Team:Marburg

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  • Best New Basic Part
  • Best New Composite Part
  • Best Part Collection
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• Judging Form

Establishing Vibrio natriegens as the new chassis organism for synthetic biology

Strain Engineering

We established three new strains derived from the original wildtype strain as chassis organisms for cloning, protein expression and protein interaction studies.

Part Collection

We created the Marburg Collection, a highly flexible golden-gate based cloning toolbox consisting of 123 individual parts. Our novel measurement workflow was applied to obtain highly reproducible data on the behavior of our parts in V. natriegens

Metabolic Engineering

We developed a workflow for accelerating metabolic engineering. As a proof of concept we established the first synthetic pathway in Vibrio natriegens to produce the platform chemical 3-hydroxypropionic acid.

Interlab

To collaborate with other teams and spread the word to other scientists on Vibrio natriegens' advantages we had teams from all over Europe conduct a growth curve or a InterLab inspired experiment.

Accessible Science

This Wiki is designed to be accessible to/for everyone! For this years Human Practices, iGEM Marburg decided to partner up with the BLISTA again. The BLISTA is the only Highschool for visually impaired students in Europe. As part of our project we (and other teams) designed a barrier-free website to take this step to remove barriers in communication!

Medal Criteria

Bronze

We fulfilled all bronze medal criteria Our team registered for iGEM, we had a challenging but great iGEM season and we are really looking forward to attend the Giant Jambore

Deliverables

We already finalized our Wiki and Judging Form. We will hold our presentation at the Giant Jamboree in Boston and we expect exciting discussions at our poster.

Attributions

SOur project was only possible with a great team and we also want to thank people, institutions and companies who helped with financial and infrastructural support or scientific advice, and we are grateful to honor them on our attributions page.

Characterization

We participated in the fifth iGEM InterLab measurement study, submitted our data in time and we are happy that our results were accepted by the measurement committee.

Silver

We are certain that we completed all silver medal criteria

Validated Part

: We submitted BBa_K2560069 as a new basic parts. This part fulfills all formal requirements and was submitted to the registry. BBa_K2560069 is a 5’ Connector which was newly designed by us. We validated its function by showing that it can reduce crosstalk from backbone features 14 fold.

Collaboration:

We strengthened the iGEM community in Germany by hosting the German iGEM Meetup. We planned and realized the Vibrigens InterLab study with eleven participating teams.

Human Practices

We got into contact with the BLISTA as part of our local community to open our project for everyone and we achieved to present our project in an accessible manner for the visually impaired.

Gold

We hope that we can convince you that we expanded on our Human practives, improved a part, modeld our project and that we can demonstrate that our engineered system is functional.

Integrated Human Practices

We integrated valuable input from society, academia and industry into the design and execution of our project by starting a wiki collaboration and implementing the suggestions of stakeholders into the design of our strains.

Improve a Previous Part

We improved BBa_P10500 by replacing the dropout with a sfGFP cassette, resulting in BBa_K2560002.

Model Your Project

We used a kinetic model to predict the metabolic pathway that yields optimal production of 3-HPA.

Demonstration of Your Work

We succeeded in all major parts of our project. We established genome engineering methods, created a toolbox and showed production of 3-HPA

Special Prizes

We applied for the following special awards

Integrated Human Practices

For including inputs from all possible fields into every major aspect of our project.

Education and Public Engagement

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Modeling

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Measurment

The amount of acquired data for our parts was only possible with a measurement workflow tailored to V. natriegens

Best New Basic Part

We provide BBa_K2560268 a part that enables highly efficient transformation using natural transformation.

Best New Composite Part

We propose BBa_K2560272 which makes natural transformation inducible

Best Part Collection

The Marburg Collection contains 123 parts and represents the most flexible bacterial cloning toolbox

Results	Achieved
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B. Marchal