Team:Newcastle/Notebook/Endophyte
Alternative Roots
Endophytic Chassis Notebook
NOTEBOOK
Developing Pseudomonas as a new endophytic chassis
Week Beginning 20/07
Day 1 -07/06/18
Preliminary work began with the team developing agar-based germination methods, this was mainly due to the lack of plant research experience in the team. The team conceptualised growing Arabidopsis in microcentrifuge tubes within pipette-tip boxes.
The team planted their first set of 16 Arabidopsis seeds in 1% agar in a pipette-tip box placed on the lab windowsill. This would give an indication as to if these conditions were suitable for growth. After 7 days 12/16 seeds had germinated showing this method was appropriate.