Judgin Form Text
We proudly present the Marburg Collection, a novel golden-gate-based toolbox containing various parts that are compatible with the PhytoBrick system and MoClo. Compared to other bacterial toolboxes, the Marburg Collection shines with superior flexibility. We overcame the rigid paradigm of plasmid construction - thinking in fixed backbone and insert categories - by achieving complete de novo assembly of plasmids. 36 connectors facilitate flexible cloning of multigene constructs and even allow for the inversion of individual transcription units. Additionally, our connectors function as insulators to avoid undesired crosstalk. The Marburg Collection contains 122 parts in total, including: inducible promoters, reporters, fluorescence and epitope tags, oris, resistance cassettes and genome engineering tools. To increase the value of the Marburg Collection, we additionally provide detailed experimental characterization for V. natriegens and a supportive software. We aspire availability of our toolbox for future iGEM teams to empower accelerated progression in their ambitious projects.(Range:BBa_K2560001 - BBa_K2560305)Basic building blocks like promoters or terminators are stored in level 0 plasmids. Parts from each category of our collection can be chosen to built level 1 plasmids harboring a single transcription unit. Up to five transcription units can be assembled into a level 2 plasmid.
Partcollection
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560008 | B0034 | BBa_K2560002 (pSB1C3 derivative) |
| K2560010 | B0032 | BBa_K2560002 (pSB1C3 derivative) |
| K2560013 | B0031 | BBa_K2560002 (pSB1C3 derivative) |
| K2560016 | B0030 | BBa_K2560002 (pSB1C3 derivative) |
| K2560084 | RBS Dummy | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560033 | E1010 | BBa_K2560002 (pSB1C3 derivative) |
| K2560041 | E0030 | BBa_K2560002 (pSB1C3 derivative) |
| K2560042 | sfGFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560043 | sfGFP Vn | BBa_K2560002 (pSB1C3 derivative) |
| K2560047 | Cas9 | BBa_K2560002 (pSB1C3 derivative) |
| K2560051 | Lux Operon | BBa_K2560002 (pSB1C3 derivative) |
| K2560054 | dCas9 | BBa_K2560002 (pSB1C3 derivative) |
| K2560082 | CDS Dummy | BBa_K2560002 (pSB1C3 derivative) |
| K2560114 | LacZ Alpha | BBa_K2560002 (pSB1C3 derivative) |
| K2560128 | K6600004 | BBa_K2560002 (pSB1C3 derivative) |
| K2560135 | SXT Beta | BBa_K2560002 (pSB1C3 derivative) |
| K2560268 | tfox Vc | BBa_K2560002 (pSB1C3 derivative) |
| K2560269 | tfox Vn | BBa_K2560002 (pSB1C3 derivative) |
| K2560270 | SXT | BBa_K2560002 (pSB1C3 derivative) |
| K2560272 | flp recombinase | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560034 | B0010 | BBa_K2560002 (pSB1C3 derivative) |
| K2560035 | B0015 | BBa_K2560002 (pSB1C3 derivative) |
| K2560081 | Terminator Dummy | BBa_K2560002 (pSB1C3 derivative) |
| K2560089 | B1002 | BBa_K2560002 (pSB1C3 derivative) |
| K2560091 | B0003 | BBa_K2560002 (pSB1C3 derivative) |
| K2560093 | B0006 | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560036 | ColE1 | BBa_K2560002 (pSB1C3 derivative) |
| K2560037 | pMB1 | BBa_K2560002 (pSB1C3 derivative) |
| K2560046 | p15A | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560048 | Cam. Res. RFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560056 | Kan. Res.(pSB3K3) RFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560057 | Kan. Res. (pSB3K3) GFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560058 | Tet. Res. (pSB3T5) RFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560059 | Tet. Res. (pSB3T5) GFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560125 | Carb. Res. RFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560126 | Carb. Res. GFP | BBa_K2560002 (pSB1C3 derivative) |
| K2560127 | Carb. Res. into BBa_K2560002 | BBa_K2560002 (pSB1C3 derivative) |
| K2560132 | Cam. Res. into BBa_K2560002 | BBa_K2560002 (pSB1C3 derivative) |
| K2560133 | Kan. Res. into BBa_K2560002 | BBa_K2560002 (pSB1C3 derivative) |
| K2560134 | Tet. Res. into BBa_K2560002 | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560060 | E1010 4x | BBa_K2560002 (pSB1C3 derivative) |
| K2560063 | sfGFP 4x | BBa_K2560002 (pSB1C3 derivative) |
| K2560085 | His Tag 4x | BBa_K2560002 (pSB1C3 derivative) |
| K2560087 | FLAG Tag 4x | BBa_K2560002 (pSB1C3 derivative) |
| K2560129 | K660004 4x | BBa_K2560002 (pSB1C3 derivative) |
| K2560257 | Streptag 4x | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560061 | E1010 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560062 | B0015 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560064 | sfGFP 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560086 | His Tag 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560088 | FLAG Tag 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560090 | B1002 5b | BBa_K2560002 (pSB1C3 derivative) |
| K2560092 | B1003 5b | BBa_K2560002 (pSB1C3 derivative) |
| K2560094 | B1006 5b | BBa_K2560002 (pSB1C3 derivative) |
| K2560117 | I11012 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560118 | M0050 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560119 | M0051 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560130 | K660004 5a | BBa_K2560002 (pSB1C3 derivative) |
| K2560258 | Streptag 5a | BBa_K2560002 (pSB1C3 derivative) |
| BioBrick | Name | Plasmid |
|---|---|---|
| K2560001 | Entry Vector with RFP Dropout | BBa_K2560002 (pSB1C3 derivative) |
| K2560002 | Entry Vector with GFP Dropout | BBa_K2560002 (pSB1C3 derivative) |
| K2560005 | Resistance Entry Vector with RFP Dropout | Falsch |
| K2560006 | Resistance Entry Vector with GFP Dropout | Falsch |
| K2560305 | gRNA Entry Vector with GFP Dropout | BBa_K2560002 (pSB1C3 derivative) |