Team:Newcastle/Notebook/Endophyte
Alternative Roots
Endophytic Chassis Notebook
NOTEBOOK
Developing Pseudomonas as a new endophytic chassis
Week Commencing 30/07
Development of our new endophytic chassis began on the 1st of August with the arrival of root colonising Pseudomonas sp. DSM25356 from DSMZ in Germany. The strain arrived in a glass ampoule and was inoculated onto tryptone soya agar plates using methods outlined by DSMZ.
The plates were incubated at 28 °C for 24 hours after which they were used to inoculate tryptone soya broth for initial growth characterisationWeek Commencing 06/08
The day began with a talk from Jon Marles-Wright about the importance of human practices and the impacts of our projects in the real world. This was followed by Dana Ofiteru who taught us about mathematical modeling of biological systems. In the afternoon, plant biologist Max Kapralov discussed using synthetic biology to improve the efficiency of photosynthesis. Finally, Rachel Armstrong (professor of experimental architecture) discussed the potential of microorganism in waste management systems and energy production in the built environment. Rachel also outlined political and economic issues surrounding synthetic biology.
