Team:HUST-China/Parts Overview
Parts Overview
As mentioned in the project description and result, our three different circuits in three different kinds of bacteria well supported our project and each of them contributes a lot to the biobricks. This year, we designed, constructed and verified 25 parts, and 21 of them were submitted. There are composite partsproducing enzyme to transform different carbon source to lactate and transform out of the bacteria. We completely collected the parts from our project and well documented them with our test data.
| Part Number | Name | Description |
|---|---|---|
| BBa_K2533000 | RBS-ldhD-RBS-lldP | dhD can produce lactate, and lldP can release it from cytoplasm to the environment |
| BBa_K2533002 | RBS-ldhDnARSdR-RBS-lldP | ldhDnARSdR can produce lactate, and lldP can release it from cytoplasm to the environment. |
| BBa_K2533003 | RBS-ldhDARSdR-RBS-lldP | ldhDARSdR can produce lactate, and lldP can release it from cytoplasm to the environment. |
| BBa_K2533014 | RBS-TH-RBS-gldA-RBS-LLDP | produce and release lactate |
| BBa_K2533016 | RBS-ldhD-FLAG-lldP-his tag | ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533016 | RBS-ldhD-FLAG-lldP-his tag | ldhD can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533017 | RBS-ldhDC-FLAG-lldP-his tag | ldhDC can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533018 | RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag | ldhDnARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533019 | RBS-ldhDARSdR-FLAG-RBS-lldP-his tag | ldhDARSdR can produce lactate, lldP can release it from cytoplasm to the environment, and tags will show the expression of the protein. |
| BBa_K2533030 | RBS-dld | It encodes D-lactate dehydrogenase. |
| BBa_K2533031 | RBS-lldEFG | produce L-lactate dehydrogenase complex protein. |
| BBa_K2533032 | RBS-dld-TT-Ptac-RBS-lldEFG | It encodes FAD-dependent D-lactate dehydrogenase and L-lactate dehydrogenase complex protein. |
| BBa_K2533033 | RBS-gapA | It encodes glyceraldehyde-3-phosphate dehydrogenase. |
| BBa_K2533034 | RBS-mdh | It encodes NAD dependent malate dehydrogenase. |
| BBa_K2533035 | RBS-gapA-RBS-mdh-TT | It encodes glyceraldehyde-3-phosphate dehydrogenase and NAD dependent malate dehydrogenase. |
| BBa_K2533036 | RBS-pflB | It encodes pyruvate formate-lyase. |
| BBa_K2533037 | RBS-fdh | It encodes formate dehydrogenase cytochrome b. |
| BBa_K2533038 | RBS-pflB-RBS-fdh | It encodes pyruvate formate-lyase and formate. |
| BBa_K2533039 | RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh | It encodes glyceraldehyde-3-phosphate dehydrogenase, NAD dependent malate dehydrogenase, pyruvate formate-lyase and formate dehydrogenase cytochrome b. |
| BBa_K2533045 | RBS-mleS | It encodes malate dehydrogenase. |
| BBa_K2533046 | RBS-lldP | It encodes L-lactate permease. |
| BBa_K2533047 | RBS-ldhA | It encodes D-lactate dehydrogenase. |
| BBa_K2533048 | RBS-mleS-RBS-lldP | It encodes malate dehydrogenase & L-lactate permease. |
| BBa_K2533049 | RBS-lldP-RBS-ldhA | It encodes L-lactate permease & D-lactate dehydrogenase. |
| BBa_K2533050 | mleS-lldp-ldhA | It encodes malate dehydrogenase & L-lactate permease & D-lactate dehydrogenase. |
| BBa_K2533051 | mleS-lldP-TT | It encodes malate dehydrogenase & L-lactate permease. |
| BBa_K2533052 | RBS-lldP-RBS-ldhA-TT | Product and transport of lactic acid. |
| BBa_K2533053 | RBS-mleS-RBS-lldP-RBS-ldhA-TT | Product and transport of lactic acid. |
| BBa_K2533054 | RBS-ldhA-RBS-lldP | Product and transport of lactic acid. |
| BBa_K2533055 | RBS-ldhA-RBS-lldP-TT | Product and transport of lactic acid. |
