Team:Aachen/Wetlab/Protocol7

Cryo culture

work space:

clean bench

conservation:

-80 ℃ freezer

procedure


  1. pipette 500 uL of the glycerol stock (creation of 50% glycerol stock) in a cryo tube.

  2. (add 500 uL of the over night culture overnight culture S.cerevisiae or overnight culture e.coli.

  3. store it at -80 ℃C to conserve it.


Notes

You can transfer the ratio to every other size of the solution but 1 mL is a good and normal size for a cryo culture