Team:Aachen/Wetlab/Protocol10

Resuspension of IDT DNA Oligonucleotide

Agarose solution 1,2 %

Procedure (1000 ml):


  1. 12 g of NEEO Agarose

  2. 20 g of 50x TAE Buffer

  3. 980 g of ddH20

  4. Mix ingedients in a 1000ml Schott-Bottles

  5. Put the bottle into the autoclave and set the program Agarose

  6. After autoclavation put the bottles into the 60 ℃ closet

  7. Write your Name and the used programm in the autoclave book

  8. Attention: Do not fill bottles with EtBr yet! Do that after opening a new bottle