Team:SHSBNU China/Parts
Parts
I. Overview
We have finished constructing all the parts(BBa_K2684000- BBa_K2684006) which have all been sequenced and submitted to the iGEM parts registry
| Name | Description | Function |
| BBa_K52684000 | CotA laccase of B. subtilis | CotA of B. subtilis is a copper-dependet laccase, which can catalyze the oxidation of board range of synthetic dyes. |
| BBa_K52684001 | PelB-CotA | PelB signal peptide linking with CotA to transfer CotA out of the bacteria. |
| BBa_K52684002 | PhoA-CotA | PhoA signal peptide linking with CotA to transfer CotA out of the bacteria. |
| BBa_K52684003 | OmpA-CotA | OmpA signal peptide linking with CotA to transfer CotA out of the bacteria. |
| BBa_K52684004 | SpyCatcher-sfGFP | SfGFP fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag.This part is used to test our immobilization strategy of CotA. |
| BBa_K52684005 | SpyCatcher-CotA | CotA fused to the SpyCatcher domain can be covalently attached onto the biofilm displaying SpyTag. |
| BBa_K52684006 | CsgA-SpyTag | CsgA is a major subunit of the biofilm of E. coli(1)(2). A SpyTag is fused to CsgA so that CotA laccase can be fixed on biofilm through SpyTag-SpyCatcher chemistry. |
a. Improve Previous Parts
We improved partBBa_K1583000 by adding a sequence of SpyTag in order to fix CotA laccase onto the biofilm.
We improved partBBa_K1336002 by eliminating its EcoRI cutting site.II. References
(1). Wang, X, et al. “Programming Cells for Dynamic Assembly of Inorganic Nano-Objects with Spatiotemporal Control.” Advanced Materials (Deerfield Beach, Fla.)., U.S. National Library of Medicine, Apr. 2018, www.ncbi.nlm.nih.gov/pubmed/29516606.
(2). Chen, Allen Y., et al. “Synthesis and Patterning of Tunable Multiscale Materials with Engineered Cells .” Nature Materials, U.S. National Library of Medicine, May 2014, www.ncbi.nlm.nih.gov/pmc/articles/PMC4063449/.