Team:Aachen/Wetlab/Protocol23

SOC medium

Preparation:


  1. mix: 10 g Trypton + 1 g NaCl + 2,5 g yeast extract + 1,25 g KCl.

  2. fill up with 480 ml water.
  3. autoclave.
  4. add:

    • 5 ml 1M MgCl2 x 6H2O (1,0165 g + 5 ml ddH2O).

    • 5 ml 1M MgSO4 x 7H2O (1,2321 g + 5 ml ddH2O).
    • 10 ml 1M glucose monohydrate (1,9817 g + 10 ml ddH2O).