Team:SCU-China/attribution

Team:SCU-China - 2018

CRISProgrammer in Indigo

Sincerely thanks to all those who have helped us over this year, not only for helping on the project, but also for experiencing an unforgettable journey together. Here we describe what we have done by ourselves this year and what was done by others in detail.
First, we want to thanks our PIs and instructor, as well as all the teachers who provided great support to our project and the promotion of synthetic biology.

1. PI and Instructor

Dr. Nianhui Zhang

, team primary PI, advisor of project defense, advisor of project defense, judge of ACMA, provided laboratory for us and pay for expenses on account.

Dr. Chuanfang Wu

，team secondary PI, teacher of Molecular Biology in winter training, advisor of project defense, judge of ACMA.

Hongbin Yu

, instructor of our team, advisor of project defense, provide us with experimental support.

2. Other Teachers

Yunzi Luo

, advisor of project defense, provided great help to us during the interview of Human Practice

Ying Tong

, teacher of Commonly Used Experimental Methods in Modern Biology and Case Study in winter training, advisor of project defense, judge of ACMA

Yang Zhang

, teacher of Plant Synthetic Biology in winter training

Jian Zhao

, teacher of Microbiology Experimental Skills and Laboratory Safety, advisor of project defense, judge of ACMA

Jia Fan

, advisor of project defense, judge of ACMA

Zhibin Liu

, advisor of project defense

Yujun Zhang

, gave us some advice during our project processing.

Xiaoping Wan

, judge of ACMA, provided help related to hold the ACMA

Han Kang

, teacher who is in charge of the plate reader in Life Science College, manipulated the instruments.

Fei Huang

, advisor of project defense, provided great help to us during the interview of Human Practice

Chuan Wang

, associate professor form Chengdu University of Technology, advisor of project defense

Rongwu Yang

, teacher from Nanjing University, helped us deal with a question during project processing

Haiyan Liu

, teacher from University of Science and Technology of China, generously shared their plasmid with dCas9 in it.

Changhao Bi

, teacher from Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, generously shared their plasmid used in E. coli knock-in.

Secondly, we want to show you the division of labor in this year’s project, both team roster members and reversed team members. Some other students who contributed to our project are also listed here.

1. Students in Team Roster

Fuqiang Yang

, team leader, one of the group leaders in indigo part (BGL-A synthesis), speaker of Synthetic Biology Discussion in winter training, participated the collaboration part with CDHSU, NWU-China, wrote the related wiki content.

Zhiwei Luo

, vice team leader, one of the group leaders in CRISProgrammer part, designed different sgRNA targeting promoters and coding sequence, verified their function, wrote the related wiki content.

Wei Lin

, vice team leader, one of the group leaders in CRISPR part, designed the bi-stable switch, verified its function, speaker in CCiC, wrote the related wiki content.

Yibing Tao

, one of group leaders in dCas protein synthesis and verification, helped in CRISProgrammer part, group leader of InterLab, wrote the related wiki

Junjie Xu

, designed and did experiments related to CRISProgrammer part

Yuliang Xiang

, came up the idea of applicating the CRISProgrammer to indigo synthesis, one of the group leaders of indigo part (FMO synthesis)

Haoyang Cheng

, came up the idea of CRISProgrammer, one of group leaders in dCas protein synthesis and verification, speaker in winter training, in charge of the reagent purchase this year, wrote the related wiki

Mingyi Lv

, one of the group leaders of indigo part (UGT synthesis), in charge of lab safety this year

Tianmeng Wu

, group leader of modeling and wiki, in charge of uploading files, safety form, judge form and wiki content.

Ziru Wang

, did experiments related to dCas protein verification, InterLab

Changhe Li

, did experiments related to circuit construction and verification, wrote the ACMA guide

Yanling Zhong

, did experiments related to circuit construction and verification

Yumei Yang

, did experiments related to circuit construction and verification, wrote the notebook and wiki related to circuit construction

Caiqin Wang

, did experiments related to circuit construction and verification, wrote the related wiki

Tianyi Zhang

, did experiments related to circuit construction and verification, helped in InterLab study, wrote the related wiki and notebook

Chenming Zhang

, did experiments related to BGL-A synthesis, leader of human practice, wrote the related notebook, revised the wiki of human practice

Tian Liu

, did experiments related to BGL-A synthesis and collaboration part with NWU-China, wrote the related notebook, revised the wiki of human practice

Qinling Qiu

, did experiments related to FMO synthesis and circuit construction and verification, wrote the notebook and wiki related to circuit construction

Yuanhan Yang

, did experiments related to UGT synthesis, helped in logic circuit construction, wrote the related notebook

Mucun Hou

, did experiments related bi-stable switch, wrote the related wiki and notebook

Yuqing Zhao

, did experiments related to BGL-A synthesis and designed the poster for CCiC, helped in modeling, wrote the related wiki

Hanzhou Zhu

, did experiments related to knock-in system, wrote the related wiki and notebook

Minyue Bao

, did experiments related to circuit construction and verification, helped in InterLab study

Linfeng Li

, did the experiments related to UGT synthesis, wrote the related notebook

Xiaolong Liu

, did experiments related to circuit construction and verification

Mingxuan Bai

, did experiments related to FMO synthesis

2. Reserved Team Member

Yaxin Luo

, helped in FMO expression and did great help to human practice, wrote the relate wiki and ACMA guide

Yanling Yang

, did great help to human practice, wrote the wiki related to CCiC and some other interviews.

Hao Zhou

, helped in preparation of experiments in summer.

Fengxi Yu

, wrote the wiki related to ACMA and some other interviews.

Xinran Shen

, helped in FMO expression and preparation of experiments in summer

Zhenyi Wang

, helped in preparation of experiments in summer

Yuxi Liu

, wrote the wiki related to CCiC

Qihan Jin

, wrote the wiki related to modeling

Yi Wu

, helped in preparation of experiments in summer

Yitao Ke

, wrote the wiki related to meeting-up

LinFeng Mao

, helped in preparation of experiments in summer

Xi Chen

Yifan Li

Xiaokun Ren

3. Other Students

Junyan Zhu

, former tram leader of SCU-China, talked with us and gave us some suggestions

Jiusi Guo

, generously provided poster and leaflet for us during recruitment for 2018 SCU-China, helped us contacting the instruments in Sichuan University Huaxi campus.

Tingting Zhao

, art designer of our team, designed the team uniform, banner and other artistic elements in wiki, poster and presentation

Jia Yan

, helped in the wiki of communication with Chengdu University of Information Technology

Hao Zhou

, helped in the wiki related to collaboration of CDHSU

Finally, we are really appreciated for all the help from students in different teams, especially peers from SDU-China, XMU-China, UESTC, CSU-China, TJU-China, NWU-China and HUST. Thank you for all the help during the whole project processing.

About Us

We started to prepare for 2018 iGEM a few weeks after the 2017 iGEM. Team leader, vice team leader and group leaders and reserved team members were determined at that time. We held a short training lasting for almost one week at the beginning of winter holiday with the help of our instructors and the college. Brainstorms were held to come up with our new project during the training. Two parts of our project, CRISPR system and indigo synthesis namely, were presented at this time. During the Spring semester, we held project analysis for several times to learn from other teams. ACMA was successfully held as well. General project frame was determined in May with the help of our teachers, advisors, as well as previous iGEMers. Experiments were done from July to October.
Details of our winter training will also be introduced to you here.
Winter training was held a few days after Fall semester. In a training lasting for one week, teachers and speakers will present the basic ideas and knowledge related to molecular biology and synthetic biology. It was our great honor to invite professor Yang Zhang to address several talks related to plant synthetic biology. Laboratory safety was also be emphasized. Considering the short time of winter training, we didn’t prepare experiments as before this year. Timetable of this year’s winter training is shown in figure 1. Students in winter training mainly are reserved team member selected before. The training is not obligated to reserved team members. Though our materials are not available to all students in our college, people who are not in our reserved team but want to participate the training is welcomed. Training material is totally available to them, too. Students will be grouped before the training, including people who are not in our reserved team. Brainstorms were held every night based on groups. On the last day, we will hold project defense to show our primary ideas about project. During this year’s final defense, we held a live on www.bilibili.com and invited team members from Shandong to watch it.

Figure 1. Timetable

From Marth to June, we held group meeting almost every week to study the outstanding projects in 2017 iGEM. Students were required to do presentations in the group meetings to help themselves understand the design and experiments, and A comprehensive test was used to determine the team member and reversed team member in this year. Experiments were started in July and continued to the last day before wiki freeze, with human practice and collaboration keeping in line with it.
Thanks to the attempts at standardizing team structure did by former SCU-China iGEMers and the help of our instructors and college, we generally form an inheriting structure, though it may cause that we have many team members every year. After a whole year’s competition, several junior students become group leaders who take control of the team organization, experiments and so on. Most sophomores in the team are developed form the reversed team member in the last year and they become the main part of our team if they determine to remain and pass the test mentioned above. Freshmen were encouraged to participated in winter training and were required to attend the group meetings. Experiments were mainly completed by juniors and sophomores while human practice was mainly accomplished by freshmen under the leadership of senior grades students.
Nowadays, SCU-China is not just an iGEM team but also a platform for students in Sichuan University to practice the experimental skills, to learn and understand the synthetic biology, to work together and to complete the project designed by ourselves. With the encourage of college, our education parts of human practice become easier to accomplish (see human practice). By integrating the competition into undergraduate education of college, we receive the great support from our Life Science College every year and we sincerely hope such a kind of team structure organization idea would help new team to find their suitable road, especially teams who met obstacles on dealing with financial or other problems with their colleges.

Team Photo