Team:Tufts/Attributions

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Attributions

Tufts iGEM, or Tufts Synthetic Biology Team, is and has always been a student-run endeavor. We are proud of our independence, but we could not have accomplished so much without help. Thank you to everyone who supported us along the way!

Team Member Contributions

Anna: Anna was responsible as iGEM science chair for overseeing all lab protocols. Anna took charge of connecting with the Nair lab graduate students for protocol guidance and ran the interlab initiative. She also set up the public outreach by collaborating with a local summer camp to teach genetics and synthetic biology classes there.

Aiden: Aiden was responsible as iGEM financial chair for ordering and organizing materials and kits. Aiden troubleshooted the gel electrophoresis and PCR processes, as well as ensured access to the lab facilities. Initial protocols were designed by Aiden based on manufacturer instructions.

Mina: Mina contributed by running experimental portions of the project with Anna and Aiden. Mina made the website HTML.

Charlotte: Charlotte produced the summer camp educational video for outreach.

Val: Val supported the iGEM team by conducting useful research during the summer project and conducting lab tests.

Kenny: Kenny was responsible as iGEM administrative chair for organizing school-year meetings for research planning and securing university funding.

Donations

Thank you to the Tufts Community Union Senate for funding our team's iGEM competition registration fees.

Thank you to Tufts University President Tony Monaco for providing stipends for campus housing so that students could stay for summer research.

Mentors

Thank you to our graduated seniors, Lucas Brown, Adam Rayfield, Dani Burton, and Hayley Carabello. Thank you to our graduate student mentors, Jessica Stieglitz and Joe Bober. Thank you to the Nair Lab for advising. Thank you to Tufts University and Bruce Panilaitis for Science and Engineering Center lab facility access.

Inspirations & Project Basis

BU iGEM: We were inspired by the use of toeholds in the BU iGEM 2017 project. We incorporated toehold switches into our Cas9 system.

Alexander Green's 2014 paper on toehold switches was the basis for the Tufts iGEM team's theoretical toehold understanding. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression." Cell 159.4 (2014): 925-939.

• Thanks and acknowledgements for all other people involved in helping make a successful iGEM team

Thank you iGEM Foundation for providing our team with a great experience!