Team:HUST-China/Composite part
Composite part
Part1: Photosynthetic microorganism system: optical energy→chemical energy
1.1 Synechocystis sp. PCC 6803(cyanobacteria)
| Name | Description |
|---|---|
| BBa_K2533000 | RBS-ldhD-RBS-lldP |
| BBa_K2533001 | RBS-ldhDC-RBS-lldP |
| BBa_K2533002 | RBS-ldhDnARSdR-lldP |
| BBa_K2533003 | RBS-ldhDARSdR-lldP |
| BBa_K2533014 | RBS-TH-RBS-gldA-RBS-LLDP |
| BBa_K2533016 | RBS-ldhD-FLAG-RBS-lldP-his tag |
| BBa_K2533017 | RBS-ldhDC-FLAG-RBS-lldP-his tag |
| BBa_K2533018 | RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag |
| BBa_K2533019 | RBS-ldhDARSdR-FLAG-RBS-lldP-his tag |
BBa_K2533000, BBa_K2533001, BBa_K2533002, BBa_K2533003, BBa_K2533014
BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019
1.2. Rhodopseudomonas palustris
| Name | Description |
|---|---|
| BBa_K2533045 | RBS-mleS |
| BBa_K2533046 | RBS-lldP |
| BBa_K2533047 | RBS-ldhA |
| BBa_K2533048 | RBS-mleS-RBS-lldP |
| BBa_K2533049 | RBS-lldP-RBS-ldhA |
| BBa_K2533050 | mleS-lldp-ldhA |
| BBa_K2533051 | mleS-lldP-TT |
| BBa_K2533052 | RBS-lldP-RBS-ldhA-TT |
| BBa_K2533053 | RBS-mleS-RBS-lldP-RBS-ldhA-TT |
| BBa_K2533054 | RBS-ldhA-RBS-lldP |
| BBa_K2533055 | RBS-ldhA-RBS-lldP-TT |
BBa_K2533050, BBa_K2533048, BBa_K2533054, BBa_K2533049
Our photosynthetic microorganism system is consist of Synechocystis sp. PCC 6803(cyanobacteria) and Rhodopseudomonas palustris. They are all modified to produce lactate to improve the electrogenic efficiency of Shewanella(S.oneidensis).
Part2: Electrogenic microorganism system: chemical energy→electric energy
2.1. Shewanella(S.oneidensis)
| Name | Description |
|---|---|
| BBa_K2533030 | RBS-dld |
| BBa_K2533031 | RBS-lldEFG |
| BBa_K2533032 | RBS-dld-TT-Ptac-RBS-lldEFG |
| BBa_K2533033 | RBS-gapA |
| BBa_K2533034 | RBS-mdh |
| BBa_K2533035 | RBS-gapA-RBS-mdh-TT |
| BBa_K2533036 | RBS-pflB |
| BBa_K2533037 | RBS-fdh |
| BBa_K2533038 | RBS-pflB-RBS-fdh |
| BBa_K2533039 | RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh |
| BBa_K2533056 | PlacIq-lacI-Ptac-RBS-gapA |
| BBa_K2533057 | PlacIq-lacI-Ptac-RBS-mdh |
BBa_K2533030, BBa_K2533031, BBa_K2533032, BBa_K2533033, BBa_K2533034, BBa_K2533036, BBa_K2533037
BBa_K2533035, BBa_K2533038, BBa_K2533039
Our MFC produces electricity by Shewanella(S.oneidensis) through transfer chemical energy into electric energy. As a result, we design these parts to strengthen the ability of Shewanella(S.oneidensis) to absorb lactate，with an intention to boost the efficiency of electrogenic microorganism system.
