equipment nickel tip.</div>
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mso-ansi-language:EN-US'><o:p></o:p></span></u></p>
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  </div>
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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  </div>
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lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-ansi-language:EN-US'>The <a href="https://2018.igem.org/Team:Tartu_TUIT">iGEM team</a> from Tartu Estonia, is working in the
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<div class="ec--BMP--container">
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production of <span class=SpellE>mycosporine</span>-like amino acids, UV
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<a href="http://parts.igem.org/Part:BBa_K2804002"><div class="ec--h4">BBa_K2804002         
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radiation protection compounds, in yeast using the genes from two different
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      </div></a>
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cyanobacterial species. This compounds work not only as sunscreen but also are
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<strong>Bone morphogenetic protein 2 (BMP2)</strong>
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able to protect skin from photoaging, have the anti-inflammatory effect and
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<div class="ec--BMP--text">
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antioxidant activity and even could be considered as anti-cancer agents. <o:p></o:p></span></p>
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    <div class="ec--p">
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+
    Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.<sup>1</sup>  
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
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    </div>
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mso-ansi-language:EN-US'>As Ecuador is located on the equatorial line or
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  </div>
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parallel 0 °, is subjected to extremely high UV radiation indices, especially
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in the Andean area. In our country, there are constant campaigns to prevent
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<div class="ec--h3">Composite parts</div>
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skin cancer and protection against intense radiation.<o:p></o:p></span></p>
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−
 
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<div class="ec--BMP--container">
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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<a href="http://parts.igem.org/Part:BBa_K2804001"><div class="ec--h4">BBa_K2804001 
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lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
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      </div></a>
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mso-ansi-language:EN-US'>In the meetings with the iGEM team from Tartu we
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<strong>CBD cipA fused to BMP2 under the control of LacI promoter</strong>
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talked about the importance of your project and how it would benefit tropical
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<div class="ec--BMP--text">
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countries like ours. We also comment on experiences on how the sunscreens
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    <div class="ec--p">Therapeutic protein coupled to the CBD whose expression is regulated by the presence of IPTG in the medium.</div>
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contain chemical compounds which are extremely toxic to the oceans and the
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  </div>
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environment.<o:p></o:p></span></p>
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  </div>
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<div id="slider_Estonia">
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<div class="ec--BMP--container">
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<img src="https://static.igem.org/mediawiki/2018/6/6b/T--Ecuador--Estonia.png" data-src-2x="https://static.igem.org/mediawiki/2018/6/6b/T--Ecuador--Estonia.png"  />
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<a href="http://parts.igem.org/Part:BBa_K2804004"><div class="ec--h4">BBa_K2804004
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<img data-src="https://static.igem.org/mediawiki/2018/7/7f/T--Ecuador--estonia2.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/7/7f/T--Ecuador--estonia2.jpg" src=""  />
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      </div></a>
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<img data-src="https://static.igem.org/mediawiki/2018/6/6b/T--Ecuador--Estonia.png" data-src-2x="https://static.igem.org/mediawiki/2018/6/6b/T--Ecuador--Estonia.png" src=""  />
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CBD cipA fused to sfGFP
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<img data-src="https://static.igem.org/mediawiki/2018/7/7f/T--Ecuador--estonia2.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/7/7f/T--Ecuador--estonia2.jpg" src=""  />
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<div class="ec--BMP--text">
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<a href="http://parts.igem.org/Part:BBa_K2804005"><div class="ec--h4">BBa_K2804005
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      </div></a>
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100.0%;mso-effects-shadow-anglekx:0;mso-effects-shadow-angleky:0'><a name="l2" style="text-decoration: none"></a>SPAIN<o:p></o:p></span></p>
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<p class=MsoNormal style='line-height:150%'><span class=SpellE><span
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lang=EN-US style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-fareast-font-family:"Times New Roman";color:#353535;mso-ansi-language:EN-US;
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mso-fareast-language:ES-EC'>SynBio</span></span><span lang=EN-US
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style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-fareast-language:ES-EC'> has never been easier, <a href="https://2018.igem.org/Team:Valencia_UPV">UPV Valencia</a> is working in
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&quot;<span class=SpellE>Printeria</span>&quot;, a fully equipped
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bioengineering device, literally a mini lab that opens the door to a world of
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affordable applications for the general public. <span class=SpellE>Printeria</span>
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is able to automate the process of printing genetic circuits in bacteria and is
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as easy to operate as a common printer. It uses a system of digital
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microfluidics that creates small droplets that can be mixed and moved through
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predefined electrode trajectories. This box lab is combined with Golden Gate
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technology, sensors and low-cost electronics and an easy-to-use software
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application.
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<o:p></o:p>
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</span></p>
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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lang=EN-US style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-fareast-font-family:"Times New Roman";color:#353535;mso-ansi-language:EN-US;
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mso-fareast-language:ES-EC'>Just like that the user is able to assemble parts
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of DNA domesticated in a one-step reaction and can control all biotechnological
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steps, from the assembly of parts and transformation to cell culture, with high
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precision.<o:p></o:p></span></p>
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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lang=EN-US style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-fareast-font-family:"Times New Roman";color:#353535;mso-ansi-language:EN-US;
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mso-fareast-language:ES-EC'>The initial idea was to try the production of the
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fusion proteins and bacterial cellulose in <span class=SpellE>Printeria</span>.
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Can you imagen that hospital would produce his own bandages when they need it? But
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both teams have problems with the size of the parts, so we change plans and
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decided to evaluate the opinion of researchers, <span class=SpellE>medicians</span>
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and artist.<o:p></o:p></span></p>
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    <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
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    style='mso-ansi-language:EN-US'>Survey: Spain <o:p></o:p></span></p>
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    </div>
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    <![if !mso]></td>
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    <div>
+
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    <p class=MsoNormal align=center style='text-align:center'><span lang=EN-US
+
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    style='mso-ansi-language:EN-US'>Survey: Ecuador<o:p></o:p></span></p>
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    </div>
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    <![if !mso]></td>
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  </tr>
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</v:rect><![endif]--><![if !vml]><![endif]><span
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class=SpellE><span lang=ES-EC style='font-size:10.0pt;line-height:150%;
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font-family:"Arial",sans-serif;mso-fareast-font-family:"Times New Roman";
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color:#353535;mso-fareast-language:ES-EC'>Spain</span></span><span lang=ES-EC
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style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-fareast-font-family:"Times New Roman";color:#353535;mso-fareast-language:
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ES-EC'>: </span><span lang=ES-EC style='font-family:"Arial",sans-serif'><a
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href="https://youtu.be/ITVeAXSsO5k"><span style='font-size:10.0pt;line-height:
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150%;mso-fareast-font-family:"Times New Roman";mso-fareast-language:ES-EC'>https://youtu.be/ITVeAXSsO5k</span></a></span><span
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lang=ES-EC style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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mso-fareast-font-family:"Times New Roman";color:#353535;mso-fareast-language:
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ES-EC'><span style='mso-spacerun:yes'>          </span><span
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style='mso-spacerun:yes'> </span><span style='mso-spacerun:yes'>        </span><o:p></o:p>&nbsp;&nbsp; <button onclick="window.location.href='https://static.igem.org/mediawiki/2018/3/31/T--Ecuador--spain_survey.pdf'">Survey Spain</button>
+
−
</span>    </p>
+
−
 
+
−
<p class=MsoNormal style='text-align:justify;line-height:150%'><span
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−
lang=ES-EC style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
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−
mso-fareast-font-family:"Times New Roman";color:#353535;mso-fareast-language:
+
−
ES-EC'>Ecuador: </span><span lang=ES-EC style='font-family:"Arial",sans-serif'><a
+
−
href="https://youtu.be/LDC6Ud6j1JY"><span style='font-size:10.0pt;line-height:
+
−
150%;mso-fareast-font-family:"Times New Roman";mso-fareast-language:ES-EC'>https://youtu.be/LDC6Ud6j1JY</span></a></span><span
+
−
lang=ES-EC style='font-size:10.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-fareast-font-family:"Times New Roman";color:#353535;mso-fareast-language:
+
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ES-EC'> <o:p></o:p>  &nbsp;
+
−
<button onclick="myFunction('https://static.igem.org/mediawiki/2018/b/b2/T--Ecuador--Ecuador_survey.pdf')">Survey Ecuador</button></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
10.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-font-family:
+
−
"Times New Roman";color:#353535;mso-ansi-language:EN-US;mso-fareast-language:
+
−
ES-EC'>Also, the iGEM team Valencia UPV provided us with an E. coli strain
+
−
called BL21(DE3), thank you very much. <o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
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12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-language:
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ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="Imagen_x0020_1" o:spid="_x0000_i1036"
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type="#_x0000_t75" alt="La imagen puede contener: 11 personas, personas sonriendo, personas sentadas"
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style='width:283.2pt;height:162pt;visibility:visible;mso-wrap-style:square'>
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<v:imagedata src="Collaborations_archivos/image007.jpg" o:title=" 11 personas, personas sonriendo, personas sentadas"/>
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</v:shape><![endif]--><![if !vml]></span></p>
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<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
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12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-language:
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ES-EC;mso-no-proof:yes'><![endif]></span><span
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lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif'><o:p></o:p></span></p>
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<img src="https://static.igem.org/mediawiki/2018/f/f0/T--Ecuador--Valencia1.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/f/f0/T--Ecuador--Valencia1.jpg"  />
+
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<img data-src="https://static.igem.org/mediawiki/2018/3/31/T--Ecuador--Valencia2.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/3/31/T--Ecuador--Valencia2.jpg"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/f/f0/T--Ecuador--Valencia1.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/f/f0/T--Ecuador--Valencia1.jpg" src="" alt="Estonia"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/3/31/T--Ecuador--Valencia2.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/3/31/T--Ecuador--Valencia2.jpg"  />
+
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+
 
</div>
 
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28.0pt;mso-bidi-font-size:36.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
<a href="http://parts.igem.org/Part:BBa_K2804014"><div class="ec--h4">BBa_K2804014
−
mso-style-textoutline-type:none;mso-style-textoutline-outlinestyle-dpiwidth:
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      </div></a>
−
0pt;mso-style-textoutline-outlinestyle-linecap:flat;mso-style-textoutline-outlinestyle-join:
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−
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<div class="ec--BMP--text">
−
solid;mso-style-textoutline-outlinestyle-align:center;mso-style-textoutline-outlinestyle-compound:
+
  <div class="ec--p"></div>
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simple;mso-effects-shadow-color:#6E747A;mso-effects-shadow-alpha:43.0%;
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mso-effects-shadow-pctsx:100.0%;mso-effects-shadow-pctsy:100.0%;mso-effects-shadow-anglekx:
+
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0;mso-effects-shadow-angleky:0'><a name="l3" style="text-decoration: none"></a>MEXICO</span><span lang=ES-EC style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif'> <o:p></o:p></span></p>
+
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+
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<p class=MsoNormal style='line-height:150%'><span class=SpellE><b
+
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style='mso-bidi-font-weight:normal'><span lang=ES-EC style='font-size:12.0pt;
+
−
line-height:150%;font-family:"Arial",sans-serif'><a href="https://2018.igem.org/Team:TecCEM/Collaborations" style="color: black">TecCEM</a></span></b></span><b
+
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style='mso-bidi-font-weight:normal'><span lang=ES-EC style='font-size:12.0pt;
+
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line-height:150%;font-family:"Arial",sans-serif'><o:p></o:p></span></b></p>
+
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+
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<p class=MsoNormal style='line-height:150%'><span
+
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class=GramE><span lang=EN-US style='font-family:"Arial",sans-serif;mso-ansi-language:
+
−
EN-US'>Also, <span style='mso-spacerun:yes'> </span>we</span></span><span
+
−
lang=EN-US style='font-family:"Arial",sans-serif;mso-ansi-language:EN-US'> do videocalls
+
−
to share ideas and feedback about our projects.
+
−
<o:p></o:p>
+
−
</span><span lang=EN-US style='font-size:
+
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12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'><br>
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</span><span lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:
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"Arial",sans-serif;mso-fareast-language:ES-EC;mso-no-proof:yes'>
+
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<!--[if gte vml 1]><v:shape
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+
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<![if !vml]><![endif]>
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</span></p>
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<p class=MsoNormal style='line-height:150%'></p>
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<p class=MsoNormal style='text-align:justify;line-height:150%'>&nbsp;</p>
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+
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<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
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lang=EN-US style='mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="image45.jpg"
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style='width:387.6pt;height:242.4pt;visibility:visible;mso-wrap-style:square'>
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+
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</v:shape><![endif]--><![if !vml]><img border=0 width=517 height=323
+
−
src="https://static.igem.org/mediawiki/2018/7/73/T--Ecuador--N24.2.jpeg"
+
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alt="https://scontent.fgye7-1.fna.fbcdn.net/v/t1.15752-9/43293037_1626139370823675_8290006412856131584_n.jpg?_nc_cat=111&amp;oh=3475c2df7d953aaf3664b8cdcca7df9b&amp;oe=5C4D65F0"
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v:shapes="image45.jpg"><![endif]></span><span lang=EN-US style='font-size:12.0pt;
+
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line-height:150%;font-family:"Arial",sans-serif;mso-fareast-font-family:Arial'><o:p></o:p></span></p>
+
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+
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<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
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12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
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+
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<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
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28.0pt;mso-bidi-font-size:36.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
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mso-style-textoutline-type:none;mso-style-textoutline-outlinestyle-dpiwidth:
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0pt;mso-style-textoutline-outlinestyle-linecap:flat;mso-style-textoutline-outlinestyle-join:
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round;mso-style-textoutline-outlinestyle-pctmiterlimit:0%;mso-style-textoutline-outlinestyle-dash:
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solid;mso-style-textoutline-outlinestyle-align:center;mso-style-textoutline-outlinestyle-compound:
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simple;mso-effects-shadow-color:#6E747A;mso-effects-shadow-alpha:43.0%;
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mso-effects-shadow-dpiradius:3.0pt;mso-effects-shadow-dpidistance:2.0pt;
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+
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0;mso-effects-shadow-angleky:0;mso-ansi-language:EN-US'><a name="l4" style="text-decoration: none"></a>MEETUP LATIN AMERICA<o:p></o:p></span></p>
+
−
 
+
−
+
−
<p class=MsoNormal style='line-height:150%'><span
+
−
lang=EN-US style='font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>The
+
−
past July, we had the chance to attend online at the <a href="https://2018.igem.org/Teams/Meetups#Latin_American_Meetup">Latin American Meetup</a> hosted by team <span class=SpellE>TecCEM</span>. There we heard about the other
+
−
Latin American's projects, sharing experiences and learning from each
+
−
other.<span style='mso-spacerun:yes'>  </span>We got the opportunity to present
+
−
our project and received good questions from experts that had helped us to be
+
−
prepared for the Giant Jamboree and gives us experience. The eight teams that
+
−
participated in the meetup were: <span class=SpellE>TecCEM</span>,
+
−
Tec-Chihuahua, Tec-Monterrey, <span class=SpellE>TecMonterrey</span>-<span
+
−
class=GramE>GDL ,</span> <span class=SpellE>UChile_Biotec</span>, Ecuador,
+
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USP-EEL-Brazil, USP-Brazil.
+
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<o:p></o:p>
+
−
</span></p>
+
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+
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<p class=MsoNormal style='text-align:justify;line-height:150%'><span
+
−
lang=EN-US style='font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>The
+
−
feedback provided by the specialists was crucial for the proper development of
+
−
the projects, and beyond the scientific issues, we were able to get to know
+
−
each other, get along and create bonds of collaboration and support between the
+
−
teams.<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='text-align:justify;line-height:150%'><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-fareast-language:ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="Imagen_x0020_7"
+
−
o:spid="_x0000_i1034" type="#_x0000_t75" style='width:425.4pt;height:186pt;
+
−
visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="Collaborations_archivos/image011.png" o:title="" croptop="18782f"
+
−
  cropbottom="12428f" cropleft="17653f" cropright="3844f"/>
+
−
</v:shape><![endif]--><![if !vml]><![endif]></span><span
+
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'><o:p></o:p></span></p>
+
−
<div id="slider_Latin">
+
−
<img src="https://static.igem.org/mediawiki/2018/b/b5/T--Ecuador--meet1.png" data-src-2x="https://static.igem.org/mediawiki/2018/b/b5/T--Ecuador--meet1.png"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/8/8a/T--Ecuador--meet2.png" data-src-2x="https://static.igem.org/mediawiki/2018/8/8a/T--Ecuador--meet2.png"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/b/b5/T--Ecuador--meet1.png" data-src-2x="https://static.igem.org/mediawiki/2018/b/b5/T--Ecuador--meet1.png" src="" />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/8/8a/T--Ecuador--meet2.png" data-src-2x="https://static.igem.org/mediawiki/2018/8/8a/T--Ecuador--meet2.png"  />
+
−
+
 
</div>
 
</div>
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
+
  <div class="ec--BMP--container">
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
<a href="http://parts.igem.org/Part:BBa_K2804015"><div class="ec--h4">BBa_K2804015
−
mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
      </div></a>
−
 
+
CBD cipA fused to BMP2
−
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;text-align:
+
<div class="ec--BMP--text">
−
justify;line-height:150%;background:white'><span lang=EN-US style='font-size:
+
  <div class="ec--p"></div>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>Dancing
+
</div>
−
in the lab is something that we all have done some time! This collaboration is
+
</div>
−
a prove. We participated in the creation of a music video with the songs: Labs
+
<table border="0" cellspacing="0" cellpadding="0" width="703">
−
like you and Moves like iGEM. Collaboration organized by <span class=SpellE>TecCEM</span>,
+
  <tr>
−
was one of the most fun and we spent an incredible time recording the video. <o:p></o:p></span></p>
+
    <td width="175" nowrap valign="bottom"><p><strong>Name</strong></p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>Description</strong></p></td>
−
<p class=MsoNormal style='margin-bottom:0in;margin-bottom:.0001pt;line-height:
+
    <td width="80" nowrap valign="bottom"><p><strong>Type</strong></p></td>
−
150%;background:white'><span lang=EN-US style='font-size:12.0pt;line-height:
+
      </tr>
−
150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
  <tr>
−
<div class="ec--coll--video">
+
    <td width="175"><p>BBa_K2804000</p></td>
−
  <center>
+
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA with a C-terminal linker</strong></p></td>
−
<iframe width="560" height="315" src="https://www.youtube.com/embed/R7vdiGLLJZA" frameborder="0" allow="autoplay; encrypted-media" allowfullscreen></iframe>
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
</center>
+
      </tr>
−
 
+
  <tr>
 +
    <td width="175"><p>BBa_K2804001</p></td>
 +
    <td width="448" nowrap valign="bottom"><p>CBD cipA fused to BMP2 under the control of LacI    promoter</p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804002</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>Bone morphogenetic protein 2 (BMP2)</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804004</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to sfGFP</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804005</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to sfGFP under the control    of LacI promoter</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804006</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>Superfolder GFP with six-histidine tail</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804014</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to sfGFP</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804015</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to BMP2</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  </table>
 +
<p>&nbsp;</p>
 +
 +
<a name="ELA">
 +
<div class="ec--h2">
 +
Fusion protein to provide elasticity to the biomaterial
 +
</div>
 +
</a>
 +
<div class="ec--h3">Coding parts</div>
 +
 +
<div class="ec--BMP--container">
 +
  <a href="http://parts.igem.org/Part:BBa_K2804011"><div class="ec--h4">BBa_K2804011   
 +
      </div></a>
 +
ELP V-150
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 
</div>
 
</div>
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
<div class="ec--BMP--container">
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-font-family:
+
  <a href="http://parts.igem.org/Part:BBa_K2804012"><div class="ec--h4">BBa_K2804012 
−
"Times New Roman";color:#222222;mso-ansi-language:EN-US;mso-fareast-language:
+
      </div></a>
−
ES-EC'><o:p>&nbsp;</o:p></span></p>
+
ELP V-150
−
 
+
<div class="ec--BMP--text">
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
  <div class="ec--p"></div>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>Below
+
</div>
−
is a list of all the teams that participated:<o:p></o:p></span></p>
+
</div>
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
<div class="ec--h3">Composite part</div>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>1.
+
−
<span class=SpellE>iGEM</span> IISER <span class=SpellE>Bhopa</span><o:p></o:p></span></p>
+
  <div class="ec--BMP--container">
−
 
+
<a href="http://parts.igem.org/Part:BBa_K2804013"><div class="ec--h4">BBa_K2804013   
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
      </div></a>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>2.
+
CBD cipA fused to ELP_C5 under the control of LacI promoter
−
IGEM AFCM <o:p></o:p></span></p>
+
<div class="ec--BMP--text">
−
 
+
  <div class="ec--p"></div>
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
</div>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>3.
+
−
IGEM UNESP BRAZIL<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>4.
+
−
IGEM WASHINGTON<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>5.
+
−
IGEM <span class=SpellE>Tust</span> China <o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>6.
+
−
<span class=SpellE>iGEM</span> team Bielefeld-<span class=SpellE>CeBiTec</span>
+
−
<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>7.
+
−
IISc-Bangalore <span class=SpellE>iGEM</span> Team<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>8.
+
−
IGEM BRAZIL USP<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>9.
+
−
IGEM DUSSELDORF<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>10.
+
−
IGEM GRONINGEN<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>11.
+
−
IGEM DALHOUSIE<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>12.
+
−
IGEM LEIDEN<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>13.
+
−
Team UFLORIDA<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>14.
+
−
<span class=SpellE>Tarta</span> TUIT_IGEM<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>15.
+
−
Toulouse INSA UPS<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>16.
+
−
IGEM MICHIGAN STATE<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>17.
+
−
Team <span class=SpellE>UCHILE_Biotec</span><o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif'>18. ECUADOR<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif'>19. IGEM BRAZIL USP EEL<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif'>20. <span class=SpellE>Tec</span>-Guadalajara<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>21.
+
−
Tec-Chihuahua<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>22.
+
−
UCSC IGEM<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>23.
+
−
<span class=SpellE>TecCEM</span><o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>24.
+
−
<span class=SpellE>iTESLA-SoundBio</span> Team<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>25.
+
−
Tacoma <span class=SpellE>RainMakers</span> Team<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-font-family:
+
−
"Times New Roman";color:#616770;background:white;mso-ansi-language:EN-US;
+
−
mso-fareast-language:ES-EC'><o:p>&nbsp;</o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-font-family:
+
−
"Times New Roman";color:#616770;background:white;mso-ansi-language:EN-US;
+
−
mso-fareast-language:ES-EC'><o:p>&nbsp;</o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><b style='mso-bidi-font-weight:
+
−
normal'><span lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:
+
−
"Arial",sans-serif;mso-fareast-font-family:"Times New Roman";color:#616770;
+
−
background:white;mso-ansi-language:EN-US;mso-fareast-language:ES-EC'><a href="https://2018.igem.org/Team:Tec-Monterrey" style="color: black">Tec-Monterrey</a><o:p></o:p>
+
−
</span></b></p>
+
−
 
+
−
+
−
<p class=MsoNormal style='line-height:150%'><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-fareast-language:ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="Imagen_x0020_3"
+
−
o:spid="_x0000_i1032" type="#_x0000_t75" alt="La imagen puede contener: 20 personas, personas sonriendo, personas sentadas e interior"
+
−
style='width:283.2pt;height:212.4pt;visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="Collaborations_archivos/image015.jpg" o:title=" 20 personas, personas sonriendo, personas sentadas e interior"/>
+
−
</v:shape><![endif]--><![if !vml]></span><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-fareast-font-family:"Times New Roman";color:#616770;background:white;
+
−
mso-fareast-language:ES-EC;mso-no-proof:yes'><![endif]></span><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif'>
+
−
<o:p></o:p>
+
−
</span></p>
+
−
<div id="slider_Mexico">
+
−
<img src="https://static.igem.org/mediawiki/2018/c/c4/T--Ecuador--teccem1.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/c/c4/T--Ecuador--teccem1.jpg" title="Estonia" alt="Reunion" />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/2/23/T--Ecuador--teccem2.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/2/23/T--Ecuador--teccem2.jpg"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/c/c4/T--Ecuador--teccem1.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/c/c4/T--Ecuador--teccem1.jpg" src="" alt="Estonia"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/2/23/T--Ecuador--teccem2.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/2/23/T--Ecuador--teccem2.jpg"  />
+
−
+
−
</div>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>It
+
−
was a great experience to have had a video conference with the Tec-Monterrey
+
−
team who welcomed us to IGEM because they were the first team we contacted.<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
36.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-style-textoutline-type:
+
−
none;mso-style-textoutline-outlinestyle-dpiwidth:0pt;mso-style-textoutline-outlinestyle-linecap:
+
−
flat;mso-style-textoutline-outlinestyle-join:round;mso-style-textoutline-outlinestyle-pctmiterlimit:
+
−
0%;mso-style-textoutline-outlinestyle-dash:solid;mso-style-textoutline-outlinestyle-align:
+
−
center;mso-style-textoutline-outlinestyle-compound:simple;mso-effects-shadow-color:
+
−
#6E747A;mso-effects-shadow-alpha:43.0%;mso-effects-shadow-dpiradius:3.0pt;
+
−
mso-effects-shadow-dpidistance:2.0pt;mso-effects-shadow-angledirection:5400000;
+
−
mso-effects-shadow-align:center;mso-effects-shadow-pctsx:100.0%;mso-effects-shadow-pctsy:
+
−
100.0%;mso-effects-shadow-anglekx:0;mso-effects-shadow-angleky:0;mso-ansi-language:
+
−
EN-US'><a name="l5" style="text-decoration: none"></a>CHILE <o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>We
+
−
participated in the collaboration organized by the <a href="https://2018.igem.org/Team:UChile_Biotec">UChile_Biotec team </a>that consisted in the creation of the Latin America magazine. The most
+
−
delicious collaboration, we exposed some delicious typical dishes of our
+
−
country such as the <span class=SpellE>guaguas</span> de pan, arroz de <span
+
−
class=SpellE>leche</span> and <span class=SpellE>fritada</span>. We thank the <span
+
−
class=SpellE>UChile_Biotec</span> team for giving us the opportunity to be part
+
−
of <a href="https://2018.igem.org/Team:UChile_Biotec/LATAM ">LATAM</a>:
+
−
<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%;tab-stops:121.2pt'><span lang=EN-US
+
−
style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'>
+
−
<o:p>&nbsp;</o:p>
+
−
</span></p>
+
−
<div id="slider_Chile">
+
−
<img src="https://static.igem.org/mediawiki/2018/c/c4/T--Ecuador--teccem1.jpg" data-src-2x="https://static.igem.org/mediawiki/2018/c/c4/T--Ecuador--teccem1.jpg" title="Chile" alt="From Ecuador" />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/c/cd/T--Ecuador--ChileLatam1.png" data-src-2x="https://static.igem.org/mediawiki/2018/c/cd/T--Ecuador--ChileLatam1.png" src="" title="" />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/2/26/T--Ecuador--Chile2.png" data-src-2x="https://static.igem.org/mediawiki/2018/2/26/T--Ecuador--Chile2.png"  />
+
−
<img data-src="https://static.igem.org/mediawiki/2018/8/80/T--Ecuador--Chile3.png" data-src-2x="https://static.igem.org/mediawiki/2018/8/80/T--Ecuador--Chile3.png" src="" alt="Chile"  />
+
−
+
−
+
 
</div>
 
</div>
−
<p class=MsoNormal style='line-height:150%;tab-stops:121.2pt'>&nbsp;</p>
+
<table border="0" cellspacing="0" cellpadding="0" width="703">
−
 
+
  <tr>
−
<p class=MsoNormal style='line-height:150%;tab-stops:121.2pt'>&nbsp;</p>
+
    <td width="175" nowrap valign="bottom"><p><strong>Name</strong></p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>Description</strong></p></td>
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'><b
+
    <td width="80" nowrap valign="bottom"><p><strong>Type</strong></p></td>
−
style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:14.0pt;
+
      </tr>
−
mso-bidi-font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
  <tr>
−
mso-ansi-language:EN-US'><a name="l6" style="text-decoration: none"></a>Surveys, Supporting development in iGEM.<o:p></o:p></span></b></p>
+
    <td width="175"><p>BBa_K2804011</p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>ELP V-150</strong></p></td>
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>iGEM
+
      </tr>
−
is a competition that brings together lovers of technology from all over the
+
  <tr>
−
world, passionate about the development of science and a way to know points of
+
    <td width="175"><p>BBa_K2804012</p></td>
−
view and opinions of all of them, are the surveys. Through social networks such
+
    <td width="448" nowrap valign="bottom"><p><strong>ELP_C5</strong></p></td>
−
as Instagram, <span class=SpellE>Faceook</span> and <span class=SpellE>Youtube</span>
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
we have contacted several teams that have asked for our opinion and below are
+
      </tr>
−
some of the surveys conducted during IGEM 2018 by the Ecuador team:<o:p></o:p></span></p>
+
  <tr>
−
 
+
    <td width="175"><p>BBa_K2804013</p></td>
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to ELP_C5 under the control    of LacI promoter</strong></p></td>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'><br>
+
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
−
<b style='mso-bidi-font-weight:normal'>Team iGEM Thessaloniki</b><br>
+
      </tr>
−
</span><span lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:
+
  </table>
−
"Arial",sans-serif;mso-fareast-language:ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape
+
<p>&nbsp;</p>
−
id="Imagen_x0020_14" o:spid="_x0000_i1030" type="#_x0000_t75" style='width:226.8pt;
+
−
height:91.2pt;visibility:visible;mso-wrap-style:square'>
+
<a name="CELL">
−
<v:imagedata src="Collaborations_archivos/image019.png" o:title="43638992_252708545412712_57567259144486912_n"
+
<div class="ec--h2">
−
  croptop="14958f" cropbottom="17304f" cropleft="9233f" cropright="9719f"/>
+
Cellulose production module
−
</v:shape><![endif]--><![if !vml]><img border=0 width=302 height=122
+
</div>
−
src="https://static.igem.org/mediawiki/2018/3/3d/T--Ecuador--survey1.png" v:shapes="Imagen_x0020_14"><![endif]></span><span
+
</a>
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
<div class="ec--h3">Coding parts</div>
−
color:black;background:#F1F0F0;mso-ansi-language:EN-US'><o:p></o:p></span></p>
+
−
 
+
<div class="ec--BMP--container">
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
  <a href="http://parts.igem.org/Part:BBa_K2804008">
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>
+
  <div class="ec--h4">
−
<o:p>&nbsp;</o:p>
+
  BBa_K2804008    
−
</span></p>
+
      </div>
−
 
+
  </a>
−
<p class=MsoNormal style='line-height:150%'><b style='mso-bidi-font-weight:
+
CmcAx: Endo-beta-1,4-glucanase ezyme
−
normal'><span lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:
+
<div class="ec--BMP--text">
−
"Arial",sans-serif;mso-ansi-language:EN-US'>Team Bielefeld-<span class=SpellE>CeBiTec</span>,
+
  <div class="ec--p"></div>
−
Germany. <o:p></o:p></span></b></p>
+
</div>
−
 
+
</div>
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
+
<div class="ec--BMP--container">
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;mso-fareast-language:
+
  <a href="http://parts.igem.org/Part:BBa_K2804009"><div class="ec--h4">BBa_K2804009 
−
ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="Imagen_x0020_15"
+
      </div></a>
−
o:spid="_x0000_i1029" type="#_x0000_t75" style='width:226.8pt;height:94.2pt;
+
CcpAx: Cellulose-complementing protein
−
visibility:visible;mso-wrap-style:square'>
+
<div class="ec--BMP--text">
−
<v:imagedata src="Collaborations_archivos/image021.png" o:title="42713155_2210879069195152_7441282302065246208_n"
+
  <div class="ec--p"></div>
−
  croptop="9178f" cropbottom="27434f" cropleft="13006f" cropright="13224f"/>
+
</div>
−
</v:shape><![endif]--><![if !vml]><img border=0 width=302 height=126
+
</div>
−
src="https://static.igem.org/mediawiki/2018/f/f2/T--Ecuador--survey2.png" v:shapes="Imagen_x0020_15"><![endif]></span><span
+
  </div>
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif'><o:p></o:p></span></p>
+
−
 
+
<div class="ec--h3">Composite part</div>
−
<p class=MsoNormal style='line-height:150%'>&nbsp;</p>
+
−
 
+
<div class="ec--BMP--container">
−
<p class=MsoNormal style='line-height:150%'><b style='mso-bidi-font-weight:
+
  <a href="http://parts.igem.org/Part:BBa_K2804010"><div class="ec--h4">BBa_K2804010 
−
normal'><span lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:
+
      </div></a>
−
"Arial",sans-serif;mso-ansi-language:EN-US'>Team Lund, Sweden.</span></b><span
+
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
<div class="ec--BMP--text">
−
mso-ansi-language:EN-US'><br>
+
  <div class="ec--p"></div>
−
</span><span lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:
+
</div>
−
"Arial",sans-serif;mso-fareast-language:ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape
+
</div>
−
id="Imagen_x0020_16" o:spid="_x0000_i1028" type="#_x0000_t75" style='width:226.8pt;
+
<table border="1" cellspacing="0" cellpadding="0" width="703">
−
height:102.6pt;visibility:visible;mso-wrap-style:square'>
+
  <tr>
−
<v:imagedata src="Collaborations_archivos/image023.png" o:title="43712999_306113363318407_2044872034575450112_n"
+
    <td width="175" nowrap valign="bottom"><p><strong>Name</strong></p></td>
−
   croptop="11190f" cropbottom="24796f" cropleft="15910f" cropright="15956f"/>
+
    <td width="448" nowrap valign="bottom"><p><strong>Description</strong></p></td>
−
</v:shape><![endif]--><![if !vml]><img border=0 width=302 height=137
+
    <td width="80" nowrap valign="bottom"><p><strong>Type</strong></p></td>
−
src="https://static.igem.org/mediawiki/2018/2/22/T--Ecuador--survey3.png" v:shapes="Imagen_x0020_16"><![endif]></span><span
+
      </tr>
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
  <tr>
−
mso-ansi-language:EN-US'><o:p></o:p></span></p>
+
    <td width="175"><p>BBa_K2804008</p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>CmcAx: Endo-beta-1,4-glucanase ezyme</strong></p></td>
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif'><a
+
      </tr>
−
href="https://static.igem.org/mediawiki/2018/2/22/T--Ecuador--survey3.png"></a></span><span
+
  <tr>
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
    <td width="175"><p>BBa_K2804009</p></td>
−
color:black;background:#F1F0F0;mso-ansi-language:EN-US'><o:p></o:p></span></p>
+
    <td width="448" nowrap valign="bottom"><p><strong>CcpAx: Cellulose-complementing protein</strong></p></td>
−
 
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
<p class=MsoNormal style='line-height:150%'><span lang=EN-US style='font-size:
+
      </tr>
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif;color:black;background:
+
  <tr>
−
#F1F0F0;mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
    <td width="175"><p>BBa_K2804010</p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>Operon member of bacterial cellulose    synthase complex: Cmcax and CcpAx</strong></p></td>
−
<p class=MsoNormal style='line-height:150%'><b style='mso-bidi-font-weight:
+
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
−
normal'><span lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:
+
      </tr>
−
"Arial",sans-serif;color:black;background:#F1F0F0;mso-ansi-language:EN-US'>Team
+
</table>
−
</span></b><span class=SpellE><b style='mso-bidi-font-weight:normal'><span
+
</div>
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'>Botchan</span></b></span><b style='mso-bidi-font-weight:
+
−
normal'><span lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:
+
−
"Arial",sans-serif;mso-ansi-language:EN-US'> Lab Tokyo <span class=SpellE>japon</span><span
+
−
style='color:black;background:#F1F0F0'><o:p></o:p></span></span></b></p>
+
−
<![endif]><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-fareast-language:ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="Imagen_x0020_18"
+
−
o:spid="_x0000_i1027" type="#_x0000_t75" style='width:253.2pt;height:92.4pt;
+
−
visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="Collaborations_archivos/image028.png" o:title="" croptop="19536f"
+
−
  cropbottom="20761f" cropleft="21165f" cropright="5348f"/>
+
−
</v:shape><![endif]--><![if !vml]><img border=0 width=338 height=123
+
−
src="https://static.igem.org/mediawiki/2018/1/1f/T--Ecuador--survey4.png" v:shapes="Imagen_x0020_18"><![endif]></span><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif'><o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal style='line-height:150%'><span lang=ES-EC style='font-size:
+
−
12.0pt;line-height:150%;font-family:"Arial",sans-serif'>
+
−
<o:p>&nbsp;</o:p>
+
−
</span></p>
+
−
 
+
−
<p class=MsoNormal style='text-align:justify;line-height:150%'><b
+
−
style='mso-bidi-font-weight:normal'><span lang=EN-US style='font-size:12.0pt;
+
−
line-height:150%;font-family:"Arial",sans-serif;mso-ansi-language:EN-US'>Team <span
+
−
class=SpellE>Macquaire</span><o:p></o:p></span></b></p>
+
−
 
+
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
+
−
lang=ES-EC style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-fareast-language:ES-EC;mso-no-proof:yes'><!--[if gte vml 1]><v:shape id="Imagen_x0020_25"
+
−
o:spid="_x0000_i1025" type="#_x0000_t75" style='width:425.4pt;height:226.8pt;
+
−
visibility:visible;mso-wrap-style:square'>
+
−
<v:imagedata src="Collaborations_archivos/image032.png" o:title="T--Ecuador--survey6"/>
+
−
</v:shape><![endif]--><![if !vml]><img border=0 width=567 height=302
+
−
src="https://static.igem.org/mediawiki/2018/9/9b/T--Ecuador--survey6.png" v:shapes="Imagen_x0020_25"><![endif]></span><span
+
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'><o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'>&nbsp;</p>
+
−
 
+
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
+
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'>We thank all the teams involved in these collaborations
+
−
for giving us advice, for sharing their time with us, supporting our project
+
−
and making this experience more amusing. See you in Boston.<o:p></o:p></span></p>
+
−
 
+
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
+
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
−
 
+
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'><span
+
−
lang=EN-US style='font-size:12.0pt;line-height:150%;font-family:"Arial",sans-serif;
+
−
mso-ansi-language:EN-US'><o:p>&nbsp;</o:p></span></p>
+
−
 
+
−
</div>
+
−
 
+
−
<p class=MsoNormal align=center style='text-align:center;line-height:150%'>&nbsp;</p>
+
−
 
+
−
</div>
+
−
 
+
−
+
−
<div class="ec--p"></div>
+
−
<p>&nbsp;</p>
+
−
</div>
+
−
</div>
+
−
</div>
+
−
            </div>
+
 
</body>
 
</body>
−
 
+
<div class="ec--h4"><a name="RE">REFERENCES</a></div>
−
 
+
<div class="ec--p">
−
<script>
+
<div class="ec--p">Zhou,  N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. <a href="https://doi.org/10.1007/s00441-016-2403-0">https://doi.org/10.1007/s00441-016-2403-0</a></div>
−
var slider_Edu_Centers = new IdealImageSlider.Slider({
+
  </div>
−
selector: '#slider_Edu_Centers',
+
</div>
−
height: 500, // Required but can be set by CSS
+
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
−
interval: 3000
+
<script>
 +
$(document).ready(function(){
 +
$("#type").mouseenter(function(){
 +
    $(".textdis").show();
 
});
 
});
−
slider_Edu_Centers.addCaptions();
+
$("#label-neg-c").mouseenter(function(){
−
slider_Edu_Centers.start();
+
    $(".loc-neg-c").show();
−
+
−
</script>
+
−
+
−
<script>
+
−
var slider_Estonia = new IdealImageSlider.Slider({
+
−
selector: '#slider_Estonia',
+
−
height: 500, // Required but can be set by CSS
+
−
interval: 3000
+
 
});
 
});
−
slider_Estonia.addCaptions();
+
$("#label-pos-c").mouseenter(function(){
−
slider_Estonia.start();
+
    $(".loc-pos-c").show();
−
+
});
−
var slider_Valencia = new IdealImageSlider.Slider({
+
$("#label-td1").mouseenter(function(){
−
selector: '#slider_Valencia',
+
    $(".loc-td1").show();
−
height: 500, // Required but can be set by CSS
+
});
−
interval: 3000
+
$("#label-td2").mouseenter(function(){
 +
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Revision as of 01:33, 19 November 2018

iGEM ECUADOR

PARTS

Index

INTRODUCTION
The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed).
Fusion protein for bone and cartilage regeneration
Coding parts
BBa_K2804000
CBD cipA with a C-terminal linker
This part was design to try to improve the imperial college (2014) CBD cipA part BBa_K1321014, because the CBD N-terminal is where the protein binds to the cellulose and the linker could interfer with this interaction.
BBa_K2804006
Superfolder GFP with six-histidine tail
The adition of an histidine tail to part BBa_K1365020 was done because it allows the characterization of fusion proteins binding sites through atomic force microscopy, thanks to its affinity to the equipment nickel tip.
BBa_K2804002
Bone morphogenetic protein 2 (BMP2)
Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.1
Composite parts
BBa_K2804001
CBD cipA fused to BMP2 under the control of LacI promoter
Therapeutic protein coupled to the CBD whose expression is regulated by the presence of IPTG in the medium.
BBa_K2804004
CBD cipA fused to sfGFP
BBa_K2804005
CBD cipA fused to sfGFP under the control of LacI promoter
BBa_K2804014
CBD cipA fused to sfGFP
BBa_K2804015
CBD cipA fused to BMP2

Name

Description

Type

BBa_K2804000

CBD cipA with a C-terminal linker

Coding

BBa_K2804001

CBD cipA fused to BMP2 under the control of LacI promoter

Composite

BBa_K2804002

Bone morphogenetic protein 2 (BMP2)

Coding

BBa_K2804004

CBD cipA fused to sfGFP

Composite

BBa_K2804005

CBD cipA fused to sfGFP under the control of LacI promoter

Composite

BBa_K2804006

Superfolder GFP with six-histidine tail

Coding

BBa_K2804014

CBD cipA fused to sfGFP

Composite

BBa_K2804015

CBD cipA fused to BMP2

Composite

 

Fusion protein to provide elasticity to the biomaterial
Coding parts
BBa_K2804011
ELP V-150
BBa_K2804012
ELP V-150
Composite part
BBa_K2804013
CBD cipA fused to ELP_C5 under the control of LacI promoter

Name

Description

Type

BBa_K2804011

ELP V-150

Coding

BBa_K2804012

ELP_C5

Coding

BBa_K2804013

CBD cipA fused to ELP_C5 under the control of LacI promoter

Composite

 

Cellulose production module
Coding parts
BBa_K2804008
CmcAx: Endo-beta-1,4-glucanase ezyme
BBa_K2804009
CcpAx: Cellulose-complementing protein
Composite part
BBa_K2804010
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx

Name

Description

Type

BBa_K2804008

CmcAx: Endo-beta-1,4-glucanase ezyme

Coding

BBa_K2804009

CcpAx: Cellulose-complementing protein

Coding

BBa_K2804010

Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx

Composite
REFERENCES
Zhou, N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. https://doi.org/10.1007/s00441-016-2403-0
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UNIVERSIDAD DE LAS FUERZAS ARMADAS ESPE.
Ecuador,Sangolquí, Av. General Rumiñahui s/n y Ambato