Team:HSHL/Results
Results
The synthesis of the HMA3 gene which was needed for this experiment was done by IDT. A gene with the entire sequence was synthesized as well as a gene that was divided into four fragments. First, Gen and Vector were prepared for ligation by cutting both components using the enzymes EcoRI and PstI. The vector, called pSB1C3 was then dephosphorilised by Shrimp Alkaline Phosphatase and the insert, the gene HMA3, phosphorilised by T4 Polynucleotide Kinase. After that gel electrophoresis and the Wizard SV Gel and PCR Clean- Up System of the company Promega were used to purify both components. Below you can see the result of the gel electrophoresis purification of the four fragments.
The IDT 2- log DNA ladder was used as a control. The following figure shows the Ladder schematically.
By evaluating the gel electrophoresis results with the help of the ladder, the following quantities results are determined for the four fragments.
| Fragment No. | Length [bp] |
|---|---|
| 1 | 614 |
| 2 | 593 |
| 3 | 556 |
| 4 | 885 |
Result of gel electrophoresis purification of the four fragments
Representation of the IDT 2-log ladder and the length of its bands
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