We will be utilising iGEM registry parts sequenced by IDT to construct a biosynthetic
operon for expression in Escherichia coli. The operon will synthesise naringenin, a flavonoid that attracts nitrogen fixing bacteria.
The operon will contain the genes for four enzymes: 4 – Coumaryl ligase – 4CL (BBa_K1033001), Tyrosine ammonia lyase - TAL (BBa_K1033000),
Chalcone isomerase - CHI (BBa_K1497000) and Chalcone synthase - CHS (BBa_K1497001). Each of these parts contain a strong ribosome binding site
(BBa_B0034). This construct will be under the control of a T7 promoter (BBa_I712074) to observe its expression in E. coli as a proof of concept.
Once biosynthesis under the control of T7 is achieved, the construct will be tested under a constitutive promoter (BBa_J23100). Parts for biosynthesis
in the final chassis organism, root-colonising Pseudomonas fluorescens, will be constructed in the plasmid backbone pBSC1C3.
