Team:Marburg/Experiments

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Experiments

Labbooks

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• Subgroup Strain Engineering - General experiments

• Subgroup Strain Engineering - dns experiments

• Subgroup Strain Engineering - VibriClone

• Subgroup Strain Engineering - VibriExpress

• Subgroup Strain Engineering - VibriInteract

• Subgroup Metabolic Engineering - Pathway Assembly

• Subgroup Metabolic Engineering - Biosensor

Protocols

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• Bradford Assay
• Cell Disruption
• Enzyme Activity Assay
• pH Tolerance Assay
• Protein Overexpression
• SDS-PAGE
• Synthesis of Acetyl-CoA and Malonyl-CoA
• Detection of 3-hydroxypropionic acid via LC-MS
• Workflow for platereader measurements
• Isolation of bacterial total DNA
• qPCR protocol
• Colony PCR
• Gibson Cloning
• Golden_Gate_Lvl0
• Golden_Gate_Lvl1
• Golden_Gate_Lvl2
• Miniprep
• PCRpurificationPCRpurification