Team:Aachen/Wetlab/Protocols
Protocols
During our project we used many different methods. These can be found here. They are also linked in our notebook to understand our project.
- Plasmid DNA Purification
- PCR clean-up
- Gel clean-up
- Transformation in E. coli
- Overnight culture E. coli
- Overnight culture S. cerevisiae
- Cryo culture
- Determination of DNA concentration (Nanodrop machine)
- Resuspension of IDT DNA Oligonucleotide
- Resuspension of IDT DNA Oligonucleotide Agarose solution 1,2 %
- Production of TSS-medium
- Creation of chemical competent E. coli cells
- Transformation of chemical competent E. coli cells
- E. coli Tingle Tube Transformation for Interlab Study
- Creation of Carrier DNA (400 ul)
- Cre-LOXp Treatment of yeast cells
- Making Competent Yeast Cells
- Transformation of S. cerevisiae
- Genome Isolation for S. cerevisiae
- Yeast Competence and Transformation Kit
- LB medium
- LB solid medium
- SOC medium
- YPD-Medium
- Solid YPD-Medium
- S. cerevisiae minimal medium
- S. cerevisiae minimal medium with synthetic dropout complement
- YPD-galactose-medium (solid)
- Creation of Glycerol stock
- Creation of Tris-HCl buffer (pH = 8.0)
- Creation of TE buffer (pH = 8.0)
- YPG-Medium
- Preparation of SDS page gels
- Ni-NTA affinity chromatography
- Purification of proteins1
- Coomassie
- One-step luciferase measurements in yeasts
- Yeast X-gal assay