Team:Marburg/Experiments

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Experiments

Labbooks

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https://static.igem.org/mediawiki/2018/9/97/T--Marburg--Labbook_Sequencing.pdf https://static.igem.org/mediawiki/2018/3/3f/T--Marburg--Labbook_Characterization.pdf https://static.igem.org/mediawiki/2018/4/4d/T--Marburg--Labbook_Cloning_LVL1.pdf https://static.igem.org/mediawiki/2018/9/96/T--Marburg--Labbook_Cloning_Entry_Vectors.pdf Part Collection -- Sequencing
• Subgroup Strain Engineering - General experiments
• Subgroup Strain Engineering - General experiments
• Subgroup Strain Engineering - General experiments
• Subgroup Strain Engineering - General experiments
• Subgroup Strain Engineering - General experiments

• Subgroup Strain Engineering - dns experiments

• Subgroup Strain Engineering - VibriClone

• Subgroup Strain Engineering - VibriExpress

• Subgroup Strain Engineering - VibriInteract

• Subgroup Metabolic Engineering - Pathway Assembly

• Subgroup Metabolic Engineering - Biosensor

Protocols

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• Bradford Assay
• Cell Disruption
• Enzyme Activity Assay
• pH Tolerance Assay
• Protein Overexpression
• SDS-PAGE
• Synthesis of Acetyl-CoA and Malonyl-CoA
• Detection of 3-hydroxypropionic acid via LC-MS
• Workflow for platereader measurements
• Isolation of bacterial total DNA
• qPCR protocol
• Colony PCR
• Gibson Cloning
• Golden_Gate_Lvl0
• Golden_Gate_Lvl1
• Golden_Gate_Lvl2
• Miniprep
• PCRpurification
• Q5_polymerase_PCR
• Glycerol_Stock_Preparation
• QPCR-Protocol
• Pathway_Assembly
• Genomic_DNA_Isolation
• CloningStrain