Team:Aachen/Wetlab/Protocol15

Creation of Carrier DNA (400 ul):

Material:


  • salmon sperm: 4 mg.

  • ddH2O: 2 ml.

Method:


  1. you need to resuspend 4mg of salmon sperm in 400ul of ddH2O.

  2. deanturate DNA at 95 ℃ for 5 minutes.

  3. store carrier DNA solution at -20 ℃.


Notes:


  • salmon sperm should be autoclaved before use, however denaturation at 95 ℃ is enough.

  • salmon could be not easy to resuspend.

  • salmon sperm to weight is very difficult. It is not a problem if you cannot precisly weight the quantity you need.