Team:HebrewU/Design
Hebrew- Project
- Lab
- Human Practices
- Team
- Judging
Yeast Design
We began creating yeast strains, each with a single vector. After that, we had considered
utilizing the mat a/a system for creating strain with more than one vector, but as we found
our transformation method extremely efficient, we chose to execute secondary transformations
on yeast cells already contain a separate vector.
Parallel to these transformations, we engineered control strains that contained the empty vector, with
no extra enzymatic activity, but allowed for these stains to be grown on the same selective Drop Out media.
These strains were critical for creating useful control groups for our experiment design.
Our final yeast strain was known in the lab as D24 (Dehalogenase, 2,6,6 hydrolase + 2,3,3 dioxygenase
, and 44a dioxygenase) and has a corresponding control strain with 3 vectors allowing for growth on -Leu,
Trp, His Dropout media, but containing none of the enzyme from the TCDD degradation pathway.
Further Reading: