Team:HUST-China/Modeling overview
Modeling overview
1. Overview
This year, our job can mainly divided into three parts: Comparison of Rhodopseudomonas and Synechocystis, sort of three genes pathway in Rhodopseudomonas, and intelligent device software.
2. Comparison between PSB
In the past, people use Synechocystis to provide energy for electricity producing bacteria. Because it’s efficient and easier to control. But Shewanella species are heterotrophic facultative anaerobes, and produce electricity only in anaerobic environment (Justin et al.2009). So we think Rhodopseudomonas may be better because it’s cycle photophosphorylation doesn’t produce oxygen. But Rhodopseudomonas is not as efficient as Synechocystis. So we use computer simulation to find which is better.
3. Sort of three genes
In Rhodopseudomonas, the sequence of genes in plasmid influence them expression ability. (Shou-Chen.2012) We have three gene to transfer. So we should find out which gene is most important, and we sort three genes by their importance.
4. Intelligent device software
Using a new multi-culture bacteria system means we should use a new fermentation strategy. So we use algorithm to know the system’s situation and decide how to control the device, letting system work more efficiently and steadily.
Reference
[1] Justin L. Burns, Thomas J. DiChristina. 2009. Anaerobic Respiration of Elemental Sulfur and Thiosulfate by Shewanella oneidensis MR-1 Requires psrA, a Homolog of the phsA Gene of Salmonella enterica Serovar Typhimurium LT2. American Society for Microbiology Journals
[2] 2.Shou-Chen Lo. 2012. Enhancement of Hydrogen Production and Carbon Fixation in Purple Nonsulfur Bacterium by Synthetic Biology. Ph.D. Dissertation
