Ser Archer (Talk | contribs) |
Ser Archer (Talk | contribs) |
||
Line 270: | Line 270: | ||
"<br><br>"+ | "<br><br>"+ | ||
"<h4>(1)The concentration of MTT (Biofoxx, Germany) is 5mg/ml. We prepare this by dissolving 0.5 g of MTT in 100 ml of phosphate buffer solution (PBS) or culture medium without phenol red. </h4>"+ | "<h4>(1)The concentration of MTT (Biofoxx, Germany) is 5mg/ml. We prepare this by dissolving 0.5 g of MTT in 100 ml of phosphate buffer solution (PBS) or culture medium without phenol red. </h4>"+ | ||
− | "<h4>(2)Filter the solution with 0.22 μm filter membrane to remove the bacteria, then store it at 4 ℃ (light should be avoided). Containers should be wrapped in aluminum foil during preparation and preservation.</h4>" | + | "<h4>(2)Filter the solution with 0.22 μm filter membrane to remove the bacteria, then store it at 4 ℃ (light should be avoided). Containers should be wrapped in aluminum foil during preparation and preservation.</h4>"+ |
"<br>"+ | "<br>"+ | ||
"<h2>Culturing Cells </h2>"+ | "<h2>Culturing Cells </h2>"+ |
Revision as of 14:56, 7 December 2018