Team:IIT Delhi/Basic Part

iGEM IIT Delhi

Notebook
Lab Records Medal Criteria
Team
Members Attributions
Collaboration
Human Practices
Human Practices Safety Public Engagement
Modelling
Parts
Basic Parts Composite Parts
Results
Demonstrate Interlab
Project
Overview Design

 S. No
Part Name 
BioBrick 
Description 
Part Type 
1

BBa_K2814001

pLTL 
Lac-Tet-Lac Hybrid Promoter
Promoter 
2 

BBa_K2814007

sfGFP_ssrA 
Reporter gene (codon-optimised for E. coli) 
Coding Sequence 
3 

BBa_K2814008

rrnB T1 Terminator + T7Te Terminator 
Double Transcriptional Terminator 
Terminator 
4 

BBa_K2814009

mKate2 
Reporter gene (codon-optimised for E. coli)  
Coding Sequence 
5 

BBa_K2814010

rrnB T1 Terminator 
Transcriptional Terminator
Terminator 
6 

BBa_K2814011

attP TP901-1 
Attachment site for TP901-1 Integrase 
DNA 
7 

BBa_K2814012

BxbI Integrase + ssrA(LVA) deg tag 
CDS for Integrase Bxb1  
Coding Sequence 
8 

BBa_K2814013

attB TP901-1 
Attachment site for TP901-1 Integrase 
DNA 
9 

BBa_K2814014 

complement of B0034, RBS on the antisense strand 
Ribosome Binding Site 
RBS 
10 

BBa_K2814015

pLac(lambda) hybrid 
Lac-Lambda Hybrid Promoter 
Promoter
11 

BBa_K2814017

attP Bxb1 
Attachment site for Bxb1 Integrase 
DNA 
12 

BBa_K2814018

attB Bxb1 
Attachment site for Bxb1 Integrase 
DNA 
13 

BBa_K2814019

P7 Promoter 
Constitutive P7 promoter 
Promoter
14

BBa_K2814021 

BxbI-Xis  + ssrA(LVA) deg tag 
CDS for Recombinase Directionality Factor for Integrase Bxb1 
Coding Sequence 
15

BBa_K2814022 

attB BxbI (reverse orientation) 
Attachment site for Bxb1 Integrase (Reverse) 
DNA 
16
BBa_K2814025

 

TP901-1 Integrase 

CDS for Integrase TP901-1
Coding Sequence

We faced some problems during our InterLab characterization, our absorbance at 600nm for silica beads at high dilutions didn't follow the trend. Upon which, we discussed the problem on All India iGEM group, where the teams gave us multiple suggestions and shared their protocols by which we were able to obtain our results as deisred by iGEM. Hence, we would like to express our gratitude towards the Indian iGEM teams.

Contact us

Address

Undergraduate Laboratory
Department of Biotechnology and Biochemical Engineering, IIT Delhi